anti-Human CDKN1B antibody for Immunoprecipitation

Recommended CDKN1B Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
  • CDKN4
  • KIP1
  • MEN1B
  • MEN4
  • P27KIP1
  • cdkn1b
  • sb:cb611
  • wu:fb63g10
  • wu:fb64g10
  • wu:fe18e03
  • CDKN1B
  • Kip1
  • p27
  • Cdki1b
  • AA408329
  • AI843786
  • p27Kip1
  • cdkn1bl
  • cyclin dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Bb
  • cyclin-dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Ba
  • CDKN1B
  • cdkn1bb
  • Cdkn1b
  • cdkn1ba
Epitope
Center
81
56
47
37
29
29
25
20
19
19
19
19
17
16
14
13
10
9
8
7
7
6
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
779
423
359
182
40
25
19
8
8
7
3
3
2
1
1
1
Host
Rabbit
470
307
39
2
2
1
Clonality
Polyclonal
Conjugate
This CDKN1B antibody is un-conjugated
40
35
35
34
33
29
9
7
7
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
629
338
241
231
199
196
102
92
84
52
37
17
14
5
5
3
1
1
1
Options
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Catalog No. ABIN2854753
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19.08413 ABIN5596806 ELISA IHC IP WB Rabbit IgG Internal Region, pSer140 Log in to see Polyclonal 0
17.5 ABIN967391 BI IP WB Mouse IgG1 Log in to see G173-524 6
16.08413 ABIN184587 ELISA IP WB Goat C-Term Log in to see Polyclonal 1
15.940398 ABIN442186 ICC IF IHC IHC (fro) IHC (p) IP WB Rabbit IgG Center Log in to see Polyclonal 0
13.08413 ABIN6138338 FACS ICC IHC IP WB Rabbit Log in to see Polyclonal 0
7 ABIN337229 IHC IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 0
7 ABIN1106655 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
7 ABIN609774 IHC IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
6.5380793 ABIN5949261 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG AA 150-250 Log in to see SU37-04 0
4 ABIN116519 EIA IP WB Rabbit Log in to see Polyclonal 2
4 ABIN783901 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
4 ABIN2737293 ICC FACS IP IHC WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN290297 IP ELISA WB Rabbit Log in to see Polyclonal 0
4 ABIN5575102 ELISA IP WB Rabbit Log in to see Polyclonal 0
4 ABIN1732934 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
4 ABIN3059735 FACS IF/ICC IHC IP WB Rabbit IgG Log in to see Polyclonal 0
2.0380793 ABIN269609 IP ELISA WB Rabbit Log in to see Polyclonal 3
1 ABIN600048 IHC IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN199528 FACS IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 AA 83-204 Log in to see 0
1 ABIN1501971 IP Sepharose Mouse IgG2a Log in to see IMD-27 0

General

Antigen Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
Epitope Center
(81), (56), (47), (37), (29), (29), (25), (20), (19), (19), (19), (19), (17), (16), (14), (13), (10), (9), (8), (7), (7), (6), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(779), (423), (359), (182), (40), (25), (19), (8), (8), (7), (3), (3), (2), (1), (1), (1)
Host Rabbit
(470), (307), (39), (2), (2), (1)
Clonality
Polyclonal
Conjugate This CDKN1B antibody is un-conjugated
(40), (35), (35), (34), (33), (29), (9), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(629), (338), (241), (231), (199), (196), (102), (92), (84), (52), (37), (17), (14), (5), (5), (3), (1), (1), (1)
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Product Details anti-CDKN1B Antibody

Target Details CDKN1B Application Details Handling Images
Characteristics Rabbit Polyclonal antibody to p27 Kip1 (cyclin-dependent kinase inhibitor 1B (p27, Kip1))
p27 Kip1 antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human p27 Kip1. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details CDKN1B

Product Details anti-CDKN1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name p27 Kip1 (CDKN1B Antibody Abstract)
Background This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Cellular Localization: Nucleus , Cytoplasm
Molecular Weight 22 kDa
Gene ID 1027
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy

Application Details

Product Details anti-CDKN1B Antibody Target Details CDKN1B Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: MCF-7 , NIH-3T3 , CCRF-SB , MFB (mouse fibroblast) , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Restrictions For Research Use only

Handling

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details Images back to top
Format Liquid
Concentration 0.96 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) WB Image p27 Kip1 antibody detects p27 Kip1 protein by western blot analysis. Whole c...
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) WB Image Sample (50 ug of whole cell lysate) A: CCRF-SB 12% SDS PAGE antibody diluted...
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) WB Image Sample (50 ug of whole cell lysate) A: MFB (mouse fibroblast) 12% SDS PAGE a...
Immunoprecipitation (IP) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) IP Image Immunoprecipitation of p27 Kip1 protein from MCF-7 whole cell extracts using...
Immunofluorescence (IF) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) ICC/IF Image p27 Kip1 antibody detects p27 Kip1 protein at cytoplasm and nucleus by i...
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) WB Image p27 Kip1 antibody detects p27 Kip1 protein by western blot analysis. Various...