anti-Sheep (Ovine) Vimentin antibody for Immunocytochemistry

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Reactivity
Chicken, Dog (Canine), Human, Pig (Porcine), Rat (Rattus), Sheep (Ovine)
1039
462
416
275
179
168
156
117
116
73
59
54
50
47
42
30
25
12
7
6
3
2
2
1
1
1
1
1
Host
Mouse
657
347
29
14
12
11
10
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
35
32
30
28
28
18
17
16
14
14
14
14
14
14
14
9
9
8
8
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
824
430
397
371
336
273
266
159
87
63
40
26
15
13
8
7
6
5
3
3
3
1
1
1
1
1
Supplier
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Catalog No. ABIN4365312
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN959513 FACS ICC IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see V9 0
1 ABIN2964611 ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 full length Log in to see V9 0
1 ABIN4365367 FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG1 Log in to see V9 0
1 ABIN4365334 FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgG1 Log in to see V9 0
1 ABIN4365357 FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgG1 Log in to see V9 0
1 ABIN4365329 FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgG1 Log in to see V9 0
1 ABIN4365375 FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgG1 Log in to see V9 0
1 ABIN4365387 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 Log in to see V9 0
1 ABIN4365393 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 Log in to see V9 0
1 ABIN4365405 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG1 Log in to see V9 0
1 ABIN4365399 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG1 Log in to see V9 0
1 ABIN1818034 ICC WB Rat IgG2a pSer38 Log in to see TM38 0
1 ABIN1818039 ICC WB Mouse IgG1 pSer33 Log in to see YT33 0
1 ABIN5947615 FACS ICC IF IHC IHC (fro) IHC (p) Janelia Fluor® 549 Mouse IgG1 Log in to see V9 0
1 ABIN5947616 FACS ICC IF IHC IHC (fro) IHC (p) Janelia Fluor® 646 Mouse IgG1 Log in to see V9 0

General

Antigen Vimentin (VIM) Antibodies
Reactivity Chicken, Dog (Canine), Human, Pig (Porcine), Rat (Rattus), Sheep (Ovine)
(1039), (462), (416), (275), (179), (168), (156), (117), (116), (73), (59), (54), (50), (47), (42), (30), (25), (12), (7), (6), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(657), (347), (29), (14), (12), (11), (10), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(35), (32), (30), (28), (28), (18), (17), (16), (14), (14), (14), (14), (14), (14), (14), (9), (9), (8), (8), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(824), (430), (397), (371), (336), (273), (266), (159), (87), (63), (40), (26), (15), (13), (8), (7), (6), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN4365312) Images
Specificity V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Purification Protein A or G purified
Immunogen Clone V9 is a mouse monoclonal IgG1 antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with vimentin isolated from porcine lens.
Clone V9
Isotype IgG1
Plasmids, Primers & others

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN4365312) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Gene Symbol: VIM
Gene ID 7431
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN4365312) Images back to top
Application Notes Western Blot 1:100-1:1000, Flow Cytometry 1:25-1:200, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:25-1:200, Immunohistochemistry-Frozen 1:25-1:200, CyTOF-readyFor staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer ( pH 6.0) is required. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for Flow Cytometry, and for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN4365312) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-Vimentin antibody (ABIN4365312)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Rigoutsos, Lee, Nam, Anfossi, Pasculli, Pichler, Jing, Rodriguez-Aguayo, Telonis, Rossi, Ivan, Catela Ivkovic, Fabris, Clark, Ling, Shimizu, Redis, Shah, Zhang, Okugawa, Jung, Tsirigos, Huang, Ferdin et al.: "N-BLR, a primate-specific non-coding transcript leads to colorectal cancer invasion and migration. ..." in: Genome biology, Vol. 18, Issue 1, pp. 98, 2017

Hu, Ling, Xu, Han: "Fibroblast-like cells differentiated from adipose-derived mesenchymal stem cells for vocal fold wound healing." in: PLoS ONE, Vol. 9, Issue 3, pp. e92676, 2014 (Sample species: Dog (Canine)). Further details: Immunocytochemistry,Immunofluorescence

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN4365312) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Vimentin (VIM) antibody (ABIN4365312) Flow Cytometry: Vimentin Antibody (V9) - Analysis of PE conjugate of NBP1-97670. An ...
Flow Cytometry (FACS) image for anti-Vimentin (VIM) antibody (ABIN4365312) Flow Cytometry: Vimentin Antibody (V9) - An intracellular stain was performed on Jur...