anti-Dog (Canine) Vimentin antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Reactivity
Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Human, Pig (Porcine)
1004
422
380
262
169
160
148
116
109
76
62
51
47
42
28
26
24
14
9
6
3
2
2
2
1
1
1
1
1
1
1
Host
Mouse
613
355
28
14
12
11
11
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is conjugated to APC
35
31
26
25
23
16
15
12
12
12
12
12
12
12
12
9
9
7
7
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
784
412
363
336
329
270
227
135
81
62
40
26
15
13
8
7
7
4
3
3
3
1
1
1
1
1
Supplier
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Catalog No. ABIN6166931
$ 392.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10 ABIN6166939 FACS IF IHC (f) WB PE Mouse IgG1 Log in to see VM452 0
10 ABIN6166938 FACS IF IHC (f) WB PerCP Mouse IgG1 Log in to see VM452 0
10 ABIN6166942 FACS IF IHC (f) WB Mouse IgG1 Log in to see VM452 0
1 ABIN6166929 FACS IF IHC (f) WB CF®594 Mouse IgG1 Log in to see VM452 0
1 ABIN6166905 FACS IF IHC (f) WB CF®405S Mouse IgG1 Log in to see VM452 0
1 ABIN6166909 FACS IF IHC (f) WB CF®640R Mouse IgG1 Log in to see VM452 0
1 ABIN6166911 FACS IF IHC (f) WB CF®543 Mouse IgG1 Log in to see VM452 0
1 ABIN6166913 FACS IF IHC (f) WB CF®647 Mouse IgG1 Log in to see VM452 0
1 ABIN6166917 FACS IF IHC (f) WB CF®660R Mouse IgG1 Log in to see VM452 0
1 ABIN6166927 FACS IF IHC (f) WB CF®488A Mouse IgG1 Log in to see VM452 0
1 ABIN6166915 FACS IF IHC (f) WB CF®555 Mouse IgG1 Log in to see VM452 0
1 ABIN6166921 FACS IF IHC (f) WB CF®770 Mouse IgG1 Log in to see VM452 0
1 ABIN6166907 FACS IF IHC (f) WB CF405M Mouse IgG1 Log in to see VM452 0
1 ABIN6166919 FACS IF IHC (f) WB CF®568 Mouse IgG1 Log in to see VM452 0
1 ABIN6166923 FACS IF IHC (f) WB CF®680 Mouse IgG1 Log in to see VM452 0
1 ABIN6166925 FACS IF IHC (f) WB CF®680R Mouse IgG1 Log in to see VM452 0
1 ABIN6166940 FACS IF IHC (f) WB Mouse IgG1 Log in to see VM452 0
1 ABIN6166932 FACS IF IHC (f) WB Alkaline Phosphatase (AP) Mouse IgG1 Log in to see VM452 0
1 ABIN6166934 FACS IF IHC (f) WB Biotin Mouse IgG1 Log in to see VM452 0
1 ABIN6166936 FACS IF IHC (f) WB HRP Mouse IgG1 Log in to see VM452 0

General

Antigen Vimentin (VIM) Antibodies
Reactivity Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Human, Pig (Porcine)
(1004), (422), (380), (262), (169), (160), (148), (116), (109), (76), (62), (51), (47), (42), (28), (26), (24), (14), (9), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(613), (355), (28), (14), (12), (11), (11), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is conjugated to APC
(35), (31), (26), (25), (23), (16), (15), (12), (12), (12), (12), (12), (12), (12), (12), (9), (9), (7), (7), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
(784), (412), (363), (336), (329), (270), (227), (135), (81), (62), (40), (26), (15), (13), (8), (7), (7), (4), (3), (3), (3), (1), (1), (1), (1), (1)
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling Images
Purpose Mouse Monoclonal anti-Vimentin (VM452), APC Conjugate
Characteristics This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Recombinant full-length human vimentin protein
Clone VM452
Isotype IgG1
Plasmids, Primers & others

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background VIM
Molecular Weight 57-60 kDa
Gene ID 7431, 455493
UniProt P08670
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling Images back to top
Application Notes Does not react with mouse or rat, others not known
  • Immunohistology formalin-fixed 0.25-0.5 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Immunofluorescence 1-2 μg/mL
  • Western blotting 0.25-0.5 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling back to top
Supplier Images
 image for anti-Vimentin (VIM) antibody (APC) (ABIN6166931) anti-Vimentin (VIM) antibody (APC)
 image for anti-Vimentin (VIM) antibody (APC) (ABIN6166931) anti-Vimentin (VIM) antibody (APC) (Image 2)
 image for anti-Vimentin (VIM) antibody (APC) (ABIN6166931) anti-Vimentin (VIM) antibody (APC) (Image 3)