anti-Human C3 antibody for Western Blotting

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • AHUS5
  • ARMD9
  • ASP
  • C3a
  • C3b
  • CPAMD1
  • AI255234
  • HSE-MSF
  • Plp
  • C3
  • C3-1
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
  • C3d
  • asp
  • cpamd1
  • c3
  • complement C3
  • complement component 3
  • complement component c3a, duplicate 1
  • complement component 3 L homeolog
  • complement component C3
  • complement C3-like
  • C3
  • c3a.1
  • c3.L
  • LOC443475
  • c3
  • c3-1
  • LOC100060539
Epitope
C-Term
15
14
6
6
5
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
284
78
66
16
15
9
7
6
5
4
3
1
Host
Rabbit
160
131
95
27
14
7
Clonality
Polyclonal
Conjugate
This C3 antibody is un-conjugated
38
37
23
7
7
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunosubtraction (ISub), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
222
198
101
71
69
59
56
50
45
39
38
30
17
10
5
5
5
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN2855007
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.302324 ABIN2838577 WB Mouse IgG2a kappa AA 1534-1645 Log in to see 5F9 4
19.802324 ABIN1173819 ICC IHC WB Rabbit IgG AA 1000-1326 Log in to see Polyclonal 0
17.260653 ABIN2191942 IA FACS WB Mouse IgG1 Log in to see 755 2
13.802324 ABIN2561506 IHC WB Rabbit IgG Log in to see Polyclonal 0
13.802324 ABIN560117 IHC (p) ELISA WB Mouse IgG2a kappa AA 1534-1644, partial Log in to see 5F9 0
12.760653 ABIN871084 WB Rabbit IgG Chain beta Log in to see Polyclonal 0
12.760653 ABIN5617665 ELISA WB Goat Log in to see Polyclonal 0
12.760653 ABIN5621831 WB Rabbit IgG Log in to see Polyclonal 0
12.760653 ABIN2688499 ELISA WB Mouse IgG1 kappa Log in to see M81482 0
12.760653 ABIN2688496 ELISA WB Mouse IgG1 kappa Log in to see M81574 0
12.760653 ABIN2138032 ELISA WB Mouse IgG2b kappa Log in to see 10A1 0
12.760653 ABIN2138033 ELISA WB Mouse IgG2b kappa Log in to see 28A1 0
12.760653 ABIN2688507 ELISA WB Mouse IgG1 kappa Log in to see M81566 0
10.802324 ABIN3048517 IHC WB Rabbit IgG Log in to see Polyclonal 0
10.802324 ABIN2896723 IF/ICC IHC IP WB Rabbit AA 1000-1326 Log in to see Polyclonal 0
7.8023243 ABIN513865 ELISA WB Mouse IgG2a kappa AA 1534-1644, partial Log in to see 5F9 0
7.8023243 ABIN5544817 ELISA WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN337267 ELISA IHC IHC (p) WB Mouse IgG1, kappa Log in to see 2D8 0
7 ABIN574215 ELISA IHC IHC (p) WB Mouse IgG2a, kappa AA 1534-1645 Log in to see 5F9 0
5.5 ABIN2477803 IHC (fro) FACS ELISA WB Mouse IgG1 Log in to see 053A-1149-3-1-4 (also known as 1003) 1

General

Antigen Complement Component 3 (C3) Antibodies
Epitope C-Term
(15), (14), (6), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(284), (78), (66), (16), (15), (9), (7), (6), (5), (4), (3), (1)
Host Rabbit
(160), (131), (95), (27), (14), (7)
Clonality
Polyclonal
Conjugate This C3 antibody is un-conjugated
(38), (37), (23), (7), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunosubtraction (ISub), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(222), (198), (101), (71), (69), (59), (56), (50), (45), (39), (38), (30), (17), (10), (5), (5), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling Images
Characteristics Rabbit Polyclonal antibody to C3 (complement component 3)
C3 antibody [C3], C-term
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human C3. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details C3

Product Details anti-C3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name C3 (C3 Antibody Abstract)
Background Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection.

Cellular Localization: Secreted
Molecular Weight 187 kDa
Gene ID 718
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Immunosubtraction Assay-dependent dilution Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000* ImmunosubtractionAssay-dependent dilution
Comment

Positive Control: Huh7 , HepG2

Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details Images back to top
Format Liquid
Concentration 0.62 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-C3 Antibody Target Details C3 Application Details Handling back to top
Supplier Images
Immunoprecipitation (IP) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN2855007) IP Image Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 μg ...
Immunofluorescence (IF) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN2855007) ICC/IF Image C3 antibody [C3], C-term detects C3 protein at cytoplasm by immunofluore...
Immunohistochemistry (IHC) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN2855007) IHC-P Image C3 antibody [C3], C-term detects C3 protein at cytoplasm in mouse brain b...
Western Blotting (WB) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN2855007) WB Image C3 antibody detects C3 protein by western blot analysis. Various whole cell ...