anti-Rabbit C3 antibody for Western Blotting

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • AHUS5
  • ARMD9
  • ASP
  • C3a
  • C3b
  • CPAMD1
  • AI255234
  • HSE-MSF
  • Plp
  • C3
  • C3-1
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
  • C3d
  • asp
  • cpamd1
  • c3
  • complement C3
  • complement component 3
  • complement component c3a, duplicate 1
  • complement component 3 L homeolog
  • complement component C3
  • complement C3-like
  • C3
  • c3a.1
  • c3.L
  • LOC443475
  • c3
  • c3-1
  • LOC100060539
Reactivity
Guinea Pig, Human, Rabbit
290
80
71
16
15
8
7
6
5
3
3
1
Host
Mouse
165
133
97
27
14
8
Clonality
Monoclonal
Conjugate
This C3 antibody is un-conjugated
38
37
23
7
7
6
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
227
206
100
76
68
61
56
51
45
39
38
31
17
11
5
5
5
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN215710
$ 969.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN111064 EIA IHC (fro) WB Mouse IgG2b Log in to see H13 3

General

Antigen Complement Component 3 (C3) Antibodies
Reactivity Guinea Pig, Human, Rabbit
(290), (80), (71), (16), (15), (8), (7), (6), (5), (3), (3), (1)
Host Mouse
(165), (133), (97), (27), (14), (8)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C3 antibody is un-conjugated
(38), (37), (23), (7), (7), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(227), (206), (100), (76), (68), (61), (56), (51), (45), (39), (38), (31), (17), (11), (5), (5), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling Images
Specificity Recognizes a C3a-determinant (C3 alpha-chain) which is present also on native C3. Allows discrimination of ubiquitous or nonspecifically bound native C3 from biologically active C3b fragment, C3b or C are negative with this clone. The antibody inhibits the anaphylactic function of C3a: the capacity of C3a to induce serotonin release from thrombocytes in vitro is inhibited, similarly, treatment of C3a with the antibody inhibits the C3a-induced skin reaction in vivo. Species cross-reactivity: Guinea pig and rabbit C3/C3a.
Purification Protein A purified
Immunogen Human complement component C3.

Type of Immunogen: Purified protein
Clone H13
Isotype IgG2b
Plasmids, Primers & others

Target Details C3

Product Details anti-C3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background Name/Gene ID: C3

Synonyms: C3, AHUS5, ARMD9, Complement component C3, CPAMD1, Complement C3, ASP, Complement component 3
Gene ID 718
UniProt P01024
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling Images back to top
Application Notes Approved: IHC, IHC-Fr, WB

Usage: Suitable for use in Immunohistochemistry (frozen sections) and Western blotting. The antibody is, in combination with antibodies to the C3b fragment, an excellent control for distinguishing ubiquitous or nonspecifically bound native C3 from the biologically active C3 fragment. Nonactivated C3 still bears the C3a determinant, whereas activated C3 (C3b, C3d) is C3a-negative. It is therefore useful for immunopathological assays (demonstration of C3 deposition in biopsies) and for C3a assays research purposes.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details Images back to top
Format Lyophilized
Reconstitution 1 mL sterile ddH20
Concentration Lot specific
Buffer Lyophilized from PBS,  pH 7.4, 0.5 % BSA, 0.9 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Lyophilized and resuspended forms: store at -20°C.