anti-Mouse (Murine) Vimentin antibody for Immunofluorescence

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Epitope
Center
28
25
23
22
19
18
16
16
15
13
8
7
6
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1004
421
379
263
170
161
149
117
110
76
62
51
47
42
28
26
24
14
9
6
3
2
2
2
1
1
1
1
1
1
1
Host
Rabbit
614
354
28
14
12
11
11
5
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
35
31
26
25
24
16
15
12
12
12
12
12
12
12
12
9
9
7
7
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
784
412
363
335
330
270
226
134
80
63
40
26
15
13
8
7
7
4
3
3
3
1
1
1
1
1
Options
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Catalog No. ABIN2854814
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
32.5 ABIN6269441 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 14
20.313196 ABIN389211 IF IHC (p) WB Rabbit Ig Fraction AA 63-90, Ser82 Log in to see Polyclonal 3
18.57224 ABIN2613493 ELISA FACS IF WB Goat C-Term Log in to see Polyclonal 0
18.57224 ABIN3187471 ELISA IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
17.313196 ABIN1875329 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
17.313196 ABIN5963996 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
15.572241 ABIN3187473 ELISA IF IHC WB Rabbit IgG Tyr319 Log in to see Polyclonal 0
15.572241 ABIN5663630 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
14.5 ABIN6265978 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 1
14.313196 ABIN2705329 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal 0
14.313196 ABIN2852672 IC IF IHC WB Mouse Log in to see 0
14.313196 ABIN1875330 IF WB Rabbit IgG Log in to see Polyclonal 0
14.313196 ABIN3029590 IF IHC ELISA WB Rabbit Ig Fraction AA 63-90 Log in to see Polyclonal 0
11.5 ABIN6265980 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 3
11.313196 ABIN1109487 IF WB Mouse IgG2b Log in to see MO82 14
11.313196 ABIN1109485 IF WB Mouse IgG2b pSer55 Log in to see 4A4 11
11.313196 ABIN1109486 IF WB Rat IgG2a Log in to see TM71 9
11.313196 ABIN1449160 IF WB Rat IgG2a pSer50 Log in to see TM50 7
11.313196 ABIN2707782 IC IF WB Rabbit N-Term Log in to see Polyclonal 0
11.313196 ABIN955516 IF IHC (p) WB Chicken Ig Fraction Log in to see Polyclonal 0

General

Antigen Vimentin (VIM) Antibodies
Epitope Center
(28), (25), (23), (22), (19), (18), (16), (16), (15), (13), (8), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1004), (421), (379), (263), (170), (161), (149), (117), (110), (76), (62), (51), (47), (42), (28), (26), (24), (14), (9), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(614), (354), (28), (14), (12), (11), (11), (5)
Clonality
Polyclonal
Conjugate This Vimentin antibody is un-conjugated
(35), (31), (26), (25), (24), (16), (15), (12), (12), (12), (12), (12), (12), (12), (12), (9), (9), (7), (7), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(784), (412), (363), (335), (330), (270), (226), (134), (80), (63), (40), (26), (15), (13), (8), (7), (7), (4), (3), (3), (3), (1), (1), (1), (1), (1)
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling Images
Cross-Reactivity Chimpanzee
Cross-Reactivity (Details) Chimpanzee (100 %)
Characteristics Rabbit Polyclonal antibody to Vimentin (vimentin)
Vimentin antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Molecular Weight 54 kDa
Gene ID 7431
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Frozen sections) 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:20000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Frozen sections)1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:20000*
Comment

Positive Control: 293T , HeLa , NIH-3T3 , PC-12 , Rat2 , *A549

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) IHC-Fr Image Immunofluorescence photomicrographs of frozen sections of mouse brain. G...
Immunoprecipitation (IP) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) IP Image Immunoprecipitation of Vimentin protein from HeLa whole cell extracts using ...
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) IHC-P Image Vimentin antibody detects Vimentin protein at cytosol on mouse thymus gla...
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) IHC-P Image Vimentin antibody detects Vimentin protein at cytoplasm in rat testis by ...
Western Blotting (WB) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) WB Image Vimentin antibody detects Vimentin protein by western blot analysis. A. 30 μ...
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using V...
Western Blotting (WB) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) WB Image Vimentin antibody detects Vimentin protein by western blot analysis. Various...
Western Blotting (WB) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) WB Image Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the ...
Immunofluorescence (IF) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed H...
Western Blotting (WB) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) WB Image Vimentin antibody detects Vimentin protein by western blot analysis. A. 20 μ...