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|Antigen||Recombination Activating Gene 2 (RAG2) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This RAG2 antibody is conjugated to Alexa Fluor 350 Alternatives|
Immunofluorescence (Paraffin-embedded Sections) (IF (p))
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Product Details anti-RAG2 AntibodyTarget Details RAG2 Application Details Handling Images
|Purification||Purified by Protein A.|
|Immunogen||KLH conjugated synthetic peptide derived from human RAG2|
Target Details RAG2Product Details anti-RAG2 Antibody Application Details Handling Images back to top
|Alternative Name||Rag2 (RAG2 Antibody Abstract)|
Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination.
Synonyms: RAG 2, RAG-2, RAG2, RAG2_HUMAN, Recombination activating gene 2, VDJ recombination activating protein 2, VDJ recombination-activating protein 2.
|Pathways||Chromatin Binding, Production of Molecular Mediator of Immune Response|
Application DetailsProduct Details anti-RAG2 Antibody Target Details RAG2 Handling Images back to top
|Application Notes||IF(IHC-P) 1:50-200|
|Restrictions||For Research Use only|
HandlingProduct Details anti-RAG2 Antibody Target Details RAG2 Application Details Images back to top
|Buffer||Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.|
|Storage Comment||Store at 4°C|
|Expiry Date||12 months|