anti-Sheep (Ovine) Vimentin antibody for Flow Cytometry

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Reactivity
Chicken, Dog (Canine), Human, Pig (Porcine), Rat (Rattus), Sheep (Ovine)
1090
479
433
309
213
185
173
151
133
90
76
54
50
47
42
30
25
12
7
6
3
2
2
1
1
1
1
1
Host
Mouse
708
347
29
14
12
11
10
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
38
32
31
30
28
18
17
16
14
14
14
14
14
14
14
10
9
9
8
8
6
6
6
6
6
6
6
6
6
6
6
6
6
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
858
448
431
388
371
273
266
159
114
87
40
27
15
13
8
7
6
5
3
3
3
1
1
1
1
1
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Catalog No. ABIN4365312
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN959513 FACS ICC IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see V9 0
1 ABIN112184 FACS IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see V9 5
1 ABIN6166841 FACS IF IHC (f) CF®660R Mouse IgM Log in to see LN-6 0
1 ABIN6166842 FACS IF IHC (f) CF®660R Mouse IgM Log in to see LN-6 0
1 ABIN6166843 FACS IF IHC (f) CF®568 Mouse IgM Log in to see LN-6 0
1 ABIN6166844 FACS IF IHC (f) CF®568 Mouse IgM Log in to see LN-6 0
1 ABIN6166845 FACS IF IHC (f) CF®770 Mouse IgM Log in to see LN-6 0
1 ABIN6166846 FACS IF IHC (f) CF®770 Mouse IgM Log in to see LN-6 0
1 ABIN6166847 FACS IF IHC (f) CF®680 Mouse IgM Log in to see LN-6 0
1 ABIN6166848 FACS IF IHC (f) CF®680 Mouse IgM Log in to see LN-6 0
1 ABIN6166849 FACS IF IHC (f) CF®680R Mouse IgM Log in to see LN-6 0
1 ABIN6166850 FACS IF IHC (f) CF®680R Mouse IgM Log in to see LN-6 0
1 ABIN6166851 FACS IF IHC (f) CF®488A Mouse IgM Log in to see LN-6 0
1 ABIN6166852 FACS IF IHC (f) CF®488A Mouse IgM Log in to see LN-6 0
1 ABIN6166829 FACS IF IHC (f) CF®405S Mouse IgM Log in to see LN-6 0
1 ABIN6166830 FACS IF IHC (f) CF®405S Mouse IgM Log in to see LN-6 0
1 ABIN6166831 FACS IF IHC (f) CF405M Mouse IgM Log in to see LN-6 0
1 ABIN6166832 FACS IF IHC (f) CF405M Mouse IgM Log in to see LN-6 0
1 ABIN6166833 FACS IF IHC (f) CF®640R Mouse IgM Log in to see LN-6 0
1 ABIN6166834 FACS IF IHC (f) CF®640R Mouse IgM Log in to see LN-6 0

General

Antigen Vimentin (VIM) Antibodies
Reactivity Chicken, Dog (Canine), Human, Pig (Porcine), Rat (Rattus), Sheep (Ovine)
(1090), (479), (433), (309), (213), (185), (173), (151), (133), (90), (76), (54), (50), (47), (42), (30), (25), (12), (7), (6), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(708), (347), (29), (14), (12), (11), (10), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(38), (32), (31), (30), (28), (18), (17), (16), (14), (14), (14), (14), (14), (14), (14), (10), (9), (9), (8), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(858), (448), (431), (388), (371), (273), (266), (159), (114), (87), (40), (27), (15), (13), (8), (7), (6), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN4365312) Images
Specificity V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Purification Protein A or G purified
Immunogen Clone V9 is a mouse monoclonal IgG1 antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with vimentin isolated from porcine lens.
Clone V9
Isotype IgG1
Plasmids, Primers & others

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN4365312) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Gene Symbol: VIM
Gene ID 7431
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN4365312) Images back to top
Application Notes Western Blot 1:100-1:1000, Flow Cytometry 1:25-1:200, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:25-1:200, Immunohistochemistry-Frozen 1:25-1:200, CyTOF-readyFor staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer ( pH 6.0) is required. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for Flow Cytometry, and for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN4365312) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-Vimentin antibody (ABIN4365312)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Rigoutsos, Lee, Nam, Anfossi, Pasculli, Pichler, Jing, Rodriguez-Aguayo, Telonis, Rossi, Ivan, Catela Ivkovic, Fabris, Clark, Ling, Shimizu, Redis, Shah, Zhang, Okugawa, Jung, Tsirigos, Huang, Ferdin et al.: "N-BLR, a primate-specific non-coding transcript leads to colorectal cancer invasion and migration. ..." in: Genome biology, Vol. 18, Issue 1, pp. 98, 2017

Hu, Ling, Xu, Han: "Fibroblast-like cells differentiated from adipose-derived mesenchymal stem cells for vocal fold wound healing." in: PLoS ONE, Vol. 9, Issue 3, pp. e92676, 2014 (Sample species: Dog (Canine)). Further details: Immunocytochemistry,Immunofluorescence

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN4365312) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Vimentin (VIM) antibody (ABIN4365312) Flow Cytometry: Vimentin Antibody (V9) - Analysis of PE conjugate of NBP1-97670. An ...
Flow Cytometry (FACS) image for anti-Vimentin (VIM) antibody (ABIN4365312) Flow Cytometry: Vimentin Antibody (V9) - An intracellular stain was performed on Jur...