anti-Human E2F4 antibody for Immunohistochemistry

Recommended E2F4 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 4, P107/p130-Binding (E2F4) Antibodies
  • E2F-4
  • 2010111M04Rik
  • AI427446
  • E2F4
  • e2f-4
  • fb72f07
  • wu:fb72f07
  • wu:fe05f06
  • zgc:63815
  • E2F transcription factor 4
  • E2F transcription factor 4 S homeolog
  • E2F4
  • E2f4
  • e2f4.S
  • e2f4
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
93
41
39
7
6
6
5
5
5
3
3
2
1
Host
Rabbit
55
25
16
Clonality
Polyclonal
Conjugate
This E2F4 antibody is un-conjugated
5
4
3
2
2
2
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
84
61
31
17
11
10
3
2
2
Supplier
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Available images

Catalog No. ABIN6261411
€ 345.81
Plus shipping costs €45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.25679 ABIN5619526 IHC WB Rabbit Center Log in to see Polyclonal 0
13.25679 ABIN2139560 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
13.25679 ABIN2139559 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
13.132549 ABIN2777218 IHC WB Rabbit C-Term Log in to see Polyclonal 1
13.132549 ABIN1848516 IHC ELISA WB Rabbit Internal Region Log in to see Polyclonal 0
11.47553 ABIN439334 ICC IF IHC IHC (p) IP PLA WB Rabbit AA 363-413 Log in to see Polyclonal 1
10.132549 ABIN2706063 IHC WB Rabbit Center Log in to see Polyclonal 0
10.132549 ABIN1533259 IHC ELISA WB Rabbit IgG AA 51-100 Log in to see Polyclonal 0
10.132549 ABIN214109 IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
10.132549 ABIN1576163 IHC ELISA WB Rabbit Log in to see Polyclonal 0
10.132549 ABIN2425787 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
4 ABIN384006 GS IF IHC IHC (p) WB Mouse IgG1 Log in to see 4E2F04 0
1 ABIN1821565 IHC WB Mouse IgG1, kappa Log in to see GF1 0
1 ABIN2891380 IHC IHC (p) WB Rabbit Leu50, Leu83 Log in to see Polyclonal 0
1 ABIN2599370 IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 0
1 ABIN1978607 IHC IHC (p) IP PLA WB Rabbit IgG AA 363-413 Log in to see Polyclonal 0
1 ABIN1978608 IF IHC IHC (p) Rabbit IgG AA 363-413 Log in to see Polyclonal 0
1 ABIN2256949 IHC WB Mouse IgG1 kappa Log in to see 13A177 0
1 ABIN2256953 IF IHC WB Mouse IgG1 Log in to see 3F377 0
1 ABIN384009 GS IF IHC IHC (p) WB Biotin Mouse IgG1 Log in to see 4E2F04 0

General

Antigen E2F Transcription Factor 4, P107/p130-Binding (E2F4) Antibodies
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(93), (41), (39), (7), (6), (6), (5), (5), (5), (3), (3), (2), (1)
Host Rabbit
(55), (25), (16)
Clonality
Polyclonal
Conjugate This E2F4 antibody is un-conjugated
(5), (4), (3), (2), (2), (2)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(84), (61), (31), (17), (11), (10), (3), (2), (2)
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Product Details anti-E2F4 Antibody

Target Details E2F4 Application Details Handling Images
Specificity E2F4 antibody detects endogenous levels of total E2F4
Cross-Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthesized peptide derived from human E2F4
Isotype IgG
Plasmids, Primers & others

Target Details E2F4

Product Details anti-E2F4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name E2F4 (E2F4 Antibody Abstract)
Background

Description: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.

Gene: E2F4

Molecular Weight 44kDa
Gene ID 1874
UniProt Q16254, Q15329
Pathways Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size

Application Details

Product Details anti-E2F4 Antibody Target Details E2F4 Handling Images back to top
Application Notes WB: 1:500~1:3000 IHC: 1:50~1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-E2F4 Antibody Target Details E2F4 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-E2F4 Antibody Target Details E2F4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-E2F Transcription Factor 4, P107/p130-Binding (E2F4) antibody (ABIN6261411) Western blot analysis of extracts from HepG2, using E2F4 Antibody. Lane 1 was treated...
Western Blotting (WB) image for anti-E2F Transcription Factor 4, P107/p130-Binding (E2F4) antibody (ABIN6261411) Western blot analysis on COS7 cell lysate using E2F4 Antibody,The lane on the left is...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-E2F Transcription Factor 4, P107/p130-Binding (E2F4) antibody (ABIN6261411) ABIN6266531 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized...
Immunohistochemistry (IHC) image for anti-E2F Transcription Factor 4, P107/p130-Binding (E2F4) antibody (ABIN6261411) ABIN6266531 at 1/100 staining human brain tissue sections by IHC-P. The tissue was fo...