anti-Monkey CDKN1B antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended CDKN1B Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
  • CDKN4
  • KIP1
  • MEN1B
  • MEN4
  • P27KIP1
  • cdkn1b
  • sb:cb611
  • wu:fb63g10
  • wu:fb64g10
  • wu:fe18e03
  • CDKN1B
  • Kip1
  • p27
  • Cdki1b
  • AA408329
  • AI843786
  • p27Kip1
  • cdkn1bl
  • cyclin dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Bb
  • cyclin-dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Ba
  • CDKN1B
  • cdkn1bb
  • Cdkn1b
  • cdkn1ba
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
786
428
363
181
40
25
19
8
8
7
3
3
2
1
1
1
Host
Mouse
479
305
39
2
2
1
Clonality
Monoclonal
Conjugate
This CDKN1B antibody is conjugated to CF405M
40
35
35
34
33
29
9
7
7
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
634
338
241
237
200
197
101
94
83
54
37
17
13
5
5
3
1
1
1
Options
Supplier
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Supplier Product No.
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Catalog No. ABIN6174300
$ 209.23
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6174312 FACS IF IHC (f) WB CF®568 Mouse IgG Log in to see DCS-72-F6 KIP1-769 0
1 ABIN6174340 FACS IF IHC (f) WB CF®640R Mouse IgG1, kappa Log in to see DCS-72-F6 0
1 ABIN6174348 FACS IF IHC (f) WB CF®660R Mouse IgG1, kappa Log in to see DCS-72-F6 0
1 ABIN6174354 FACS IF IHC (f) WB CF®680 Mouse IgG1, kappa Log in to see DCS-72-F6 0
1 ABIN6174360 FACS IF IHC (f) WB CF®594 Mouse IgG1, kappa Log in to see DCS-72-F6 0
1 ABIN6174390 FACS IF IHC (f) WB CF®770 Mouse IgG1, kappa Log in to see KIP1-769 0
1 ABIN6174394 FACS IF IHC (f) WB CF®680R Mouse IgG1, kappa Log in to see KIP1-769 0
1 ABIN6174412 FACS IF IHC (f) WB CF®405S Mouse IgG1, kappa Log in to see SX53G8 0
1 ABIN6174420 FACS IF IHC (f) WB CF®647 Mouse IgG1, kappa Log in to see SX53G8 0
1 ABIN6174426 FACS IF IHC (f) WB CF®568 Mouse IgG1, kappa Log in to see SX53G8 0
1 ABIN6174432 FACS IF IHC (f) WB CF®680R Mouse IgG1, kappa Log in to see SX53G8 0
1 ABIN6174336 FACS IF IHC (f) WB CF®405S Mouse IgG1, kappa Log in to see DCS-72-F6 0
1 ABIN6174350 FACS IF IHC (f) WB CF®568 Mouse IgG1, kappa Log in to see DCS-72-F6 0
1 ABIN6174392 FACS IF IHC (f) WB CF®680 Mouse IgG1, kappa Log in to see KIP1-769 0
1 ABIN6174424 FACS IF IHC (f) WB CF®660R Mouse IgG1, kappa Log in to see SX53G8 0
1 ABIN6174428 FACS IF IHC (f) WB CF®770 Mouse IgG1, kappa Log in to see SX53G8 0
1 ABIN6174306 FACS IF IHC (f) WB CF®647 Mouse IgG Log in to see DCS-72-F6 KIP1-769 0
1 ABIN6174308 FACS IF IHC (f) WB CF®555 Mouse IgG Log in to see DCS-72-F6 KIP1-769 0
1 ABIN6174302 FACS IF IHC (f) WB CF®640R Mouse IgG Log in to see DCS-72-F6 KIP1-769 0
1 ABIN6174322 FACS IF IHC (f) WB CF®594 Mouse IgG Log in to see DCS-72-F6 KIP1-769 0

General

Antigen Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(786), (428), (363), (181), (40), (25), (19), (8), (8), (7), (3), (3), (2), (1), (1), (1)
Host Mouse
(479), (305), (39), (2), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CDKN1B antibody is conjugated to CF405M
(40), (35), (35), (34), (33), (29), (9), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
(634), (338), (241), (237), (200), (197), (101), (94), (83), (54), (37), (17), (13), (5), (5), (3), (1), (1), (1)
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Product Details anti-CDKN1B Antibody

Target Details CDKN1B Application Details Handling Images
Purpose Mouse Monoclonal anti-p27 / KIP1 (DCS-72.F6 + KIP1/769 (SX53G8)), CF405M Conjugate
Characteristics This antibody recognizes a 27 kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Mouse recombinant p27 protein (DCS-72.F6), Recombinant human CDKN1B protein (KIP1/769)
Clone DCS-72-F6 KIP1-769
Isotype IgG
Plasmids, Primers & others

Target Details CDKN1B

Product Details anti-CDKN1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name p27 / KIP1 (CDKN1B Antibody Abstract)
Background CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4
Molecular Weight 25-26 kDa
Gene ID 1027, 238990
UniProt P46527
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy

Application Details

Product Details anti-CDKN1B Antibody Target Details CDKN1B Handling Images back to top
Application Notes Immunohistology formalin-fixed 0.25-0.5 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Immunofluorescence 0.5-1 μg/mL
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Western blotting 0.5-1 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa or MCF7 cells. Tonsil, Breast, Cervical or Colon Carcinoma.

Restrictions For Research Use only

Handling

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light