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|Antigen||Complement Factor H (CFH) Antibodies|
|Reactivity||Horse (Equine), Cat (Feline), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Complement Factor H antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunodiffusion (ID)
|1 reference available|
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Product Details anti-Complement Factor H AntibodyTarget Details Complement Factor H Application Details Handling References for anti-Complement Factor H antibody (ABIN113017) Images
|Specificity||Gives a single arc when tested by IEP against Human plasma. Identity is confirmed by double diffusion (Ouchterlony) vs. Human plasma and a known anti-Human Factor H. The cross reactivity of this product against the sera of the species listed below has been assessed by Double Diffusion using 10 μL of antisera against 10 μL of species sera. These results are intended as a guideline only. It is possible that cross-reactivity against species listed as negative above may be observed when using different test conditions or techniques.|
|Cross-Reactivity (Details)||Species reactivity (tested):Cat + Chicken - Cow - Dog + Guinea Pig + Horse + Human + Mouse + Pig - Rabbit - Rat + Goat -|
|Purification||Delipidation and Absorbtion to monospecificity by use of solid-phase absorbants. The titer is adjusted so that inter-batch variation is within 10%. Product is 0.2 µm filtered.|
|Immunogen||Human 155 kDa Factor H purified from plasma, shown to be pure by IEP.|
Target Details Complement Factor HProduct Details anti-Complement Factor H Antibody Application Details Handling References for anti-Complement Factor H antibody (ABIN113017) Images back to top
|Background||The complement Factor H protein is secreted into the bloodstream and acts in the regulation of complement activation. Mutations leading to changes in this protein have been linked with HUS (hemolytic-uremic syndrome) and chronic hypocomplementemic nephropathy.Synonyms: CFH, H factor 1, HF, HF1, HF2|
|Pathways||Complement System, Cellular Response to Molecule of Bacterial Origin|
Application DetailsProduct Details anti-Complement Factor H Antibody Target Details Complement Factor H Handling References for anti-Complement Factor H antibody (ABIN113017) Images back to top
Suitable for use in a variety of Gel techniques including Radial Immunodiffusion (RID),Double Diffusion and Immunoelectrophoresis (IEP). RID: 1 μL antiserum/cm2 gel vs.5 μL neat-1/10 dilution Human plasma. Double Diffusion: 10 μL antiserum vs.10 μL plasma. IEP: 100 μL antiserum vs.5 μL plasma. The use of 3 % PEG 6000 with 1.2 % agarose in a suitable buffer (such as TBE or tris-barbital pH > 8.2) is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Complement Factor H Antibody Target Details Complement Factor H Application Details References for anti-Complement Factor H antibody (ABIN113017) Images back to top
|Concentration||9.5 mg/mL (OD280 nm, E1% = 14.5)|
|Buffer||Glycine buffered saline, pH 7.4 with 0.09 % Sodium Azide, 0.1 % EACA, 0.01 % Benzamidine and 1 mM EDTA.|
|Preservative||Sodium azide, Benzamidine, EACA|
|Precaution of Use||This product contains Sodium azide, Benzamidine and EACA: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-Complement Factor H antibody (ABIN113017)Product Details anti-Complement Factor H Antibody Target Details Complement Factor H Application Details Handling Images back to top
Chung, Liszewski, Nybakken, Davis, Townsend, Fremont, Atkinson, Diamond: "West Nile virus nonstructural protein NS1 inhibits complement activation by binding the regulatory protein factor H." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 50, pp. 19111-6, 2006