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General |
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Antigen | Complement Component 3 (C3) Antibodies |
Epitope | AA 1580-1592 Alternatives |
Reactivity | Human, Monkey Alternatives |
Host | Goat Alternatives |
Clonality | |
Conjugate | This C3 antibody is un-conjugated Alternatives |
Application |
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Alternatives
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Supplier | Log in to see |
Product Details anti-C3 AntibodyTarget Details C3 Application Details Handling Images |
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Brand | IHC-plus™ |
Specificity | Human Complement C3. This antibody is expected to recognize the C3 alpha chain, C3b alpha chain and the C3c alpha chain fragment 2. |
Purification | Immunoaffinity purified |
Immunogen |
Synthetic peptide QQRTFISPIKCRE from the C-terminus of human Complement C3 (NP_000055.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%). Type of Immunogen: Synthetic peptide |
Plasmids, Primers & others | |
Target Details C3Product Details anti-C3 Antibody Application Details Handling Images back to top |
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Antigen | |
Alternative Name | Complement C3 (C3 Antibody Abstract) |
Background |
Name/Gene ID: C3 Synonyms: C3, AHUS5, ARMD9, Complement component C3, CPAMD1, Complement C3, ASP, Complement component 3 |
Gene ID | 718 |
NCBI Accession | NP_000055 |
Pathways | Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling |
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Application Notes |
Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000) Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave an approx 125kD band in Human Liver lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kD according to the largest fragment C3 alpha chain (aa672-1663). Although this fragment is glycosylated, we have not yet found support in the literature for the observed size. The 125kD band was successfully blocked by incubation with the immunizing peptide. |
Comment |
Target Species of Antibody: Human |
Restrictions | For Research Use only |
HandlingProduct Details anti-C3 Antibody Target Details C3 Application Details Images back to top |
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Format | Liquid |
Concentration | Lot specific |
Buffer | Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Minimize freezing and thawing. |
Storage | -20 °C |
Storage Comment | Store at -20°C. Avoid freeze-thaw cycles. |
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Supplier Images |