anti-Human Complement Factor H antibody for Western Blotting

Recommended Complement Factor H Antibody (supplied by: Log in to see )

Antigen
Complement Factor H (CFH) Antibodies
  • AHUS1
  • AMBP1
  • ARMD4
  • ARMS1
  • CFHL3
  • FH
  • FHL1
  • HF
  • HF1
  • HF2
  • HUS
  • Mud-1
  • NOM
  • Sas-1
  • Sas1
  • Fh
  • CFH
  • im:7147412
  • si:ch211-207o17.2
  • fH
  • armd4
  • arms1
  • cfh
  • cfhl3
  • fhl1
  • hf1
  • hf2
  • hus
  • complement factor H
  • complement component factor h
  • complement factor H-related protein 5
  • complement factor H L homeolog
  • CFH
  • Cfh
  • cfh
  • LOC100399888
  • LOC100733902
  • cfh.L
Reactivity
Mouse (Murine), Rat (Rattus), Human
266
36
36
4
4
3
3
2
2
Host
Rabbit
131
113
31
19
2
1
Clonality
Polyclonal
Conjugate
This Complement Factor H antibody is un-conjugated
24
17
8
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
216
157
77
41
39
27
26
24
15
11
11
10
10
5
4
2
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN3023097
$ 393.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
22.39519 ABIN2854706 IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal 0
19.8156 ABIN654896 IF IHC (p) WB Rabbit Ig Fraction AA 751-780, Center Log in to see Polyclonal 2
19.8156 ABIN1173336 ICC IHC WB Rabbit IgG AA 860-1231 Log in to see Polyclonal 0
19.8156 ABIN2896777 IF/ICC IHC IP WB Rabbit AA 860-1231 Log in to see Polyclonal 0
16.39519 ABIN185437 ELISA IHC WB Goat Internal Region Log in to see Polyclonal 1
12.579589 ABIN5621920 WB Rabbit IgG Log in to see Polyclonal 0
12.579589 ABIN2138054 ELISA WB Mouse IgG1 kappa Log in to see 34F12 0
12.579589 ABIN285281 ID ELISA WB Goat Log in to see Polyclonal 0
12.579589 ABIN2137491 ELISA WB Rabbit IgG Log in to see Polyclonal 0
12.579589 ABIN285280 ID ODD WB Sheep Log in to see Polyclonal 0
12.579589 ABIN6133652 IF IHC WB Rabbit Log in to see Polyclonal 0
12.579589 ABIN6138452 WB Rabbit Log in to see Polyclonal 0
10.8156 ABIN951646 EIA IF IHC (p) WB Rabbit Ig Fraction AA 751-780, Middle Region Log in to see Polyclonal 3
10.8156 ABIN6260777 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
10.8156 ABIN1871804 IHC WB Rabbit IgG Log in to see Polyclonal 0
10.8156 ABIN2995930 IF/ICC IHC WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN462334 ELISA IHC IHC (p) IP WB Mouse IgG1 Log in to see OX-24 0
7.8156 ABIN516404 WB Rabbit AA 1-449, full length Log in to see Polyclonal 0
7 ABIN337307 ELISA IHC IHC (p) WB Goat AA 577-588 Log in to see Polyclonal 0
7 ABIN364283 ELISA FACS IHC IHC (p) IP WB Mouse IgG1 Log in to see OX-24 0

General

Antigen Complement Factor H (CFH) Antibodies
Reactivity Mouse (Murine), Rat (Rattus), Human
(266), (36), (36), (4), (4), (3), (3), (2), (2)
Host Rabbit
(131), (113), (31), (19), (2), (1)
Clonality
Polyclonal
Conjugate This Complement Factor H antibody is un-conjugated
(24), (17), (8), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(216), (157), (77), (41), (39), (27), (26), (24), (15), (11), (11), (10), (10), (5), (4), (2), (1), (1), (1), (1), (1)
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Product Details anti-Complement Factor H Antibody

Target Details Complement Factor H Application Details Handling Images
Purification Affinity purification
Immunogen Recombinant protein of human CFH
Isotype IgG
Plasmids, Primers & others

Target Details Complement Factor H

Product Details anti-Complement Factor H Antibody Application Details Handling Images back to top
Antigen
Alternative Name CFH (CFH Antibody Abstract)
Background CFH (Complement factor H) is a member of the Regulator of Complement Activation (RCA) gene cluster and a protein with twenty short concensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this CFH have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. 1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1, 33:D54-8.
Synonyms: ARMD4, ARMS1, CFHL3, FH, FHL1, HF, HF1, HF2, HUS, MGC88246
Gene ID 3075
UniProt P08603
Pathways Complement System, Cellular Response to Molecule of Bacterial Origin

Application Details

Product Details anti-Complement Factor H Antibody Target Details Complement Factor H Handling Images back to top
Application Notes Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
Restrictions For Research Use only

Handling

Product Details anti-Complement Factor H Antibody Target Details Complement Factor H Application Details Images back to top
Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze / thaw cycles
Storage -20 °C/-80 °C

Images

Product Details anti-Complement Factor H Antibody Target Details Complement Factor H Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Complement Factor H (CFH) antibody (ABIN3023097) Western blot analysis of extracts of HepG2 cell line, using CFH antibody.
'Independent Validation' Badge
Antigen Commplement Factor H
Lot Number 4390101
Method validated Immunofluorescence
Positive Control MRL/lpr lupus mice (14 weeks of age)
Negative Control FH knockout MRL/lpr lupus mice
Notes Passed. The complement factor H antibody ABIN3023097 works very well on murine kidney samples and shows the expected CFH localization and strong staining.
Primary Antibody ABIN3023097
Secondary Antibody goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)
Protocol
  • Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
  • Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
  • Sections were cut at 8µm using the cryostat.
  • Dry sections for 30min at RT.
  • Hydrate sections in PBS 2x for 15min at RT.
  • Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
  • Wash sections 2x with PBS for 15min at RT.
  • Incubate sections with primary rabbit anti-Complement Factor H antibody (antibodies-online, ABIN3023097, 0004390101) diluted 1:100 in PBS ON at 4°C.
  • Wash sections 2x for 15min with PBS.
  • Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
  • Wash sections 2x for 15min with PBS.
  • Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
  • Wash sections 2x for 15min with PBS.
  • Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).
Validation Images
Immunofluorescence validation image for anti-Complement Factor H (CFH) antibody (ABIN3023097) IF staining with ABIN3023097 of kidneys harvested from 14 week old MRL/lpr mice suffi...