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|Antigen||Tight Junction Protein 1 (Zona Occludens 1) (TJP1) Antibodies|
|Epitope||AA 463-1109 Alternatives|
|Reactivity||Dog (Canine), Guinea Pig, Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This TJP1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
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Product Details anti-TJP1 AntibodyTarget Details TJP1 Application Details Handling Images
|Specificity||Recognizes human ZO-1alpha+ and ZO- alpha- isoforms. Reactivity with dlg has not been characterized. No reactivity with PSD-95 or p55 has been observed on Western blots, but potential reactivity with these proteins has not been fully characterized. Predicted species cross-reactivity: mouse, rat, guinea pig, canine and chicken (moderate reactivity). Please note that cross-reactivity with predicted species is only predicted and is not guaranteed.|
A 69kD fusion protein corresponding to aa463-1109 of human ZO-1 cDNA. This sequence lies N-terminal to the 80 amino acid region (the alpha-motif) present in the a+-isoform but absent in the a- isoform due to alternative splicing.
Type of Immunogen: Fusion protein
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|Alternative Name||TJP1 / ZO-1 (TJP1 Antibody Abstract)|
Name/Gene ID: TJP1
Synonyms: TJP1, ZO-1, Zona occludens protein 1, Zona occludens 1, Zonula occludens protein 1, Tight junction protein 1, ZO1, Tight junction protein ZO-1, Zonula occludens 1 protein
|Pathways||Carbohydrate Homeostasis, Cell-Cell Junction Organization|
Application DetailsProduct Details anti-TJP1 Antibody Target Details TJP1 Handling Images back to top
Approved: ELISA, IHC-Fr, IHC-P (5 μg/mL), IP, WB (0.5 - 2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 μg/mL. Immunoprecipitation: 2-5 μg/reaction.
|Restrictions||For Research Use only|
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|Buffer||PBS, pH 7.4, 0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.
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