anti-Human C3 antibody for Western Blotting

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • AHUS5
  • ARMD9
  • ASP
  • C3a
  • C3b
  • CPAMD1
  • AI255234
  • HSE-MSF
  • Plp
  • C3
  • C3-1
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
  • C3d
  • asp
  • cpamd1
  • c3
  • complement C3
  • complement component 3
  • complement component c3a, duplicate 1
  • complement component 3 L homeolog
  • complement component C3
  • complement C3-like
  • C3
  • c3a.1
  • c3.L
  • LOC443475
  • c3
  • c3-1
  • LOC100060539
Epitope
C-Term
15
14
6
6
5
4
4
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
273
78
66
16
9
7
6
5
4
3
1
1
Host
Rabbit
150
117
94
27
14
7
Clonality
Polyclonal
Conjugate
This C3 antibody is un-conjugated
36
35
21
5
5
5
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunosubtraction (ISub), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
211
189
101
69
68
61
56
50
45
38
30
26
17
10
6
5
4
2
2
2
1
1
1
1
1
1
1
Options
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Available images

Immunoprecipitation (IP) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN2855007)
Immunofluorescence (IF) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN2855007)
Immunohistochemistry (IHC) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN2855007)
Western Blotting (WB) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN2855007)
Catalog No. ABIN2855007
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.250505 ABIN2838577 WB Mouse IgG2a kappa AA 1534-1645 Log in to see 5F9 4
19.750505 ABIN1173819 ICC IHC WB Rabbit IgG AA 1000-1326 Log in to see Polyclonal 0
16.88187 ABIN2191942 IA FACS WB Mouse IgG1 Log in to see 755 2
13.750505 ABIN2561506 IHC WB Rabbit IgG Log in to see Polyclonal 0
13.750505 ABIN560117 IHC (p) ELISA WB Mouse IgG2a kappa AA 1534-1644 Log in to see 5F9 0
12.38187 ABIN871084 WB Rabbit IgG Chain beta Log in to see Polyclonal 0
12.38187 ABIN5617665 ELISA WB Goat Log in to see Polyclonal 0
12.38187 ABIN5621831 WB Rabbit IgG Log in to see Polyclonal 0
12.38187 ABIN2138033 ELISA WB Mouse IgG2b kappa Log in to see 28A1 0
12.38187 ABIN2138032 ELISA WB Mouse IgG2b kappa Log in to see 10A1 0
12.38187 ABIN2688507 ELISA WB Mouse IgG1 kappa Log in to see M81566 0
12.38187 ABIN2688496 ELISA WB Mouse IgG1 kappa Log in to see M81574 0
12.38187 ABIN2688499 ELISA WB Mouse IgG1 kappa Log in to see M81482 0
10.750505 ABIN3048517 IHC WB Rabbit IgG Log in to see Polyclonal 0
10.750505 ABIN2896723 IF/ICC IHC IP WB Rabbit AA 1000-1326 Log in to see Polyclonal 0
7.750506 ABIN513865 ELISA WB Mouse IgG2a kappa AA 1534-1644 Log in to see 5F9 0
7.750506 ABIN5544817 ELISA WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN337267 ELISA IHC IHC (p) WB Mouse IgG1, kappa Log in to see 2D8 0
7 ABIN574215 ELISA IHC IHC (p) WB Mouse IgG2a, kappa AA 1534-1645 Log in to see 5F9 0
5.811133 ABIN442056 ICC IF IHC (p) IP SimWes WB Rabbit IgG C-Term Log in to see Polyclonal 0

General

Antigen Complement Component 3 (C3) Antibodies
Epitope C-Term
(15), (14), (6), (6), (5), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(273), (78), (66), (16), (9), (7), (6), (5), (4), (3), (1), (1)
Host Rabbit
(150), (117), (94), (27), (14), (7)
Clonality
Polyclonal
Conjugate This C3 antibody is un-conjugated
(36), (35), (21), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunosubtraction (ISub), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(211), (189), (101), (69), (68), (61), (56), (50), (45), (38), (30), (26), (17), (10), (6), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling Images
Characteristics Rabbit Polyclonal antibody to C3 (complement component 3)
C3 antibody [C3], C-term
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human C3. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details C3

Product Details anti-C3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name C3 (C3 Antibody Abstract)
Background Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection.

Cellular Localization: Secreted
Molecular Weight 187 kDa
Gene ID 718
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Immunosubtraction Assay-dependent dilution Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000* ImmunosubtractionAssay-dependent dilution
Comment

Positive Control: Huh7 , HepG2
Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details Images back to top
Format Liquid
Concentration 0.62 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-C3 Antibody Target Details C3 Application Details Handling back to top
Supplier Images
Immunoprecipitation (IP) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN2855007) IP Image Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 μg ...
Immunofluorescence (IF) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN2855007) ICC/IF Image C3 antibody [C3], C-term detects C3 protein at cytoplasm by immunofluore...
Immunohistochemistry (IHC) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN2855007) IHC-P Image C3 antibody [C3], C-term detects C3 protein at cytoplasm in mouse brain b...
Western Blotting (WB) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN2855007) WB Image C3 antibody detects C3 protein by western blot analysis. Various whole cell ...