anti-Human CDKN1B antibody for Immunohistochemistry

Recommended CDKN1B Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
  • CDKN4
  • KIP1
  • MEN1B
  • MEN4
  • P27KIP1
  • cdkn1b
  • sb:cb611
  • wu:fb63g10
  • wu:fb64g10
  • wu:fe18e03
  • CDKN1B
  • Kip1
  • p27
  • Cdki1b
  • AA408329
  • AI843786
  • p27Kip1
  • cdkn1bl
  • cyclin dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Bb
  • cyclin-dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Ba
  • CDKN1B
  • cdkn1bb
  • Cdkn1b
  • cdkn1ba
Epitope
AA 1-199
82
55
47
42
36
28
24
20
19
19
19
19
17
16
14
13
13
10
9
8
7
7
6
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
789
439
375
182
42
24
18
8
8
7
3
3
2
1
1
1
Host
Mouse
481
306
37
2
2
1
Clonality
Monoclonal
Conjugate
This CDKN1B antibody is un-conjugated
41
36
35
34
34
29
9
8
8
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
Application
ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
628
337
243
232
201
197
101
98
84
55
39
28
14
7
5
3
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN4948200
$ 461.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.404629 ABIN2705080 IHC WB Rabbit C-Term Log in to see Polyclonal 0
8.404629 ABIN2705081 IC IF IHC WB Rabbit C-Term, pSer187 Log in to see Polyclonal 0
8.404629 ABIN6271268 ELISA ICC IF IHC WB Rabbit IgG pThr157 Log in to see Polyclonal 0
8.404629 ABIN6256493 ELISA ICC IF IHC WB Rabbit IgG pThr187 Log in to see Polyclonal 0
8.404629 ABIN6271270 ELISA ICC IF IHC WB Rabbit IgG pSer178 Log in to see Polyclonal 0
8.404629 ABIN1532962 IHC ELISA WB Rabbit IgG AA 1-50 Log in to see Polyclonal 0
8.404629 ABIN489992 ELISA IHC IHC (p) WB Goat AA 153-165 Log in to see Polyclonal 0
8.404629 ABIN337229 IHC IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 0
8.404629 ABIN481755 IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
8.404629 ABIN214041 IHC IHC (p) WB Rabbit IgG Ser10 Log in to see Polyclonal 0
8.404629 ABIN609774 IHC IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
8.404629 ABIN4342483 IHC IHC (p) WB Rabbit Log in to see Polyclonal 1
8.404629 ABIN626030 ELISA IF IHC IHC (p) WB Mouse IgG1, kappa AA 1-199 Log in to see 4B4-E6 0
8.404629 ABIN1870060 IHC WB Rabbit IgG pThr187 Log in to see Polyclonal 0
8.404629 ABIN2469670 IHC WB Rabbit IgG Ser10 Log in to see Polyclonal 0
8.404629 ABIN2470648 IP IHC WB Rabbit C-Term Log in to see Polyclonal 0
8.404629 ABIN3030631 DB IHC ELISA Rabbit Ig Fraction pThr157 Log in to see Polyclonal 0
8.404629 ABIN2970966 IHC WB Rabbit IgG pThr187 Log in to see Polyclonal 0
8.404629 ABIN3001772 IHC WB Rabbit IgG pSer178 Log in to see Polyclonal 0
8.404629 ABIN2970964 IHC WB Rabbit IgG pSer10 Log in to see Polyclonal 0

General

Antigen Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
Epitope AA 1-199
(82), (55), (47), (42), (36), (28), (24), (20), (19), (19), (19), (19), (17), (16), (14), (13), (13), (10), (9), (8), (7), (7), (6), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(789), (439), (375), (182), (42), (24), (18), (8), (8), (7), (3), (3), (2), (1), (1), (1)
Host Mouse
(481), (306), (37), (2), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CDKN1B antibody is un-conjugated
(41), (36), (35), (34), (34), (29), (9), (8), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(628), (337), (243), (232), (201), (197), (101), (98), (84), (55), (39), (28), (14), (7), (5), (3), (1), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-CDKN1B Antibody

Target Details CDKN1B Application Details Handling References for anti-CDKN1B antibody (ABIN4948200) Images
Specificity CDKN1B (AAH01971, 1 a.a. ~ 199 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: CDKN1B (AAH01971, 1 a.a. ~ 198 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 4B4-E6
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details CDKN1B

Product Details anti-CDKN1B Antibody Application Details Handling References for anti-CDKN1B antibody (ABIN4948200) Images back to top
Antigen
Alternative Name CDKN1B (CDKN1B Antibody Abstract)
Background

Other Names: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1

Gene Description: cyclin-dependent kinase inhibitor 1B (p27, Kip1)

Gene Summary: This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq

Molecular Weight 22073 DA
Gene ID 1027
NCBI Accession NP_004055
UniProt P46527
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy

Application Details

Product Details anti-CDKN1B Antibody Target Details CDKN1B Handling References for anti-CDKN1B antibody (ABIN4948200) Images back to top
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Restrictions For Research Use only

Handling

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details References for anti-CDKN1B antibody (ABIN4948200) Images back to top
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-CDKN1B antibody (ABIN4948200)

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details Handling Images back to top
Background publications

Qin, Wang, Wang, Ma, Ni, Ke: "JAB1 expression is associated with inverse expression of p27(kip1) in hepatocellular carcinoma." in: Hepato-gastroenterology, Vol. 57, Issue 99-100, pp. 547-53, 2010

Canbay, Eraltan, Cercel, Isbir, Gazioglu, Aydogan, Cacina, Cengiz, Ferahman, Zengin, Unal: "CCND1 and CDKN1B polymorphisms and risk of breast cancer." in: Anticancer research, Vol. 30, Issue 7, pp. 3093-8, 2010

Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010

Wang, Ni, Li, Wei, Zhu, Wang, Cao, Ma: "Analysis of cyclin-dependent kinase inhibitor 1B mutation in Han Chinese women with premature ovarian failure." in: Reproductive biomedicine online, Vol. 21, Issue 2, pp. 212-4, 2010

Molatore, Pellegata: "The MENX syndrome and p27: relationships with multiple endocrine neoplasia." in: Progress in brain research, Vol. 182, pp. 295-320, 2010

Images

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details Handling References for anti-CDKN1B antibody (ABIN4948200) back to top
Supplier Images
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-199) antibody (ABIN4948200) Immunofluorescence of monoclonal antibody to CDKN1B on HeLa cell (antibody concentrat...
Immunohistochemistry (IHC) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-199) antibody (ABIN4948200) anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-199) antibody (Image 2)
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-199) antibody (ABIN4948200) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-199) antibody (ABIN4948200) Western Blot analysis of CDKN1B expression in transfected 293T cell line by CDKN1B mo...
Immunohistochemistry (IHC) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-199) antibody (ABIN4948200) Immunoperoxidase of monoclonal antibody to CDKN1B on formalin-fixed paraffin-embedded...
Immunofluorescence (IF) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-199) antibody (ABIN4948200) Immunofluorescence of monoclonal antibody to CDKN1B on HeLa cell. [antibody concentra...
ELISA image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-199) antibody (ABIN4948200) Detection limit for recombinant GST tagged CDKN1B is 10 ng/ml as a capture antibody.
 image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-199) antibody (ABIN4948200) Proximity Ligation Analysis of protein-protein interactions between AKT1 and CDKN1B H...