anti-Human C3 antibody for Western Blotting

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • AHUS5
  • ARMD9
  • ASP
  • C3a
  • C3b
  • CPAMD1
  • AI255234
  • HSE-MSF
  • Plp
  • C3
  • C3-1
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
  • C3d
  • asp
  • cpamd1
  • c3
  • complement C3
  • complement component 3
  • complement component c3a, duplicate 1
  • complement component 3 L homeolog
  • complement component C3
  • complement C3-like
  • C3
  • c3a.1
  • c3.L
  • LOC443475
  • c3
  • c3-1
  • LOC100060539
Epitope
C-Term
15
14
6
6
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
285
79
67
16
15
9
7
6
5
4
3
1
Host
Rabbit
160
132
95
27
14
7
Clonality
Polyclonal
Conjugate
This C3 antibody is un-conjugated
38
37
23
7
7
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
223
198
101
71
69
59
56
50
45
39
38
30
17
10
5
5
5
2
2
2
1
1
1
1
1
1
1
Supplier
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Available images

Immunoprecipitation (IP) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN442056)
Simple Western (SimWes) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN442056)
Immunofluorescence (IF) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN442056)
Western Blotting (WB) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN442056)
Western Blotting (WB) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN442056)
Western Blotting (WB) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN442056)
Catalog No. ABIN442056
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.08196 ABIN2838577 WB Mouse IgG2a kappa AA 1534-1645 Log in to see 5F9 2
3.08196 ABIN2855007 ICC IF ISub IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.08196 ABIN5680961 ELISA FACS ICC IF IHC WB Mouse IgG1 AA 1521-1649 Log in to see 4C6A8 0
3.08196 ABIN1173819 ICC IHC WB Rabbit IgG AA 1000-1326 Log in to see Polyclonal 0
3.08196 ABIN2561506 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.08196 ABIN560117 IHC (p) ELISA WB Mouse IgG2a kappa AA 1534-1644, partial Log in to see 5F9 0
3.08196 ABIN513865 ELISA WB Mouse IgG2a kappa AA 1534-1644, partial Log in to see 5F9 0
3.08196 ABIN2896695 ELISA WB Rabbit IgG AA 1321-1663 Log in to see Polyclonal 0
3.08196 ABIN3048517 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.08196 ABIN1173821 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 0
3.08196 ABIN2896723 IF/ICC IHC IP WB Rabbit AA 1000-1326 Log in to see Polyclonal 0
3.08196 ABIN5544817 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN337267 ELISA IHC IHC (p) WB Mouse IgG1, kappa Log in to see 2D8 0
1 ABIN574215 ELISA IHC IHC (p) WB Mouse IgG2a, kappa AA 1534-1645 Log in to see 5F9 0
1 ABIN317012 EIA FACS IHC (fro) WB Mouse IgG2b Log in to see 013III-1-16 3
1 ABIN269584 IA WB Mouse IgG1 Log in to see 474 4
1 ABIN181105 EIA WB Mouse IgG1 Log in to see 01-10-21 2
1 ABIN333117 ELISA WB Mouse IgG2b, kappa Log in to see 0
1 ABIN333118 ELISA WB Mouse IgG2b, kappa Log in to see 0
1 ABIN568570 DB EIA ID IHC (fro) IF IP WB Biotin Goat IgG Log in to see Polyclonal 0

General

Antigen Complement Component 3 (C3) Antibodies
Epitope C-Term
(15), (14), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(285), (79), (67), (16), (15), (9), (7), (6), (5), (4), (3), (1)
Host Rabbit
(160), (132), (95), (27), (14), (7)
Clonality
Polyclonal
Conjugate This C3 antibody is un-conjugated
(38), (37), (23), (7), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(223), (198), (101), (71), (69), (59), (56), (50), (45), (39), (38), (30), (17), (10), (5), (5), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling Images
Purification Immunogen affinity purified
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human C3. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details C3

Product Details anti-C3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background Gene Symbol: C3
Molecular Weight Theoretical MW: 187 kDa
Gene ID 718
UniProt P01024
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling Images back to top
Application Notes Western Blot 1:500-1:3000, Simple Western 1:50, Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunoprecipitation 1:100-1:500, Immunohistochemistry-Paraffin 1:100-1:1000Immunosubtraction. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details Images back to top
Format Liquid
Concentration 0.16 mg/mL
Buffer PBS ( pH 7.0) and 20 % Glycerol
Buffer contains: 0.025 % Proclin 300
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Images

Product Details anti-C3 Antibody Target Details C3 Application Details Handling back to top
Supplier Images
Immunoprecipitation (IP) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN442056) Immunoprecipitation: Complement C3 Antibody [NBP1-32080] - Immunoprecipitation of C3 ...
Simple Western (SimWes) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN442056) Simple Western: Complement C3 Antibody [NBP1-32080] - Simple Western lane view shows ...
Immunofluorescence (IF) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN442056) Immunocytochemistry/Immunofluorescence: Complement C3 Antibody [NBP1-32080] - HeLa ce...
Western Blotting (WB) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN442056) Western Blot: Complement C3 Antibody [NBP1-32080] - A: Non-transfected 293T lysates B...
Western Blotting (WB) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN442056) Western Blot: Complement C3 Antibody [NBP1-32080] - Lane A: Non-transfected 293T lysa...
Western Blotting (WB) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN442056) Western Blot: Complement C3 Antibody [NBP1-32080] - A:Hep G2.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN442056) Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-32080] - Paraffin-embedde...
Western Blotting (WB) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN442056) Western Blot: Complement C3 Antibody [NBP1-32080] - A. 293T whole cell lysate/extract...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN442056) Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-32080] - Paraffin-embedde...
Immunofluorescence (IF) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN442056) Immunocytochemistry/Immunofluorescence: Complement C3 Antibody [NBP1-32080] - Parafor...
Western Blotting (WB) image for anti-Complement Component 3 (C3) (C-Term) antibody (ABIN442056) Western Blot: Complement C3 Antibody [NBP1-32080] - Various whole cell extracts (30 u...