anti-Mouse (Murine) CD55 antibody for Flow Cytometry

Recommended CD55 Antibody (supplied by: Log in to see )

Antigen
Complement Decay-Accelerating Factor (CD55) Antibodies
  • CR
  • CROM
  • DAF
  • TC
  • Daf
  • Daf-GPI
  • Daf1
  • GPI-DAF
  • CD55 molecule (Cromer blood group)
  • CD55 molecule, decay accelerating factor for complement
  • CD55
  • Cd55
Reactivity
Mouse (Murine)
462
55
24
11
9
4
3
2
2
2
Host
Rat
370
117
14
10
8
3
3
Clonality
Monoclonal
Conjugate
This CD55 antibody is conjugated to FITC
57
50
32
19
9
9
8
8
8
7
5
5
5
5
5
5
5
5
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
376
201
121
106
74
73
44
43
16
13
6
6
6
5
3
2
1
1
1
1
1
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Catalog No. ABIN2191854
$ 680.92
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
17.575203 ABIN2192214 FACS WB Rat Log in to see 3D5 4
17.004213 ABIN4900282 CyTOF FACS WB Sheep IgG AA 35-359 Log in to see Polyclonal 1
15.504213 ABIN4899086 CyTOF FACS WB Rat IgG2a AA 1-359 Log in to see 583905 0
13.075204 ABIN1112474 FACS IF PE Armenian Hamster IgG Log in to see RIKO-3 0
13.075204 ABIN6150949 FACS Armenian Hamster IgG Log in to see RIKO-3 0
13.075204 ABIN161406 FACS ELISA WB Hamster IgG Log in to see RIK0-3 0
12.504213 ABIN5506309 FACS IF IF/ICC Rabbit IgG AA 1-361 Log in to see 076 0
9.504213 ABIN951354 EIA FACS WB Hamster IgG Log in to see RIKO-3 1
9.504213 ABIN2213417 FACS APC Rabbit IgG AA 1-361 Log in to see 076 0
9.504213 ABIN2213419 FACS FITC Rabbit IgG AA 1-361 Log in to see 076 0
9.504213 ABIN2691682 FACS PerCP Rabbit IgG AA 1-361 Log in to see 076 0
9.504213 ABIN2213421 FACS PE Rabbit IgG AA 1-361 Log in to see 076 0
9.504213 ABIN951353 EIA FACS WB Biotin Hamster IgG Log in to see RIKO-3 0
9.504213 ABIN2663530 FACS PE Armenian Hamster IgG Log in to see RIKO-3 1
9.504213 ABIN2657825 FACS Alexa Fluor 647 Armenian Hamster IgG Log in to see RIKO-3 1
1 ABIN308032 FACS FITC Hamster IgG Log in to see RIKO-3 0
1 ABIN308031 FACS ELISA WB Biotin Hamster IgG Log in to see RIKO-3 0
1 ABIN308033 FACS PE Hamster IgG Log in to see RIKO-3 0
1 ABIN212859 FACS ELISA WB Armenian Hamster IgG Log in to see RIKO-3 0
1 ABIN1821173 FACS Hamster IgG Log in to see G1-G1-4 0

General

Antigen Complement Decay-Accelerating Factor (CD55) Antibodies
Reactivity Mouse (Murine)
(462), (55), (24), (11), (9), (4), (3), (2), (2), (2)
Host Rat
(370), (117), (14), (10), (8), (3), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD55 antibody is conjugated to FITC
(57), (50), (32), (19), (9), (9), (8), (8), (8), (7), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Western Blotting (WB)
(376), (201), (121), (106), (74), (73), (44), (43), (16), (13), (6), (6), (6), (5), (3), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD55 Antibody

Target Details CD55 Application Details Handling References for anti-CD55 antibody (ABIN2191854) Images
Sterility 0.2 μm filtered
Clone 3D5
Plasmids, Primers & others

Target Details CD55

Product Details anti-CD55 Antibody Application Details Handling References for anti-CD55 antibody (ABIN2191854) Images back to top
Antigen
Alternative Name Complement Decay Accelerating Factor (CD55 Antibody Abstract)
Background The monoclonal antibody 3D5 recognizes complement decay accelerating factor (DAF), also designated as CD55. Cells express on their surface several proteins which protect against complement attack, namely C receptor I (CR1), decay accelerating factor (DAF), membrane cofactor protein (MCP) and CD59. CR1, DAF and MCP regulate the activation pathways of complement by either accelerating decay of the C3 and C5 convertase (CR1, DAF), or acting as cofactors for the serine protease factor I, which cleaves and irreversibly inactivates C3b (CR1, MCP). Mouse DAF (CD55) is a 60 kDa transmembrane protein that binds C3b and C4b to inhibit formation and half-life of the C3 convertases. DAF is broadly distributed among cells in contact with serum, including both haematopoietic and nonhaematopoietic cells. Although DAF does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells. Together with other complement regulators DAF protects self cells from autologous complement- mediated injury. DAF cooperates with CD46 in circumventing autologous C3 deposition, while CD59 inhibits the pathway at the critical end-point. Aliases DAF (decay accelerating factor), CD55 Immunogen NRK cells expressing transmembrane-anchored mouse DAF
Pathways Complement System, Regulation of Leukocyte Mediated Immunity

Application Details

Product Details anti-CD55 Antibody Target Details CD55 Handling References for anti-CD55 antibody (ABIN2191854) Images back to top
Application Notes For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Restrictions For Research Use only

Handling

Product Details anti-CD55 Antibody Target Details CD55 Application Details References for anti-CD55 antibody (ABIN2191854) Images back to top
Buffer PBS, containing 1.0 % bovine serum albumin and 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Expiry Date 12 months

References for anti-CD55 antibody (ABIN2191854)

Product Details anti-CD55 Antibody Target Details CD55 Application Details Handling Images back to top
Product cited in:

Lin, Fukuoka, Spicer, Ohta, Okada, Harris, Emancipator, Medof: "Tissue distribution of products of the mouse decay-accelerating factor (DAF) genes. Exploitation of a Daf1 knock-out mouse and site-specific monoclonal antibodies." in: Immunology, Vol. 104, Issue 2, pp. 215-25, 2001

Harris, Spiller, Morgan: "Human and rodent decay-accelerating factors (CD55) are not species restricted in their complement-inhibiting activities." in: Immunology, Vol. 100, Issue 4, pp. 462-70, 2000

Spiller, Goodfellow, Evans, Almond, Morgan: "Echoviruses and coxsackie B viruses that use human decay-accelerating factor (DAF) as a receptor do not bind the rodent analogues of DAF." in: The Journal of infectious diseases, Vol. 181, Issue 1, pp. 340-3, 2000

Spiller, Harris, Morgan: "Efficient generation of monoclonal antibodies against surface-expressed proteins by hyperexpression in rodent cells." in: Journal of immunological methods, Vol. 224, Issue 1-2, pp. 51-60, 1999