anti-Mouse (Murine) CD180 antibody for ELISA (Capture)

Recommended CD180 Antibody (supplied by: Log in to see )

Antigen
CD180 Molecule (CD180) Antibodies
  • Ly78
  • CD180
  • LY64
  • RP105
  • F630107B15
  • CD180 molecule
  • CD180 antigen
  • Cd180
  • CD180
Reactivity
Mouse (Murine)
92
32
3
2
Host
Rat
52
42
17
Clonality
Monoclonal
Conjugate
This CD180 antibody is conjugated to Biotin
12
11
10
4
Application
ELISA (Capture), Immunohistochemistry (IHC), Western Blotting (WB)
65
42
35
34
25
19
18
12
4
3
2
1
Options
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Catalog No. ABIN2660966
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General

Antigen CD180 Molecule (CD180) Antibodies
Reactivity Mouse (Murine)
(92), (32), (3), (2)
Host Rat
(52), (42), (17)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD180 antibody is conjugated to Biotin
(12), (11), (10), (4)
Application ELISA (Capture), Immunohistochemistry (IHC), Western Blotting (WB)
(65), (42), (35), (34), (25), (19), (18), (12), (4), (3), (2), (1)
Pubmed 5 references available
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Product Details anti-CD180 Antibody

Target Details CD180 Application Details Handling References for anti-CD180 antibody (ABIN2660966) Images
Purification The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Clone RP-14
Isotype IgG2a kappa
Plasmids, Primers & others

Target Details CD180

Product Details anti-CD180 Antibody Application Details Handling References for anti-CD180 antibody (ABIN2660966) Images back to top
Antigen
Alternative Name CD180 (CD180 Antibody Abstract)
Background CD180 is a 105 kD type I membrane protein, also known as RP105 or Ly64. It is a member of the toll-like receptor family consisting of an extracellular leucine-rich repeat (LRR) and a short cytoplasmic tail. It is expressed on most B cells, monocytes/macrophages, and dendritic cells. CD180 associates with MD-1 to form the RP105/MD-1 surface receptor. CD180/MD-1, by working in concert with TLR4, controls B cell recognition and signalling of bacterial LPS. The ligation of RP105 by RP/14 leads to a strong B cell proliferative response, as well as protection from apoptosis induced by radiation or dexamethasone. Interestingly, in some systems, ligation of RP105 leads to Fas-independent apoptosis.
Pathways Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades

Application Details

Product Details anti-CD180 Antibody Target Details CD180 Handling References for anti-CD180 antibody (ABIN2660966) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD180 Antibody Target Details CD180 Application Details References for anti-CD180 antibody (ABIN2660966) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD180 antibody (ABIN2660966)

Product Details anti-CD180 Antibody Target Details CD180 Application Details Handling Images back to top
Background publications

Chan, Mecklenbräuker, Su, Texido, Leitges, Carsetti, Lowell, Rajewsky, Miyake, Tarakhovsky: "The molecular mechanism of B cell activation by toll-like receptor protein RP-105." in: The Journal of experimental medicine, Vol. 188, Issue 1, pp. 93-101, 1998

Miyake, Shimazu, Kondo, Niki, Akashi, Ogata, Yamashita, Miura, Kimoto: "Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1348-53, 1998

Yamashita, Miyake, Miura, Kaneko, Yagita, Suda, Nagata, Nomura, Sakaguchi, Kimoto: "Activation mediated by RP105 but not CD40 makes normal B cells susceptible to anti-IgM-induced apoptosis: a role for Fc receptor coligation." in: The Journal of experimental medicine, Vol. 184, Issue 1, pp. 113-20, 1996

Miyake, Yamashita, Ogata, Sudo, Kimoto: "RP105, a novel B cell surface molecule implicated in B cell activation, is a member of the leucine-rich repeat protein family." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 7, pp. 3333-40, 1995

Miyake, Yamashita, Hitoshi, Takatsu, Kimoto: "Murine B cell proliferation and protection from apoptosis with an antibody against a 105-kD molecule: unresponsiveness of X-linked immunodeficient B cells." in: The Journal of experimental medicine, Vol. 180, Issue 4, pp. 1217-24, 1994

Images

Product Details anti-CD180 Antibody Target Details CD180 Application Details Handling References for anti-CD180 antibody (ABIN2660966) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD180 Molecule (CD180) antibody (Biotin) (ABIN2660966) anti-CD180 Molecule (CD180) antibody (Biotin)