anti-Cat (Feline) Vimentin antibody for Western Blotting

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Reactivity
Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Human, Pig (Porcine)
1044
461
414
277
178
170
158
118
117
74
59
54
50
47
42
30
25
12
7
6
3
2
2
1
1
1
1
1
Host
Mouse
662
347
29
14
12
11
10
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
35
32
30
28
28
18
17
16
14
14
14
14
14
14
14
9
9
8
8
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
829
436
400
371
338
273
268
160
88
63
40
26
15
13
8
7
7
5
3
3
3
1
1
1
1
1
Supplier
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Catalog No. ABIN3026782
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6166929 FACS IF IHC (f) WB CF®594 Mouse IgG1 Log in to see VM452 0
1 ABIN6166905 FACS IF IHC (f) WB CF®405S Mouse IgG1 Log in to see VM452 0
1 ABIN6166909 FACS IF IHC (f) WB CF®640R Mouse IgG1 Log in to see VM452 0
1 ABIN6166911 FACS IF IHC (f) WB CF®543 Mouse IgG1 Log in to see VM452 0
1 ABIN6166913 FACS IF IHC (f) WB CF®647 Mouse IgG1 Log in to see VM452 0
1 ABIN6166917 FACS IF IHC (f) WB CF®660R Mouse IgG1 Log in to see VM452 0
1 ABIN6166927 FACS IF IHC (f) WB CF®488A Mouse IgG1 Log in to see VM452 0
1 ABIN6166915 FACS IF IHC (f) WB CF®555 Mouse IgG1 Log in to see VM452 0
1 ABIN6166921 FACS IF IHC (f) WB CF®770 Mouse IgG1 Log in to see VM452 0
1 ABIN6166907 FACS IF IHC (f) WB CF405M Mouse IgG1 Log in to see VM452 0
1 ABIN6166919 FACS IF IHC (f) WB CF®568 Mouse IgG1 Log in to see VM452 0
1 ABIN6166923 FACS IF IHC (f) WB CF®680 Mouse IgG1 Log in to see VM452 0
1 ABIN6166925 FACS IF IHC (f) WB CF®680R Mouse IgG1 Log in to see VM452 0
1 ABIN6166940 FACS IF IHC (f) WB Mouse IgG1 Log in to see VM452 0
1 ABIN6166932 FACS IF IHC (f) WB Alkaline Phosphatase (AP) Mouse IgG1 Log in to see VM452 0
1 ABIN6166934 FACS IF IHC (f) WB Biotin Mouse IgG1 Log in to see VM452 0
1 ABIN6166939 FACS IF IHC (f) WB PE Mouse IgG1 Log in to see VM452 0
1 ABIN6166931 FACS IF IHC (f) WB APC Mouse IgG1 Log in to see VM452 0
1 ABIN6166938 FACS IF IHC (f) WB PerCP Mouse IgG1 Log in to see VM452 0
1 ABIN6166942 FACS IF IHC (f) WB Mouse IgG1 Log in to see VM452 0

General

Antigen Vimentin (VIM) Antibodies
Reactivity Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Human, Pig (Porcine)
(1044), (461), (414), (277), (178), (170), (158), (118), (117), (74), (59), (54), (50), (47), (42), (30), (25), (12), (7), (6), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(662), (347), (29), (14), (12), (11), (10), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(35), (32), (30), (28), (28), (18), (17), (16), (14), (14), (14), (14), (14), (14), (14), (9), (9), (8), (8), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(829), (436), (400), (371), (338), (273), (268), (160), (88), (63), (40), (26), (15), (13), (8), (7), (7), (5), (3), (3), (3), (1), (1), (1), (1), (1)
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling Images
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics This antibody specifically reacts with an ~ 58 kDa protein identified as Vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin antibody alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for tumor sub-classification. Expression of vimentin, when used in conjunction with keratin antibody, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with vimentin antibody. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity with vimentin antibody is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Vimentin antibody is also useful as a tissue process control reagent.
Purification Protein G affinity chromatography
Immunogen Recombinant protein was used as the immunogen for the Vimentin antibody.
Clone VM452
Isotype IgG1
Plasmids, Primers & others

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background This antibody specifically reacts with an ~ 58  kDa protein identified as Vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin antibody alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for tumor sub-classification. Expression of vimentin, when used in conjunction with keratin antibody, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with vimentin antibody. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity with vimentin antibody is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Vimentin antibody is also useful as a tissue process control reagent.
Gene ID 7431
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling Images back to top
Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the Vimentin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Vimentin (VIM) antibody (ABIN3026782) Flow cytometry testing of Jurkat cells and Alexa Fluor 488 conjugated Vimentin antibo...
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) antibody (ABIN3026782) IHC staining of FFPE human melanoma with Vimentin antibody (VM452).
Western Blotting (WB) image for anti-Vimentin (VIM) antibody (ABIN3026782) Western blot testing of Vimentin antibody and Raji cell lysate. Predicted molecular w...