anti-Human C3 antibody for Flow Cytometry

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • AHUS5
  • ARMD9
  • ASP
  • C3a
  • C3b
  • CPAMD1
  • AI255234
  • HSE-MSF
  • Plp
  • C3
  • C3-1
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
  • C3d
  • asp
  • cpamd1
  • c3
  • complement C3
  • complement component 3
  • complement component c3a, duplicate 1
  • complement component 3 L homeolog
  • complement component C3
  • complement C3-like
  • C3
  • c3a.1
  • c3.L
  • LOC443475
  • c3
  • c3-1
  • LOC100060539
Reactivity
Human
273
79
66
16
9
7
6
5
4
3
1
1
Host
Mouse
149
118
94
27
14
7
Clonality
Monoclonal
Conjugate
This C3 antibody is un-conjugated
36
35
21
5
5
5
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunoassay (IA), Flow Cytometry (FACS), Western Blotting (WB)
211
189
101
69
69
62
55
51
45
38
31
26
17
9
6
5
4
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN2191942
$ 595.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10.918978 ABIN5680961 ELISA FACS ICC IF IHC WB Mouse IgG1 AA 1521-1649 Log in to see 4C6A8 0
5.5 ABIN2477804 IHC (fro) FACS ELISA Mouse IgG1 Log in to see 053A-514-3-1-4 2
5.5 ABIN2477803 IHC (fro) FACS ELISA WB Mouse IgG1 Log in to see 053A-1149-3-1-4 (also known as 1003) 1
4 ABIN308050 FACS ELISA FITC Mouse IgG1 Log in to see 6C9 0
4 ABIN5565072 FACS ICC IF IHC PE Rabbit Log in to see Polyclonal 0
1 ABIN317012 EIA FACS IHC (fro) WB Mouse IgG2b Log in to see 013III-1-16 3
1 ABIN181129 EIA FACS IHC (fro) Mouse IgG1 Log in to see 053A-514-3-1-4 2
1 ABIN181106 EIA FACS IHC (fro) WB Mouse IgG1 Log in to see 10-02A 1
1 ABIN181107 EIA FACS IHC (fro) WB Mouse IgG1 Log in to see 053A-1149-3-1-4 (1003) 2
1 ABIN308054 FACS ELISA PE Mouse IgG1 Log in to see 10C7 0
1 ABIN308049 FACS ELISA Biotin Mouse IgG1 Log in to see 6C9 0
1 ABIN308052 FACS ELISA Biotin Mouse IgG1 Log in to see 10C7 0
1 ABIN308051 FACS ELISA PE Mouse IgG1 Log in to see 6C9 0
1 ABIN308053 FACS FITC Mouse IgG1 Log in to see 10C7 0
1 ABIN601081 ELISA FACS IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 0
1 ABIN601082 ELISA FACS IHC IHC (fro) IHC (p) Mouse IgG1 Log in to see 0
1 ABIN212862 FACS ELISA Mouse IgG1 Log in to see 6C9 0
1 ABIN212863 FACS ELISA Mouse IgG1 Log in to see 10C7 0
1 ABIN206674 ELISA FACS IHC IHC (fro) Mouse IgG1 Log in to see 0
1 ABIN951635 EIA FACS WB Chicken Ig Fraction Log in to see Polyclonal 1

General

Antigen Complement Component 3 (C3) Antibodies
Reactivity Human
(273), (79), (66), (16), (9), (7), (6), (5), (4), (3), (1), (1)
Host Mouse
(149), (118), (94), (27), (14), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C3 antibody is un-conjugated
(36), (35), (21), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunoassay (IA), Flow Cytometry (FACS), Western Blotting (WB)
(211), (189), (101), (69), (69), (62), (55), (51), (45), (38), (31), (26), (17), (9), (6), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling References for anti-C3 antibody (ABIN2191942) Images
Cross-Reactivity (Details) Cross reactivity: Human C3a : No
Sterility 0.2 μm filtered
Clone 755
Isotype IgG1
Plasmids, Primers & others

Target Details C3

Product Details anti-C3 Antibody Application Details Handling References for anti-C3 antibody (ABIN2191942) Images back to top
Antigen
Alternative Name c3/c3b (C3 Antibody Abstract)
Background The monoclonal antibody 755 recognizes an epitope located in the C-terminal 360 amino acids on the alpha chain of C3, thereby recognizing C3b and full C3. The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3 mg/mL. An inherited deficiency of C3 predisposes a person to frequent bacterial infections. C3 fragments are deposited in tissues at sites of antibody- mediated immunopathology. In ulcerative colitis and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported. After activation of the complement system, certain enzymes become active, resulting in the cleavage of C3 into C3b and the anaphylatoxin C3a. C3b becomes attached to immune complexes and is further cleaved into iC3b, C3c, C3dg and C3f. Within the alternative pathway of complement, C3b plays a critical role in the amplification loop initiated by spontaneous hydrolysis of C3. Aliases Complement component 3 Immunogen Native C3
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling References for anti-C3 antibody (ABIN2191942) Images back to top
Application Notes For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive Human serum control Negative C3 deficient serum control
Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details References for anti-C3 antibody (ABIN2191942) Images back to top
Buffer PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-C3 antibody (ABIN2191942)

Product Details anti-C3 Antibody Target Details C3 Application Details Handling Images back to top
Product cited in:

Hawlisch, Meyer zu Vilsendorf, Bautsch, Klos, Köhl: "Guinea pig C3 specific rabbit single chain Fv antibodies from bone marrow, spleen and blood derived phage libraries." in: Journal of immunological methods, Vol. 236, Issue 1-2, pp. 117-31, 2000

Klos, Ihrig, Messner, Grabbe, Bitter-Suermann: "Detection of native human complement components C3 and C5 and their primary activation peptides C3a and C5a (anaphylatoxic peptides) by ELISAs with monoclonal antibodies." in: Journal of immunological methods, Vol. 111, Issue 2, pp. 241-52, 1988