anti-Rat (Rattus) Vimentin antibody for Immunoprecipitation

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Epitope
Center
28
25
23
22
19
18
16
16
15
13
8
7
6
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1005
421
379
263
170
161
149
117
110
76
63
51
47
42
28
26
24
14
9
6
3
2
2
2
1
1
1
1
1
1
1
Host
Rabbit
614
354
30
14
12
11
11
5
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
35
31
26
25
24
16
15
12
12
12
12
12
12
12
12
9
9
7
7
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
785
414
364
335
330
270
226
134
80
63
40
26
15
13
8
7
7
4
3
3
3
1
1
1
1
1
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Catalog No. ABIN2854814
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
35.462074 ABIN94492 ICC IHC (p) IP WB Mouse IgM Log in to see VI-10 1
12.755764 ABIN1690704 IP Sepharose Mouse IgG1 Log in to see VMT-24 0
12.755764 ABIN1690357 IP Magnetic Particles Mouse IgG1 Log in to see VMT-24 0
7 ABIN957759 IHC IHC (p) IP WB Mouse IgG3, kappa Log in to see 23H2 0
7 ABIN957763 ELISA IHC IHC (p) IP WB Mouse IgG1, kappa Log in to see 33C7 0
7 ABIN239818 ICC IHC IHC (p) IP WB Mouse IgM Log in to see VI-10 0
4 ABIN127264 IHC (p) IP WB Rabbit Log in to see Polyclonal 0
4 ABIN1502034 IP Magnetic Particles Mouse IgG1 Log in to see VMT-24 0
4 ABIN1724173 ICC IHC (p) IP WB Mouse IgM Log in to see VI-10 0
4 ABIN1724499 IHC (p) IP WB Mouse IgG3 kappa Log in to see 23H2 0
4 ABIN1724500 IHC (p) IP ELISA WB Mouse IgG1 kappa Log in to see 33C7 0
4 ABIN2469991 ICC IP IHC WB Mouse IgM Log in to see VI-10 0
1 ABIN1502035 IP Sepharose Mouse IgG1 Log in to see VMT-24 0
1 ABIN1820967 IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN120783 IF IHC (p) IP WB Mouse IgM Log in to see VI-10 0
1 ABIN3200820 ICC IHC (p) IP WB Mouse IgM Log in to see VI-10 0
1 ABIN2471531 IP IHC ELISA WB Mouse IgG1 kappa Log in to see 33C7 0
1 ABIN2471532 IP IHC WB Mouse IgG3 kappa Log in to see 23H2 0
1 ABIN5012426 IP WB Mouse IgG1 kappa Log in to see 33C7 0
1 ABIN5012422 IP WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Vimentin (VIM) Antibodies
Epitope Center
(28), (25), (23), (22), (19), (18), (16), (16), (15), (13), (8), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1005), (421), (379), (263), (170), (161), (149), (117), (110), (76), (63), (51), (47), (42), (28), (26), (24), (14), (9), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(614), (354), (30), (14), (12), (11), (11), (5)
Clonality
Polyclonal
Conjugate This Vimentin antibody is un-conjugated
(35), (31), (26), (25), (24), (16), (15), (12), (12), (12), (12), (12), (12), (12), (12), (9), (9), (7), (7), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(785), (414), (364), (335), (330), (270), (226), (134), (80), (63), (40), (26), (15), (13), (8), (7), (7), (4), (3), (3), (3), (1), (1), (1), (1), (1)
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling Images
Cross-Reactivity Chimpanzee
Cross-Reactivity (Details) Chimpanzee (100 %)
Characteristics Rabbit Polyclonal antibody to Vimentin (vimentin)
Vimentin antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Molecular Weight 54 kDa
Gene ID 7431
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Frozen sections) 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:20000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Frozen sections)1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:20000*
Comment

Positive Control: 293T , HeLa , NIH-3T3 , PC-12 , Rat2 , *A549

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) IHC-Fr Image Immunofluorescence photomicrographs of frozen sections of mouse brain. G...
Immunoprecipitation (IP) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) IP Image Immunoprecipitation of Vimentin protein from HeLa whole cell extracts using ...
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) IHC-P Image Vimentin antibody detects Vimentin protein at cytosol on mouse thymus gla...
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) IHC-P Image Vimentin antibody detects Vimentin protein at cytoplasm in rat testis by ...
Western Blotting (WB) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) WB Image Vimentin antibody detects Vimentin protein by western blot analysis. A. 30 μ...
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using V...
Western Blotting (WB) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) WB Image Vimentin antibody detects Vimentin protein by western blot analysis. Various...
Western Blotting (WB) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) WB Image Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the ...
Immunofluorescence (IF) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed H...
Western Blotting (WB) image for anti-Vimentin (VIM) (Center) antibody (ABIN2854814) WB Image Vimentin antibody detects Vimentin protein by western blot analysis. A. 20 μ...