anti-Human E2F4 antibody for Immunohistochemistry

Recommended E2F4 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 4, P107/p130-Binding (E2F4) Antibodies
  • E2F-4
  • 2010111M04Rik
  • AI427446
  • E2F4
  • e2f-4
  • fb72f07
  • wu:fb72f07
  • wu:fe05f06
  • zgc:63815
  • E2F transcription factor 4
  • E2F transcription factor 4 S homeolog
  • E2F4
  • E2f4
  • e2f4.S
  • e2f4
Epitope
C-Term
9
4
4
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
94
41
39
6
5
5
5
4
4
4
2
1
1
Host
Rabbit
55
25
17
Clonality
Polyclonal
Conjugate
This E2F4 antibody is un-conjugated
5
4
3
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
85
62
31
17
11
11
4
2
2
Options
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Catalog No. ABIN2777218
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.933557 ABIN2706063 IHC WB Rabbit Center Log in to see Polyclonal 0
8.933557 ABIN1533259 IHC ELISA WB Rabbit IgG AA 51-100 Log in to see Polyclonal 0
8.933557 ABIN214109 IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
8.933557 ABIN6261411 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
8.933557 ABIN1848516 IHC ELISA WB Rabbit Internal Region Log in to see Polyclonal 0
8.933557 ABIN2425787 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
1 ABIN439334 ICC IF IHC IHC (p) IP PLA WB Rabbit AA 363-413 Log in to see Polyclonal 1
1 ABIN384006 GS IF IHC IHC (p) WB Mouse IgG1 Log in to see 4E2F04 0
1 ABIN1821565 IHC WB Mouse IgG1, kappa Log in to see GF1 0
1 ABIN2891380 IHC IHC (p) WB Rabbit Leu50, Leu83 Log in to see Polyclonal 0
1 ABIN2599370 IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 0
1 ABIN1978607 IHC IHC (p) IP PLA WB Rabbit IgG AA 363-413 Log in to see Polyclonal 0
1 ABIN1978608 IF IHC IHC (p) Rabbit IgG AA 363-413 Log in to see Polyclonal 0
1 ABIN2256953 IF IHC WB Mouse IgG1 Log in to see 3F377 0
1 ABIN2256949 IHC WB Mouse IgG1 kappa Log in to see 13A177 0
1 ABIN384009 GS IF IHC IHC (p) WB Biotin Mouse IgG1 Log in to see 4E2F04 0
1 ABIN5700805 ELISA IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5619526 IHC WB Rabbit Center Log in to see Polyclonal 0
1 ABIN1843408 IHC WB Rabbit Log in to see Polyclonal 0
1 ABIN6107147 ELISA IHC Rabbit IgG AA 160-299 Log in to see Polyclonal 0

General

Antigen E2F Transcription Factor 4, P107/p130-Binding (E2F4) Antibodies
Epitope C-Term
(9), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(94), (41), (39), (6), (5), (5), (5), (4), (4), (4), (2), (1), (1)
Host Rabbit
(55), (25), (17)
Clonality
Polyclonal
Conjugate This E2F4 antibody is un-conjugated
(5), (4), (3), (2), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(85), (62), (31), (17), (11), (11), (4), (2), (2)
Pubmed 1 reference available
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Product Details anti-E2F4 Antibody

Target Details E2F4 Application Details Handling References for anti-E2F4 antibody (ABIN2777218) Images
Blocking Peptide Blocking peptide for this product available: ABIN976081
Sequence SSELLEELMS SEVFAPLLRL SPPPGDHDYI YNLDESEGVC DLFDVPVLNL
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%
Characteristics This is a rabbit polyclonal antibody against E2F4. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human E2F4
Plasmids, Primers & others

Target Details E2F4

Product Details anti-E2F4 Antibody Application Details Handling References for anti-E2F4 antibody (ABIN2777218) Images back to top
Antigen
Alternative Name E2F4 (E2F4 Antibody Abstract)
Background The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins. It is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It also plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.
Alias Symbols: E2F-4
Protein Interaction Partner: RBL2, TFDP1, RBL1, RB1, XPO1, PCM1, HDAC1, POLR1D, CHMP2A, AP3S2, TRIM28, LIN9, ARID3A, NCOR2, HDAC3, AR, CEBPA, LIN54, LIN37, UBC, ATAD2, SIN3A, ASH2L, HCFC1, TRRAP, KAT2A, ID2, E2F1, KMT2A, ID3, SMAD3, NOLC1, TFDP2, NDN, SMAD2, BRCA1, RYBP, RNF144A, FHL
Protein Size: 413
Molecular Weight 44 kDa
Gene ID 1874
NCBI Accession NM_001950, NP_001941
UniProt Q16254
Pathways Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size

Application Details

Product Details anti-E2F4 Antibody Target Details E2F4 Handling References for anti-E2F4 antibody (ABIN2777218) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 413 AA

Restrictions For Research Use only

Handling

Product Details anti-E2F4 Antibody Target Details E2F4 Application Details References for anti-E2F4 antibody (ABIN2777218) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-E2F4 antibody (ABIN2777218)

Product Details anti-E2F4 Antibody Target Details E2F4 Application Details Handling Images back to top
Background publications

DuPree, Mazumder, Almasan: "Genotoxic stress induces expression of E2F4, leading to its association with p130 in prostate carcinoma cells." in: Cancer research, Vol. 64, Issue 13, pp. 4390-3, 2004

Images

Product Details anti-E2F4 Antibody Target Details E2F4 Application Details Handling References for anti-E2F4 antibody (ABIN2777218) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-E2F Transcription Factor 4, P107/p130-Binding (E2F4) (C-Term) antibody (ABIN2777218) Human Intestine
Immunohistochemistry (IHC) image for anti-E2F Transcription Factor 4, P107/p130-Binding (E2F4) (C-Term) antibody (ABIN2777218) Human Stomach
Western Blotting (WB) image for anti-E2F Transcription Factor 4, P107/p130-Binding (E2F4) (C-Term) antibody (ABIN2777218) WB Suggested Anti-E2F4 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive...