anti-Human BCL6 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended BCL6 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 6 (BCL6) Antibodies
  • bcl5
  • laz3
  • bcl6a
  • znf51
  • zbtb27
  • MGC132260
  • Bcl5
  • BCL5
  • BCL6A
  • LAZ3
  • ZBTB27
  • ZNF51
  • B-cell CLL/lymphoma 6 L homeolog
  • B-cell CLL/lymphoma 6
  • B cell leukemia/lymphoma 6
  • bcl6.L
  • bcl6
  • BCL6
  • Bcl6
Epitope
AA 520-560
111
49
14
10
10
9
8
8
6
6
6
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
327
123
61
9
6
3
2
1
1
1
1
Host
Rabbit
193
129
5
1
Clonality
Polyclonal
Conjugate
This BCL6 antibody is un-conjugated
13
12
12
10
9
7
5
4
4
4
4
4
4
4
4
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
180
141
141
100
85
50
25
22
21
12
2
2
2
1
1
1
Options
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Catalog No. ABIN749843
$ 318.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN749847 IF (p) Cy5 Rabbit IgG AA 520-560 Log in to see Polyclonal 0
1 ABIN749848 IF (p) Cy5.5 Rabbit IgG AA 520-560 Log in to see Polyclonal 0
1 ABIN749849 IF (p) Cy7 Rabbit IgG AA 520-560 Log in to see Polyclonal 0
1 ABIN749846 IF (p) Cy3 Rabbit IgG AA 520-560 Log in to see Polyclonal 0
1 ABIN749850 IF (p) FITC Rabbit IgG AA 520-560 Log in to see Polyclonal 0
1 ABIN884680 IF (p) Alexa Fluor 488 Rabbit IgG AA 520-560 Log in to see Polyclonal 0
1 ABIN884681 IF (p) Alexa Fluor 555 Rabbit IgG AA 520-560 Log in to see Polyclonal 0
1 ABIN884682 IF (p) Alexa Fluor 647 Rabbit IgG AA 520-560 Log in to see Polyclonal 0
1 ABIN884684 IF (p) Alexa Fluor 350 Rabbit IgG AA 520-560 Log in to see Polyclonal 0
1 ABIN2810234 IF (p) Alexa Fluor 594 Rabbit IgG AA 520-560 Log in to see Polyclonal 0
1 ABIN4997291 IF (p) Alexa Fluor 750 Rabbit IgG AA 520-560 Log in to see Polyclonal 0
1 ABIN4997290 IF (p) Alexa Fluor 680 Rabbit IgG AA 520-560 Log in to see Polyclonal 0

General

Antigen B-Cell CLL/lymphoma 6 (BCL6) Antibodies
Epitope AA 520-560
(111), (49), (14), (10), (10), (9), (8), (8), (6), (6), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(327), (123), (61), (9), (6), (3), (2), (1), (1), (1), (1)
Host Rabbit
(193), (129), (5), (1)
Clonality
Polyclonal
Conjugate This BCL6 antibody is un-conjugated
(13), (12), (12), (10), (9), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(180), (141), (141), (100), (85), (50), (25), (22), (21), (12), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BCL6 Antibody

Target Details BCL6 Application Details Handling References for anti-BCL6 antibody (ABIN749843) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human Bcl6
Isotype IgG
Plasmids, Primers & others

Target Details BCL6

Product Details anti-BCL6 Antibody Application Details Handling References for anti-BCL6 antibody (ABIN749843) Images back to top
Antigen
Alternative Name Bcl6 (BCL6 Antibody Abstract)
Background

Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4(+) T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.

Subcellular location: Nucleus

Synonyms: BCL5, LAZ3, BCL6A, ZNF51, ZBTB27, B-cell lymphoma 6 protein, BCL-6, B-cell lymphoma 5 protein, BCL-5, Protein LAZ-3, Zinc finger and BTB domain-containing protein 27, Zinc finger protein 51, BCL6

Gene ID 604
UniProt P41182
Pathways Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus

Application Details

Product Details anti-BCL6 Antibody Target Details BCL6 Handling References for anti-BCL6 antibody (ABIN749843) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-BCL6 Antibody Target Details BCL6 Application Details References for anti-BCL6 antibody (ABIN749843) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-BCL6 antibody (ABIN749843)

Product Details anti-BCL6 Antibody Target Details BCL6 Application Details Handling Images back to top
Product cited in:

Szabó, Gáspár, Dajnoki, Papp, Fábos, Szegedi, Zeher: "Expansion of circulating follicular T helper cells associates with disease severity in childhood atopic dermatitis." in: Immunology letters, Vol. 189, pp. 101-108, 2017 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human). Further details: Sample: skin

Images

Product Details anti-BCL6 Antibody Target Details BCL6 Application Details Handling References for anti-BCL6 antibody (ABIN749843) back to top
Supplier Images
SDS-PAGE (SDS) image for anti-B-Cell CLL/lymphoma 6 (BCL6) (AA 520-560) antibody (ABIN749843) Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti...
SDS-PAGE (SDS) image for anti-B-Cell CLL/lymphoma 6 (BCL6) (AA 520-560) antibody (ABIN749843) Lane 1: mouse heart lysates Lane 2: mouse brain lysates probed with Anti BCL-6/5 Anti...
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 6 (BCL6) (AA 520-560) antibody (ABIN749843) Lane 1:mouse brain lysates probed with Rabbit Anti-Bcl6 Polyclonal Antibody, Unconjug...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-B-Cell CLL/lymphoma 6 (BCL6) (AA 520-560) antibody (ABIN749843) Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti...