anti-Human CDKN1B antibody for Enzyme Immunoassay

Recommended CDKN1B Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
  • CDKN4
  • KIP1
  • MEN1B
  • MEN4
  • P27KIP1
  • cdkn1b
  • sb:cb611
  • wu:fb63g10
  • wu:fb64g10
  • wu:fe18e03
  • CDKN1B
  • Kip1
  • p27
  • Cdki1b
  • AA408329
  • AI843786
  • p27Kip1
  • cdkn1bl
  • cyclin dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Bb
  • cyclin-dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Ba
  • CDKN1B
  • cdkn1bb
  • Cdkn1b
  • cdkn1ba
Epitope
pThr198
82
55
47
42
35
28
24
20
19
19
19
19
17
16
14
13
13
10
9
8
7
7
6
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
789
439
375
182
42
24
18
8
8
7
3
3
2
1
1
1
Host
Rabbit
480
307
37
2
2
1
Clonality
Polyclonal
Conjugate
This CDKN1B antibody is un-conjugated
41
36
35
34
34
29
9
8
8
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA)
629
338
243
233
201
198
101
98
84
55
39
28
13
7
5
3
1
1
1
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Catalog No. ABIN358459
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN358766 EIA IF WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 5
1 ABIN358183 EIA IHC (p) WB Rabbit Ig Fraction pSer10 Log in to see Polyclonal 5
1 ABIN358185 EIA IHC (p) WB Rabbit Ig Fraction pThr157 Log in to see Polyclonal 5
1 ABIN358186 EIA IHC (p) WB Rabbit Ig Fraction pThr187 Log in to see Polyclonal 5
1 ABIN358184 EIA IHC (p) WB Rabbit Ig Fraction pSer178 Log in to see Polyclonal 5
1 ABIN5552643 EIA WB Goat C-Term Log in to see Polyclonal 0
1 ABIN784207 EIA IHC (p) WB Rabbit IgG pThr187 Log in to see Polyclonal 0
1 ABIN781973 EIA IF IHC (p) WB Mouse IgG1 AA 1-199 Log in to see 4B4-E6 0
1 ABIN615426 EIA IHC (p) WB Goat Internal Region Log in to see Polyclonal 0
1 ABIN116519 EIA IP WB Rabbit Log in to see Polyclonal 2
1 ABIN318090 EIA WB Rabbit pSer10 Log in to see Polyclonal 0
1 ABIN318813 EIA IP WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN1106656 EIA WB Mouse IgG1 Log in to see 3D8 0

General

Antigen Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
Epitope pThr198
(82), (55), (47), (42), (35), (28), (24), (20), (19), (19), (19), (19), (17), (16), (14), (13), (13), (10), (9), (8), (7), (7), (6), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(789), (439), (375), (182), (42), (24), (18), (8), (8), (7), (3), (3), (2), (1), (1), (1)
Host Rabbit
(480), (307), (37), (2), (2), (1)
Clonality
Polyclonal
Conjugate This CDKN1B antibody is un-conjugated
(41), (36), (35), (34), (34), (29), (9), (8), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA)
(629), (338), (243), (233), (201), (198), (101), (98), (84), (55), (39), (28), (13), (7), (5), (3), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CDKN1B Antibody

Target Details CDKN1B Application Details Handling References for anti-CDKN1B antibody (ABIN358459) Images
Specificity This antibody detects p27Kip1 pThr198.
Purification Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T198 of human 27Kip1.
Isotype Ig Fraction
Plasmids, Primers & others

Target Details CDKN1B

Product Details anti-CDKN1B Antibody Application Details Handling References for anti-CDKN1B antibody (ABIN358459) Images back to top
Antigen
Alternative Name CDKN1B / KIP1 (CDKN1B Antibody Abstract)
Background P27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. This protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.Synonyms: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1
Molecular Weight 22073 Da
Gene ID 1027, 9606
UniProt P46527
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy

Application Details

Product Details anti-CDKN1B Antibody Target Details CDKN1B Handling References for anti-CDKN1B antibody (ABIN358459) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details References for anti-CDKN1B antibody (ABIN358459) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-CDKN1B antibody (ABIN358459)

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details Handling Images back to top
Background publications

Mogensen, Shaw: "Current approaches to unravel the mystery of dilated cardiomyopathy, a common cause of hereditary heart failure." in: Expert review of proteomics, Vol. 2, Issue 6, pp. 827-30, 2005

Kawamata, Inagaki, Mizumura, Sugimoto, Sakajiri, Ohyanagi-Hara, Oshimi: "Methylation status analysis of cell cycle regulatory genes (p16INK4A, p15INK4B, p21Waf1/Cip1, p27Kip1 and p73) in natural killer cell disorders." in: European journal of haematology, Vol. 74, Issue 5, pp. 424-9, 2005

Andreu, Lledó, Poch, Ivorra, Albero, Martínez-Climent, Montiel-Duarte, Rifón, Pérez-Calvo, Arbona, Prósper, Pérez-Roger: "BCR-ABL induces the expression of Skp2 through the PI3K pathway to promote p27Kip1 degradation and proliferation of chronic myelogenous leukemia cells." in: Cancer research, Vol. 65, Issue 8, pp. 3264-72, 2005

Wingate, Zhang, McGarhen, Bedrosian, Harper, Keyomarsi: "The tumor-specific hyperactive forms of cyclin E are resistant to inhibition by p21 and p27." in: The Journal of biological chemistry, Vol. 280, Issue 15, pp. 15148-57, 2005

Wang, Hou, Mohapatra, Ma, Cress, Pledger, Chen: "Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12339-43, 2005

Images

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details Handling References for anti-CDKN1B antibody (ABIN358459) back to top
Supplier Images
 image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pThr198) antibody (ABIN358459) anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pThr198) antibody