anti-Rat (Rattus) SMAD3 antibody for Immunohistochemistry

Recommended SMAD3 Antibody (supplied by: Log in to see )

Antigen
SMAD, Mothers Against DPP Homolog 3 (SMAD3) Antibodies
  • madh3
  • madh3a
  • smad3
  • wu:fa99e03
  • HSPC193
  • HsT17436
  • JV15-2
  • LDS1C
  • LDS3
  • MADH3
  • XSmad3
  • XenMLP
  • Xmad3
  • madh3-A
  • Madh3
  • AU022421
  • Smad 3
  • mad3
  • SMAD family member 3
  • SMAD family member 3a
  • SMAD family member 3 L homeolog
  • SMAD3
  • smad3a
  • smad3.L
  • Smad3
Epitope
N-Term
74
63
53
50
39
36
23
20
18
18
18
16
12
12
11
11
9
9
8
7
7
6
5
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Guinea Pig, Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
610
336
321
57
48
42
38
32
23
18
18
4
3
3
2
1
1
1
1
1
1
Host
Rabbit
562
63
8
Clonality
Polyclonal
Conjugate
This SMAD3 antibody is un-conjugated
24
22
20
12
12
11
7
7
6
6
6
5
5
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
489
270
265
131
118
68
50
43
31
19
18
9
7
2
2
1
1
Options
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Catalog No. ABIN2792537
$ 229.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.856452 ABIN2707070 IHC WB Rabbit Center Log in to see Polyclonal 0
8.856452 ABIN2707071 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
8.856452 ABIN2707068 IHC WB Rabbit pSer179 Log in to see Polyclonal 0
8.856452 ABIN4354683 ELISA FACS IHC IHC (p) WB Rabbit pSer423, pSer425 Log in to see Polyclonal 2
8.856452 ABIN1531631 IHC ELISA WB Rabbit IgG AA 145-194, pThr179 Log in to see Polyclonal 1
8.856452 ABIN1532654 IHC ELISA WB Rabbit IgG AA 170-219 Log in to see Polyclonal 1
8.856452 ABIN6265173 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 2
8.856452 ABIN1531633 IHC ELISA WB Rabbit IgG AA 174-223, pSer208 Log in to see Polyclonal 0
8.856452 ABIN1531200 IHC ELISA WB Rabbit IgG AA 376-425, pSer425 Log in to see Polyclonal 0
8.856452 ABIN1532216 IHC ELISA WB Rabbit IgG AA 376-425 Log in to see Polyclonal 0
8.856452 ABIN6256335 ELISA ICC IF IHC WB Rabbit IgG pSer425 Log in to see Polyclonal 1
8.856452 ABIN626100 ELISA IHC IHC (p) WB Mouse IgG1, kappa AA 120-222 Log in to see 7F3 0
8.856452 ABIN214557 ELISA FACS IF IHC IHC (p) WB Rabbit Ser425, pSer423 Log in to see Polyclonal 0
8.856452 ABIN296130 IHC IHC (p) WB Rabbit AA 100-150 Log in to see Polyclonal 0
8.856452 ABIN337277 ELISA IHC IHC (p) IP WB Mouse IgG1, kappa Log in to see AF9F7 0
8.856452 ABIN3188041 IHC WB Rabbit IgG Log in to see Polyclonal 0
8.856452 ABIN3181632 IHC WB Mouse IgG1 Log in to see 8B5 0
8.856452 ABIN3181648 IHC Mouse IgG1 Log in to see 6F7 0
8.856452 ABIN2852667 IHC WB Mouse Log in to see 0
8.856452 ABIN6255085 ELISA ICC IF IHC WB Rabbit IgG pSer208 Log in to see Polyclonal 0

General

Antigen SMAD, Mothers Against DPP Homolog 3 (SMAD3) Antibodies
Epitope N-Term
(74), (63), (53), (50), (39), (36), (23), (20), (18), (18), (18), (16), (12), (12), (11), (11), (9), (9), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Guinea Pig, Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
(610), (336), (321), (57), (48), (42), (38), (32), (23), (18), (18), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(562), (63), (8)
Clonality
Polyclonal
Conjugate This SMAD3 antibody is un-conjugated
(24), (22), (20), (12), (12), (11), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(489), (270), (265), (131), (118), (68), (50), (43), (31), (19), (18), (9), (7), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-SMAD3 Antibody

Target Details SMAD3 Application Details Handling References for anti-SMAD3 antibody (ABIN2792537) Images
Sequence FTPPIVKRLL GWKKGEQNGQ EEKWCEKAVK SLVKKLKKTG QLDELEKAIT
Cross-Reactivity Dog (Canine), Chicken, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
Predicted Reactivity Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%
Characteristics This is a rabbit polyclonal antibody against SMAD3. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD3
Plasmids, Primers & others

Target Details SMAD3

Product Details anti-SMAD3 Antibody Application Details Handling References for anti-SMAD3 antibody (ABIN2792537) Images back to top
Antigen
Alternative Name SMAD3 (SMAD3 Antibody Abstract)
Background SMAD3 is a member of Smad family proteins. SMAD family is known to be important cytoplasmic mediators of signals from the transforming growth factor-beta (TGF-beta) receptor serine/threonine kinases.
Alias Symbols: LDS1C, MADH3, JV15-2, HSPC193, HsT17436
Protein Interaction Partner: TEKT4, CCDC33, CPSF7, MEOX2, HDAC1, PHC2, WWP1, AES, BLZF1, UBC, TP53, SMAD4, BACH1, MAFK, FOXO3, NEDD8, TRIM33, WWP2, SMURF2, FOXM1, TRIM62, TP63, OTUB1, NEDD4L, BAG3, TGFB1I1, CSNK1D, RUNX1, HCVgp1, TSC2, FHL3, FHL2, FHL1, Melk, IRF7, RNF111, LCK, KLF5,
Protein Size: 425
Molecular Weight 48 kDa
Gene ID 4088
NCBI Accession NM_005902, NP_005893
UniProt Q92940
Pathways Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy

Application Details

Product Details anti-SMAD3 Antibody Target Details SMAD3 Handling References for anti-SMAD3 antibody (ABIN2792537) Images back to top
Application Notes WB Suggested Anti-SMAD3 Antibody Titration: 2.0 µg/mL
Positive Control: Human Liver.
Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 425 AA

Restrictions For Research Use only

Handling

Product Details anti-SMAD3 Antibody Target Details SMAD3 Application Details References for anti-SMAD3 antibody (ABIN2792537) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SMAD3 antibody (ABIN2792537)

Product Details anti-SMAD3 Antibody Target Details SMAD3 Application Details Handling Images back to top
Background publications

Cheng, Chang, Chao, Lee: "Hepatitis C viral proteins interact with Smad3 and differentially regulate TGF-beta/Smad3-mediated transcriptional activation." in: Oncogene, Vol. 23, Issue 47, pp. 7821-38, 2004

Images

Product Details anti-SMAD3 Antibody Target Details SMAD3 Application Details Handling References for anti-SMAD3 antibody (ABIN2792537) back to top
Supplier Images
Western Blotting (WB) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (N-Term) antibody (ABIN2792537) WB Suggested Anti-SMAD3 Antibody Titration: 2.0ug/ml Positive Control: Human Liver
Immunohistochemistry (IHC) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (N-Term) antibody (ABIN2792537) Human Pancrease
Western Blotting (WB) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (N-Term) antibody (ABIN2792537) Host: Mouse Target Name: SMAD3 Sample Tissue: Mouse Testis Antibody Dilution: ...
Immunohistochemistry (IHC) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (N-Term) antibody (ABIN2792537) Rabbit Anti-SMAD3 Antibody Formalin Fixed Paraffin Embedded Tissue: Human Urinary B...