anti-Human Vimentin antibody for Enzyme Immunoassay

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Epitope
Middle Region
28
26
23
22
21
18
16
16
15
13
8
6
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1021
428
386
260
168
159
147
115
108
76
63
51
47
42
28
27
24
14
9
6
3
2
2
2
1
1
1
1
1
1
1
Host
Rabbit
619
366
31
14
12
11
11
5
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
35
31
26
25
24
16
15
12
12
12
12
12
12
12
12
9
9
7
7
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
803
419
369
334
331
275
226
135
86
63
40
26
15
13
8
6
6
3
3
3
3
2
1
1
1
1
1
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Catalog No. ABIN357888
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN1109482 EIA IHC (p) WB Mouse IgG1 Log in to see 9E7E7 3
5.5 ABIN120734 EIA IF WB Mouse IgG1 Log in to see VI-RE-1 1
1 ABIN112306 EIA IHC (fro) IF IHC (p) WB Mouse IgG2a Log in to see VIM 3B4 10
1 ABIN112308 EIA FACS IHC (fro) IF IHC (p) WB FITC Mouse IgG2a Log in to see VIM 3B4 11
1 ABIN5552109 EIA WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 0
1 ABIN5552108 EIA IHC (p) WB Rabbit Ig Fraction Log in to see Polyclonal 0

General

Antigen Vimentin (VIM) Antibodies
Epitope Middle Region
(28), (26), (23), (22), (21), (18), (16), (16), (15), (13), (8), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1021), (428), (386), (260), (168), (159), (147), (115), (108), (76), (63), (51), (47), (42), (28), (27), (24), (14), (9), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(619), (366), (31), (14), (12), (11), (11), (5)
Clonality
Polyclonal
Conjugate This Vimentin antibody is un-conjugated
(35), (31), (26), (25), (24), (16), (15), (12), (12), (12), (12), (12), (12), (12), (12), (9), (9), (7), (7), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(803), (419), (369), (334), (331), (275), (226), (135), (86), (63), (40), (26), (15), (13), (8), (6), (6), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN357888) Images
Specificity This antibody detects Vimentin (VIM) (Center).
Purification Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human Vimentin.
Isotype Ig Fraction
Plasmids, Primers & others

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN357888) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.Synonyms: VIM
Molecular Weight 53652 Da
Gene ID 7431, 9606
UniProt P08670
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN357888) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/50-1/100Immunohistochemistry: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN357888) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-Vimentin antibody (ABIN357888)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Whipple, Balzer, Cho, Matrone, Yoon, Martin: "Vimentin filaments support extension of tubulin-based microtentacles in detached breast tumor cells." in: Cancer research, Vol. 68, Issue 14, pp. 5678-88, 2008

Garcia-Verdugo, Synguelakis, Degrouard, Franco, Valot, Zivy, Chaby, Tanfin: "Interaction of surfactant protein A with the intermediate filaments desmin and vimentin." in: Biochemistry, Vol. 47, Issue 18, pp. 5127-38, 2008

Merdes, Brunkener, Horstmann, Georgatos: "Filensin: a new vimentin-binding, polymerization-competent, and membrane-associated protein of the lens fiber cell." in: The Journal of cell biology, Vol. 115, Issue 2, pp. 397-410, 1991

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN357888) back to top
Supplier Images
 image for anti-Vimentin (VIM) (Middle Region) antibody (ABIN357888) anti-Vimentin (VIM) (Middle Region) antibody
 image for anti-Vimentin (VIM) (Middle Region) antibody (ABIN357888) anti-Vimentin (VIM) (Middle Region) antibody (Image 2)