|Antigen||Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CDKN1B antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-CDKN1B AntibodyTarget Details CDKN1B Application Details Handling Images
|Specificity||This antibody detects P27Kip1 / CDKN1B at C-term.|
Species reactivity (expected):Mouse, Rat, Cow, Canine.
Species reactivity (tested):Human.
|Purification||Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide|
|Immunogen||Peptide from C Terminus of the protein sequence according to NP_004055.1 Genename: CDKN1B|
|Plasmids, Primers & others|
Target Details CDKN1BProduct Details anti-CDKN1B Antibody Application Details Handling Images back to top
|Alternative Name||CDKN1B / KIP1 (CDKN1B Antibody Abstract)|
|Background||Cell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4. p27 Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27 Kip 1, while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.Synonyms: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1|
|Pathways||Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy|
Application DetailsProduct Details anti-CDKN1B Antibody Target Details CDKN1B Handling Images back to top
Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 26 kDa band observed in Human Breast and Prostatelysates (calculated MW of 22.6 kDa according to NP_004055.1). The observed molecularweight corresponds to earlier findings in literature with different antibodies (Xia et al, ProcNatl Acad Sci U S A.2005 Sep 27,102(39): 14028-33, PMID: 16169901).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CDKN1B Antibody Target Details CDKN1B Application Details Images back to top
|Buffer||Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|