anti-Human Vimentin antibody for Enzyme Immunoassay

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Epitope
Middle Region
40
25
24
22
21
18
15
15
14
8
7
7
6
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
928
406
356
221
138
132
121
83
81
52
51
43
41
37
30
12
8
7
5
5
4
3
3
2
2
1
1
1
1
1
Host
Rabbit
570
320
30
17
12
9
9
6
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
29
27
26
23
18
16
13
13
13
13
13
13
13
13
12
11
7
7
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
739
344
328
305
255
252
250
144
87
40
15
14
13
10
8
6
5
3
3
3
1
1
1
1
1
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Catalog No. ABIN357888
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN120734 EIA IF WB Mouse IgG1 Log in to see VI-RE-1 1
1 ABIN5552109 EIA WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
1 ABIN112306 EIA IHC (fro) IF IHC (p) WB Mouse IgG2a Log in to see VIM 3B4 10
1 ABIN1109482 EIA IHC (p) WB Mouse IgG1 Log in to see 9E7E7 3
1 ABIN5552108 EIA IHC (p) WB Rabbit Ig Fraction Log in to see Polyclonal
1 ABIN112308 EIA FACS IHC (fro) IF IHC (p) WB FITC Mouse IgG2a Log in to see VIM 3B4 11

General

Antigen Vimentin (VIM) Antibodies
Epitope Middle Region
(40), (25), (24), (22), (21), (18), (15), (15), (14), (8), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(928), (406), (356), (221), (138), (132), (121), (83), (81), (52), (51), (43), (41), (37), (30), (12), (8), (7), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(570), (320), (30), (17), (12), (9), (9), (6)
Clonality
Polyclonal
Conjugate This Vimentin antibody is un-conjugated
(29), (27), (26), (23), (18), (16), (13), (13), (13), (13), (13), (13), (13), (13), (12), (11), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(739), (344), (328), (305), (255), (252), (250), (144), (87), (40), (15), (14), (13), (10), (8), (6), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN357888) Images
Specificity This antibody detects Vimentin (VIM) (Center).
Purification Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human Vimentin.
Isotype Ig

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN357888) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.Synonyms: VIM
Molecular Weight 53652 Da
Gene ID 7431, 9606
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN357888) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/50-1/100Immunohistochemistry: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN357888) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-Vimentin antibody (ABIN357888)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Whipple, Balzer, Cho, Matrone, Yoon, Martin: "Vimentin filaments support extension of tubulin-based microtentacles in detached breast tumor cells." in: Cancer research, Vol. 68, Issue 14, pp. 5678-88, 2008

Garcia-Verdugo, Synguelakis, Degrouard, Franco, Valot, Zivy, Chaby, Tanfin: "Interaction of surfactant protein A with the intermediate filaments desmin and vimentin." in: Biochemistry, Vol. 47, Issue 18, pp. 5127-38, 2008

Merdes, Brunkener, Horstmann, Georgatos: "Filensin: a new vimentin-binding, polymerization-competent, and membrane-associated protein of the lens fiber cell." in: The Journal of cell biology, Vol. 115, Issue 2, pp. 397-410, 1991

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN357888) back to top
Supplier Images
 image for anti-Vimentin (VIM) (Middle Region) antibody (ABIN357888) anti-Vimentin (VIM) (Middle Region) antibody
 image for anti-Vimentin (VIM) (Middle Region) antibody (ABIN357888) anti-Vimentin (VIM) (Middle Region) antibody (Image 2)