anti-Human C1S antibody for Flow Cytometry

Recommended C1S Antibody (supplied by: Log in to see )

Antigen
Complement Component 1, S Subcomponent (C1S) Antibodies
  • AA959438
  • AI255193
  • AI327365
  • C1sa
  • r-gsp
  • C1S
  • complement C1s
  • complement component 1, s subcomponent 1
  • complement component 1, s subcomponent
  • complement component 1, s subcomponent L homeolog
  • complement C1s subcomponent
  • C1S
  • C1s1
  • C1s
  • c1s
  • c1s.L
  • LOC100558615
Reactivity
Human
115
25
24
4
1
Host
Mouse
64
48
4
Clonality
Monoclonal
Conjugate
This C1S antibody is un-conjugated
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Inhibition Assay (InhA), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
69
46
36
15
14
13
12
6
5
3
3
3
2
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN2191986
$ 647.55
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
15.792408 ABIN2716898 FACS IHC WB Mouse IgG2a Log in to see 2D11 0
15.792408 ABIN5649735 FACS IHC (p) WB Mouse IgG2a Log in to see 2D11 0
12.811565 ABIN2135115 FACS IHC WB Mouse IgG2a Log in to see 2D11 0
12.811565 ABIN2135114 FACS IF IHC WB Mouse IgG2a Log in to see 5F2 0
11.034214 ABIN4299882 FACS ICC IF IHC IHC (p) WB Mouse IgG2a Log in to see 4E3 0
10 ABIN2716897 FACS IF IHC WB Mouse IgG2a Log in to see 5F2 0
10 ABIN2716896 FACS IF IHC WB Mouse IgG2a Log in to see 4E3 0
4 ABIN2716893 FACS WB Mouse IgG1 Log in to see 4D9 0
4 ABIN2716895 FACS WB Mouse IgG1 Log in to see 2F5 0
1 ABIN2962082 FACS IHC (p) WB Mouse IgG2a full length Log in to see 2D11 0
-12.758193 ABIN6246515 FACS ICC IF IHC WB Mouse IgG2a Log in to see OTI4E3 0
-12.758193 ABIN4299880 BP FACS IA IHC IHC (fro) IHC (p) Neut WB Mouse IgG2a Log in to see M241 0

General

Antigen Complement Component 1, S Subcomponent (C1S) Antibodies
Reactivity Human
(115), (25), (24), (4), (1)
Host Mouse
(64), (48), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C1S antibody is un-conjugated
(3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Inhibition Assay (InhA), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(69), (46), (36), (15), (14), (13), (12), (6), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-C1S Antibody

Target Details C1S Application Details Handling References for anti-C1S antibody (ABIN2191986) Images
Sterility 0.2 μm filtered
Clone M81
Plasmids, Primers & others

Target Details C1S

Product Details anti-C1S Antibody Application Details Handling References for anti-C1S antibody (ABIN2191986) Images back to top
Antigen
Alternative Name c1s (C1S Antibody Abstract)
Background Monoclonal antibody M81 reacts with an epitope on human C protein activated C1s, a subcomponent of the first component of C (C1). Activated C1s is a glycosylated single-polypeptide zymogen, MW 85 kD. Activation of the proenzyme C1s occurs through cleavage by the active form of C1r. The activated protease, activated C1s, consists of a disulfide-linked H chain and a L chain. Activated C1s is a serine protease and its catalytic site is located in the L chain. Activation of the classical C pathway is triggered by activated C1s which cleaves C4 and C2 to form the C3 convertase, C4bC2a. The epitope recognised by the antibody M81 is domain IV and/or V of the gamma-domain of activated C1s. Monoclonal antibody M81 blocks C4 activation and C4 binding to activated C1s. The antibody reacts around the binding site of C1s and reacts with both active and inactive C1s.
Pathways Complement System

Application Details

Product Details anti-C1S Antibody Target Details C1S Handling References for anti-C1S antibody (ABIN2191986) Images back to top
Application Notes For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of C4 activation by C1s dilutions have to be made according to the amounts C1s to be inactivated.
Restrictions For Research Use only

Handling

Product Details anti-C1S Antibody Target Details C1S Application Details References for anti-C1S antibody (ABIN2191986) Images back to top
Buffer PBS containing 0.1 % bovine serum albumin.
Storage 4 °C
Storage Comment Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Expiry Date 12 months

References for anti-C1S antibody (ABIN2191986)

Product Details anti-C1S Antibody Target Details C1S Application Details Handling Images back to top
Product cited in:

Matsumoto, Nagaki, Kitamura, Kuramitsu, Nagasawa, Seya: "Probing the C4-binding site on C1s with monoclonal antibodies. Evidence for a C4/C4b-binding site on the gamma-domain." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2743-50, 1989