anti-Chicken Vimentin antibody for Flow Cytometry

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Reactivity
Chicken, Dog (Canine), Human, Pig (Porcine), Rat (Rattus), Sheep (Ovine)
1044
460
414
277
179
170
158
119
118
73
59
54
50
47
42
30
25
12
7
6
3
2
2
1
1
1
1
1
Host
Mouse
662
347
29
14
12
11
10
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
35
32
30
28
28
18
17
16
14
14
14
14
14
14
14
9
9
8
8
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
829
435
400
371
338
273
267
159
88
63
40
26
15
13
8
7
6
5
3
3
3
1
1
1
1
1
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Catalog No. ABIN4365312
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.667458 ABIN3026782 FACS IF IHC (p) WB Mouse IgG1 Log in to see VM452 0
1 ABIN112308 EIA FACS IHC (fro) IF IHC (p) WB FITC Mouse IgG2a Log in to see VIM 3B4 11
1 ABIN959513 FACS ICC IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see V9 0
1 ABIN959503 FACS ICC IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV202 0
1 ABIN112354 FACS IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see RV202 5
1 ABIN4365314 CyTOF FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV202 1
1 ABIN112184 FACS IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see V9 5
1 ABIN6166929 FACS IF IHC (f) WB CF®594 Mouse IgG1 Log in to see VM452 0
1 ABIN6166905 FACS IF IHC (f) WB CF®405S Mouse IgG1 Log in to see VM452 0
1 ABIN6166909 FACS IF IHC (f) WB CF®640R Mouse IgG1 Log in to see VM452 0
1 ABIN6166911 FACS IF IHC (f) WB CF®543 Mouse IgG1 Log in to see VM452 0
1 ABIN6166913 FACS IF IHC (f) WB CF®647 Mouse IgG1 Log in to see VM452 0
1 ABIN6166917 FACS IF IHC (f) WB CF®660R Mouse IgG1 Log in to see VM452 0
1 ABIN6166927 FACS IF IHC (f) WB CF®488A Mouse IgG1 Log in to see VM452 0
1 ABIN6166915 FACS IF IHC (f) WB CF®555 Mouse IgG1 Log in to see VM452 0
1 ABIN6166921 FACS IF IHC (f) WB CF®770 Mouse IgG1 Log in to see VM452 0
1 ABIN6166907 FACS IF IHC (f) WB CF405M Mouse IgG1 Log in to see VM452 0
1 ABIN6166919 FACS IF IHC (f) WB CF®568 Mouse IgG1 Log in to see VM452 0
1 ABIN6166923 FACS IF IHC (f) WB CF®680 Mouse IgG1 Log in to see VM452 0
1 ABIN6166925 FACS IF IHC (f) WB CF®680R Mouse IgG1 Log in to see VM452 0

General

Antigen Vimentin (VIM) Antibodies
Reactivity Chicken, Dog (Canine), Human, Pig (Porcine), Rat (Rattus), Sheep (Ovine)
(1044), (460), (414), (277), (179), (170), (158), (119), (118), (73), (59), (54), (50), (47), (42), (30), (25), (12), (7), (6), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(662), (347), (29), (14), (12), (11), (10), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(35), (32), (30), (28), (28), (18), (17), (16), (14), (14), (14), (14), (14), (14), (14), (9), (9), (8), (8), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(829), (435), (400), (371), (338), (273), (267), (159), (88), (63), (40), (26), (15), (13), (8), (7), (6), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN4365312) Images
Specificity V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Purification Protein A or G purified
Immunogen Clone V9 is a mouse monoclonal IgG1 antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with vimentin isolated from porcine lens.
Clone V9
Isotype IgG1
Plasmids, Primers & others

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN4365312) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Gene Symbol: VIM
Gene ID 7431
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN4365312) Images back to top
Application Notes Western Blot 1:100-1:1000, Flow Cytometry 1:25-1:200, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:25-1:200, Immunohistochemistry-Frozen 1:25-1:200, CyTOF-readyFor staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer ( pH 6.0) is required. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for Flow Cytometry, and for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN4365312) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-Vimentin antibody (ABIN4365312)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Rigoutsos, Lee, Nam, Anfossi, Pasculli, Pichler, Jing, Rodriguez-Aguayo, Telonis, Rossi, Ivan, Catela Ivkovic, Fabris, Clark, Ling, Shimizu, Redis, Shah, Zhang, Okugawa, Jung, Tsirigos, Huang, Ferdin et al.: "N-BLR, a primate-specific non-coding transcript leads to colorectal cancer invasion and migration. ..." in: Genome biology, Vol. 18, Issue 1, pp. 98, 2017

Hu, Ling, Xu, Han: "Fibroblast-like cells differentiated from adipose-derived mesenchymal stem cells for vocal fold wound healing." in: PLoS ONE, Vol. 9, Issue 3, pp. e92676, 2014 (Sample species: Dog (Canine)). Further details: Immunocytochemistry,Immunofluorescence

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN4365312) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Vimentin (VIM) antibody (ABIN4365312) Flow Cytometry: Vimentin Antibody (V9) - Analysis of PE conjugate of NBP1-97670. An ...
Flow Cytometry (FACS) image for anti-Vimentin (VIM) antibody (ABIN4365312) Flow Cytometry: Vimentin Antibody (V9) - An intracellular stain was performed on Jur...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Vimentin (VIM) antibody (ABIN4365312) Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) - Strong specific sta...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Vimentin (VIM) antibody (ABIN4365312) Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) - Strong specific sta...