anti-Pig (Porcine) C3 antibody for Western Blotting

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • AHUS5
  • ARMD9
  • ASP
  • C3a
  • C3b
  • CPAMD1
  • AI255234
  • HSE-MSF
  • Plp
  • C3
  • C3-1
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
  • C3d
  • asp
  • cpamd1
  • c3
  • complement C3
  • complement component 3
  • complement component c3a, duplicate 1
  • complement component 3 L homeolog
  • complement component C3
  • complement C3-like
  • C3
  • c3a.1
  • c3.L
  • LOC443475
  • c3
  • c3-1
  • LOC100060539
Epitope
AA 23-300
16
15
6
6
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Pig (Porcine), Wild boar (Sus scrofa)
291
80
71
16
15
9
7
6
4
4
3
Host
Rabbit
166
132
97
27
14
8
Clonality
Polyclonal
Conjugate
This C3 antibody is un-conjugated
38
37
23
7
7
6
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
227
206
100
76
69
61
56
51
45
39
38
30
17
11
5
5
5
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN1862261
$ 378.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0962598 ABIN2896726 IF/ICC IHC IP WB Rabbit AA 23-300 Log in to see Polyclonal 0
1 ABIN6021954 IF/ICC IHC IP WB Mouse Log in to see 0

General

Antigen Complement Component 3 (C3) Antibodies
Epitope AA 23-300
(16), (15), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Pig (Porcine), Wild boar (Sus scrofa)
(291), (80), (71), (16), (15), (9), (7), (6), (4), (4), (3)
Host Rabbit
(166), (132), (97), (27), (14), (8)
Clonality
Polyclonal
Conjugate This C3 antibody is un-conjugated
(38), (37), (23), (7), (7), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
(227), (206), (100), (76), (69), (61), (56), (51), (45), (39), (38), (30), (17), (11), (5), (5), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling References for anti-C3 antibody (ABIN1862261) Images
Specificity The antibody is a rabbit polyclonal antibody raised against C3. It has been selected for its ability to recognize C3 in immunohistochemical staining and western blotting.
Purification Antigen-specific affinity chromatography
Immunogen C3 (Asp23-Thr300)
Isotype IgG
Plasmids, Primers & others

Target Details C3

Product Details anti-C3 Antibody Application Details Handling References for anti-C3 antibody (ABIN1862261) Images back to top
Antigen
Alternative Name Complement Component 3 (C3 Antibody Abstract)
Background Alternative Names: ASP, CPAMD1, Complement Protein C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a anaphylatoxin, Acylation stimulating protein
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling References for anti-C3 antibody (ABIN1862261) Images back to top
Application Notes
  • Western blotting: 0.5-2 μg/mL
  • Immunocytochemistry in formalin fixed cells: 5-20 μg/mL
  • Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL
  • Immunohistochemistry in paraffin section: 5-20 μg/mL
  • Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL
  • Optimal working dilutions must be determined by end user.
Comment

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details References for anti-C3 antibody (ABIN1862261) Images back to top
Format Liquid
Concentration 200 μg/mL
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

References for anti-C3 antibody (ABIN1862261)

Product Details anti-C3 Antibody Target Details C3 Application Details Handling Images back to top
Product cited in:

Zhang, Wang, Wang, Jin, Zhao, Xiong, Zhang, Mou, Li, Yang, Dai: "Generation of complement protein C3 deficient pigs by CRISPR/Cas9-mediated gene targeting." in: Scientific reports, Vol. 7, Issue 1, pp. 5009, 2017

Images

Product Details anti-C3 Antibody Target Details C3 Application Details Handling References for anti-C3 antibody (ABIN1862261) back to top
Supplier Images
Western Blotting (WB) image for anti-Complement Component 3 (C3) (AA 23-300) antibody (ABIN1862261) anti-Complement Component 3 (C3) (AA 23-300) antibody
Western Blotting (WB) image for anti-Complement Component 3 (C3) (AA 23-300) antibody (ABIN1862261) Figure. Western Blot; Sample: Lane1: Porcine Serum ; Lane2: Porcine Liver Tissue; Lan...
 image for anti-Complement Component 3 (C3) (AA 23-300) antibody (ABIN1862261) DAB staining on IHC-P; Samples: Porcine Liver Tissue