anti-Mouse (Murine) CD180 antibody for Immunocytochemistry

Recommended CD180 Antibody (supplied by: Log in to see )

Antigen
CD180 Molecule (CD180) Antibodies
  • Ly78
  • CD180
  • LY64
  • RP105
  • F630107B15
  • CD180 molecule
  • CD180 antigen
  • Cd180
  • CD180
Reactivity
Mouse (Murine)
115
36
3
2
Host
Rat
72
45
20
Clonality
Monoclonal
Conjugate
This CD180 antibody is un-conjugated
13
13
11
5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
88
44
38
35
27
26
20
12
5
4
2
1
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Catalog No. ABIN2665573
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
-2.0244703 ABIN4350988 ELISA ICC IF IHC IHC (p) WB Rabbit Center Log in to see Polyclonal 0

General

Antigen CD180 Molecule (CD180) Antibodies
Reactivity Mouse (Murine)
(115), (36), (3), (2)
Host Rat
(72), (45), (20)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD180 antibody is un-conjugated
(13), (13), (11), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
(88), (44), (38), (35), (27), (26), (20), (12), (5), (4), (2), (1)
Pubmed 5 references available
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Product Details anti-CD180 Antibody

Target Details CD180 Application Details Handling References for anti-CD180 antibody (ABIN2665573) Images
Brand LEAF™
Purification The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level

Endotoxin level is <0.1 EU/μg of the protein (<0.01 ng/μg of the protein) as determined by the LAL test.

Clone RP-14
Isotype IgG2a kappa
Plasmids, Primers & others

Target Details CD180

Product Details anti-CD180 Antibody Application Details Handling References for anti-CD180 antibody (ABIN2665573) Images back to top
Antigen
Alternative Name CD180 (CD180 Antibody Abstract)
Background CD180 is a 105 kD type I membrane protein, also known as RP105 or Ly64. It is a member of the toll-like receptor family consisting of an extracellular leucine-rich repeat (LRR) and a short cytoplasmic tail. It is expressed on most B cells, monocytes/macrophages, and dendritic cells. CD180 associates with MD-1 to form the RP105/MD-1 surface receptor. CD180/MD-1, by working in concert with TLR4, controls B cell recognition and signalling of bacterial LPS. The ligation of RP105 by RP/14 leads to a strong B cell proliferative response, as well as protection from apoptosis induced by radiation or dexamethasone. Interestingly, in some systems, ligation of RP105 leads to Fas-independent apoptosis.
Pathways Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades

Application Details

Product Details anti-CD180 Antibody Target Details CD180 Handling References for anti-CD180 antibody (ABIN2665573) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD180 Antibody Target Details CD180 Application Details References for anti-CD180 antibody (ABIN2665573) Images back to top
Concentration 1.0 mg/mL
Preservative Azide free
Handling Advice This LEAF™ solution contains no preservative, handle under aseptic conditions
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD180 antibody (ABIN2665573)

Product Details anti-CD180 Antibody Target Details CD180 Application Details Handling Images back to top
Background publications

Chan, Mecklenbräuker, Su, Texido, Leitges, Carsetti, Lowell, Rajewsky, Miyake, Tarakhovsky: "The molecular mechanism of B cell activation by toll-like receptor protein RP-105." in: The Journal of experimental medicine, Vol. 188, Issue 1, pp. 93-101, 1998

Miyake, Shimazu, Kondo, Niki, Akashi, Ogata, Yamashita, Miura, Kimoto: "Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1348-53, 1998

Yamashita, Miyake, Miura, Kaneko, Yagita, Suda, Nagata, Nomura, Sakaguchi, Kimoto: "Activation mediated by RP105 but not CD40 makes normal B cells susceptible to anti-IgM-induced apoptosis: a role for Fc receptor coligation." in: The Journal of experimental medicine, Vol. 184, Issue 1, pp. 113-20, 1996

Miyake, Yamashita, Ogata, Sudo, Kimoto: "RP105, a novel B cell surface molecule implicated in B cell activation, is a member of the leucine-rich repeat protein family." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 7, pp. 3333-40, 1995

Miyake, Yamashita, Hitoshi, Takatsu, Kimoto: "Murine B cell proliferation and protection from apoptosis with an antibody against a 105-kD molecule: unresponsiveness of X-linked immunodeficient B cells." in: The Journal of experimental medicine, Vol. 180, Issue 4, pp. 1217-24, 1994

Images

Product Details anti-CD180 Antibody Target Details CD180 Application Details Handling References for anti-CD180 antibody (ABIN2665573) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD180 Molecule (CD180) antibody (ABIN2665573) anti-CD180 Molecule (CD180) antibody