anti-Mammalian Vimentin antibody for Simple Western

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Reactivity
Human, Mammalian, Mouse (Murine), Rat (Rattus)
1044
460
413
278
179
171
159
119
118
74
59
54
50
47
41
30
25
12
7
6
3
2
2
1
1
1
1
1
Host
Mouse
662
347
29
14
12
11
10
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
35
32
30
28
28
18
17
16
14
14
14
14
14
14
14
9
9
8
8
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
829
435
400
372
339
273
267
160
88
63
40
26
15
13
8
7
7
4
3
3
3
1
1
1
1
1
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Available images

Catalog No. ABIN4365308
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4365307 ICC IF IHC SimWes WB Mouse IgG2a kappa Log in to see 2D1 0

General

Antigen Vimentin (VIM) Antibodies
Reactivity Human, Mammalian, Mouse (Murine), Rat (Rattus)
(1044), (460), (413), (278), (179), (171), (159), (119), (118), (74), (59), (54), (50), (47), (41), (30), (25), (12), (7), (6), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(662), (347), (29), (14), (12), (11), (10), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(35), (32), (30), (28), (28), (18), (17), (16), (14), (14), (14), (14), (14), (14), (14), (9), (9), (8), (8), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
(829), (435), (400), (372), (339), (273), (267), (160), (88), (63), (40), (26), (15), (13), (8), (7), (7), (4), (3), (3), (3), (1), (1), (1), (1), (1)
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling Images
Purification Protein G purified
Immunogen Recombinant human Vimentin expressed in and purified from E. coli. [UniProt# P08670]
Clone 2D1
Isotype IgG2a kappa
Plasmids, Primers & others

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Gene Symbol: VIM
Molecular Weight Theoretical MW: 50 kDa
Gene ID 7431
UniProt P08670
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling Images back to top
Application Notes Western Blot 1:5000-1:10000, Simple Western 1:100, Immunohistochemistry 1:1000, Immunocytochemistry/Immunofluorescence 1:500-1:1000This Vimentin (2D1) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 50 kDa. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 10 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) antibody (ABIN4365308) Immunohistochemistry: Vimentin Antibody (2D1) - Mouse cortex microslice section (fro...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Vimentin (VIM) antibody (ABIN4365308) Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2D1) - Analysis of cortic...
Western Blotting (WB) image for anti-Vimentin (VIM) antibody (ABIN4365308) Western Blot: Vimentin Antibody (2D1) - Analysis of different cell lysates using mou...
 image for anti-Vimentin (VIM) antibody (ABIN4365308) Knockdown Validated: Vimentin Antibody (2D1) - Western blot shows lysates of K562 hu...