anti-Chicken Vimentin antibody for Flow Cytometry

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Reactivity
Human, Rat (Rattus), Chicken, Potoroo
1005
421
380
263
170
160
149
117
110
76
63
51
47
42
28
26
24
14
9
6
3
2
2
1
1
1
1
1
1
1
1
Host
Mouse
613
355
30
14
12
11
11
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
35
31
26
25
24
16
15
12
12
12
12
12
12
12
12
9
9
7
7
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
785
413
365
335
329
270
226
134
81
63
40
26
15
13
8
7
7
4
3
3
3
1
1
1
1
1
Options
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Catalog No. ABIN335382
$ 376.36
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
23.076218 ABIN335380 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
17.076218 ABIN1042494 ICC IHC (fro) FACS IHC (p) WB HRP Mouse IgG1 Log in to see RV202 5
17.076218 ABIN1042493 ICC IHC (fro) FACS IHC (p) WB FITC Mouse IgG1 Log in to see RV202 5
12.576219 ABIN5618631 FACS IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV202 0
10 ABIN6166931 FACS IF IHC (f) WB APC Mouse IgG1 Log in to see VM452 0
10 ABIN6166939 FACS IF IHC (f) WB PE Mouse IgG1 Log in to see VM452 0
10 ABIN6166938 FACS IF IHC (f) WB PerCP Mouse IgG1 Log in to see VM452 0
10 ABIN6166942 FACS IF IHC (f) WB Mouse IgG1 Log in to see VM452 0
4 ABIN3026900 FACS IF IHC (p) WB Mouse IgG1 Log in to see SPM576 0
1 ABIN112308 EIA FACS IHC (fro) IF IHC (p) WB FITC Mouse IgG2a Log in to see VIM 3B4 11
1 ABIN112354 FACS IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see RV202 5
1 ABIN112184 FACS IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see V9 5
1 ABIN6166929 FACS IF IHC (f) WB CF®594 Mouse IgG1 Log in to see VM452 0
1 ABIN6166905 FACS IF IHC (f) WB CF®405S Mouse IgG1 Log in to see VM452 0
1 ABIN6166909 FACS IF IHC (f) WB CF®640R Mouse IgG1 Log in to see VM452 0
1 ABIN6166911 FACS IF IHC (f) WB CF®543 Mouse IgG1 Log in to see VM452 0
1 ABIN6166913 FACS IF IHC (f) WB CF®647 Mouse IgG1 Log in to see VM452 0
1 ABIN6166917 FACS IF IHC (f) WB CF®660R Mouse IgG1 Log in to see VM452 0
1 ABIN6166927 FACS IF IHC (f) WB CF®488A Mouse IgG1 Log in to see VM452 0
1 ABIN6166915 FACS IF IHC (f) WB CF®555 Mouse IgG1 Log in to see VM452 0

General

Antigen Vimentin (VIM) Antibodies
Reactivity Human, Rat (Rattus), Chicken, Potoroo
(1005), (421), (380), (263), (170), (160), (149), (117), (110), (76), (63), (51), (47), (42), (28), (26), (24), (14), (9), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(613), (355), (30), (14), (12), (11), (11), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(35), (31), (26), (25), (24), (16), (15), (12), (12), (12), (12), (12), (12), (12), (12), (9), (9), (7), (7), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(785), (413), (365), (335), (329), (270), (226), (134), (81), (63), (40), (26), (15), (13), (8), (7), (7), (4), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN335382) Images
Specificity Human, rat, chicken and potoroo.
Purification Purified
Immunogen V9 is a mouse monoclonal IgG1 antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with vimentin isolated from porcine lens.
Clone V9
Isotype IgG1
Plasmids, Primers & others

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN335382) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN335382) Images back to top
Application Notes V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. V9 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. For staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer (pH 6.0) is required. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN335382) Images back to top
Storage 4 °C

References for anti-Vimentin antibody (ABIN335382)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

De Visscher, Plusquin, Mesure, Flameng: "Selection of an immunohistochemical panel for cardiovascular research in sheep." in: Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry, Vol. 18, Issue 4, pp. 382-91, 2010

Corver, Koopman, van der Aa, Regensburg, Fleuren, Cornelisse: "Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer." in: Cytometry, Vol. 39, Issue 2, pp. 96-107, 2000

Van Muijen, Ruiter, Warnaar: "Coexpression of intermediate filament polypeptides in human fetal and adult tissues." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 57, Issue 4, pp. 359-69, 1987

Van Muijen, Warnaar, Ponec: "Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis." in: Experimental cell research, Vol. 171, Issue 2, pp. 331-45, 1987

Tölle, Weber, Osborn: "Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple keratins allow insight into the composition of particular 10 nm filaments." in: European journal of cell biology, Vol. 38, Issue 2, pp. 234-44, 1985

Osborn, Debus, Weber: "Monoclonal antibodies specific for vimentin." in: European journal of cell biology, Vol. 34, Issue 1, pp. 137-43, 1984

Background publications

Brussee, Smit, Koopman, Wijga, Kerkhof, Corver, Vos, Gerritsen, Grobbee, Brunekreef, Merkus, de Jongste: "Interrupter resistance and wheezing phenotypes at 4 years of age." in: American journal of respiratory and critical care medicine, Vol. 169, Issue 2, pp. 209-13, 2004

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN335382) back to top
Supplier Images
 image for anti-Vimentin (VIM) antibody (ABIN335382) anti-Vimentin (VIM) antibody
 image for anti-Vimentin (VIM) antibody (ABIN335382) anti-Vimentin (VIM) antibody (Image 2)
 image for anti-Vimentin (VIM) antibody (ABIN335382) anti-Vimentin (VIM) antibody (Image 3)