anti-Dog (Canine) Endoplasmic Reticulum Protein 44 antibody for Immunohistochemistry

Recommended Endoplasmic Reticulum Protein 44 Antibody (supplied by: Log in to see )

Antigen
Endoplasmic Reticulum Protein 44 (ERP44) Antibodies
  • PDIA10
  • TXNDC4
  • 1110001E24Rik
  • AI849526
  • AL033348
  • Txndc4
  • BWK4
  • txndc4
  • zgc:77883
  • endoplasmic reticulum protein 44
  • ERP44
  • Erp44
  • erp44
Epitope
Internal Region
19
11
3
3
2
2
1
1
1
1
Reactivity
Bat, Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis
74
22
13
6
4
4
3
3
3
3
3
3
2
2
2
Host
Rabbit
50
17
9
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
52
38
27
23
9
6
3
2
Options
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Available images

Catalog No. ABIN1048579
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.973619 ABIN790276 IHC IHC (p) Rabbit C-Term Log in to see Polyclonal 0
1 ABIN2467583 IHC Rabbit C-Term Log in to see Polyclonal 0

General

Antigen Endoplasmic Reticulum Protein 44 (ERP44) Antibodies
Epitope Internal Region
(19), (11), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity Bat, Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis
(74), (22), (13), (6), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Host Rabbit
(50), (17), (9)
Clonality
Polyclonal
Conjugate Un-conjugated
(1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(52), (38), (27), (23), (9), (6), (3), (2)
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Product details

Target Details Application Details Handling Images
Brand IHC-plus™
Specificity Human ERP44 / TXNDC4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus (100%) Opossum, Stickleback, Medaka, Pufferfish, Zebrafish (95%).
Purification Immunoaffinity purified
Immunogen Synthetic 20 amino acid peptide from internal region of human ERP44 / TXNDC4. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus (100%), Opossum, Stickleback, Medaka, Pufferfish, Zebrafish (95%).

Type of Immunogen: Synthetic peptide

Target Details

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Antigen
Alternative Name ERP44 (ERP44 Antibody Abstract)
Background Name/Gene ID: ERP44

Synonyms: ERP44, ER protein 44, PDIA10, TXNDC4, KIAA0573
Gene ID 23071
Pathways Cell RedoxHomeostasis

Application Details

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Application Notes Approved: IHC, IHC-P (15 - 20 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

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Supplier Images
Immunohistochemistry (IHC) image for anti-Endoplasmic Reticulum Protein 44 (ERP44) (Internal Region) antibody (ABIN1048579) Anti-ERP44 / TXNDC4 antibody ABIN1048579 IHC staining of human placenta. Immunohisto...
Immunohistochemistry (IHC) image for anti-Endoplasmic Reticulum Protein 44 (ERP44) (Internal Region) antibody (ABIN1048579) Anti-ERP44 / TXNDC4 antibody ABIN1048579 IHC staining of human stomach. Immunohistoc...
Immunohistochemistry (IHC) image for anti-Endoplasmic Reticulum Protein 44 (ERP44) (Internal Region) antibody (ABIN1048579) Anti-ERP44 / TXNDC4 antibody IHC of human placenta. Immunohistochemistry of formalin-...