Human Kallikrein 13 ELISA Kit

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Antigen
Kallikrein-Related Peptidase 13 (KLK13) ELISA Kits
  • KLK-L4
  • KLKL4
  • Egfbp-2
  • mGk-13
  • kallikrein related peptidase 13
  • kallikrein related-peptidase 13
  • KLK13
  • Klk13
Epitope
AA 17-277
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
9
6
Method Type
Sandwich ELISA
Detection Range
156-10.000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
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Catalog No. ABIN2859316
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN1874354 Sandwich ELISA Cell Lysate, Plasma, Serum, Tissue Homogenate 31.25 pg/mL - 2000 pg/mL 31.25 pg/mL Log in to see
1 ABIN1570914 Sandwich ELISA Cell Lysate, Plasma, Serum, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN842247 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate 12.5-800 ng/mL 12.5 ng/mL Log in to see
1 ABIN5656149 Sandwich ELISA Cell Lysate, Plasma, Serum, Tissue Homogenate 31.25 pg/mL - 2000 pg/mL 31.25 pg/mL Log in to see
1 ABIN4883529 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate Log in to see
1 ABIN2535750 Sandwich ELISA Cell Lysate, Plasma, Serum, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN4970604 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN5499274 Sandwich ELISA Recombinant Protein 31.25-2000 pg/mL 31.25 pg/mL Log in to see
1 ABIN2043839 Sandwich ELISA 0.15-10 ng/mL 0.15 ng/mL Log in to see

General

Antigen Kallikrein-Related Peptidase 13 (KLK13) ELISA Kits
Epitope AA 17-277
Reactivity Human
Kits with alternative reactivity to:
(9), (6)
Method Type Sandwich ELISA
Detection Range 156-10.000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Supplier Log in to see

Product Details Kallikrein 13 ELISA Kit

Target Details Application Details Handling Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human KLK13
Brand PicoKine™
Sample Type Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Analytical Method Quantitative
Specificity NSO, G17-Q277
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: G17-Q277

Target Details

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Antigen
Alternative Name KLK13 (KLK13 ELISA Kit Abstract)
Background

Background: Kallikrein-13 is a protein that in humans is encoded by the KLK13 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. By genomic sequence analysis, KLK13 gene is mapped in a 300-kb region on chromosome 19q13.3-q13.4. It has been shown that recombinant hK13 produced in yeast can cleave synthetic peptides after the arginine residue and some extracellular matrix components. However, its exact physiological substrates and functions remain obscure. Despite the lack of knowledge on the physiological function of hK13, several studies have demonstrated that hK13 is implicated with cancer of the breast and ovary and it can serve as a favorable prognostic biomarker for these malignancies.

Synonyms: Kallikrein-13,3.4.21.-,Kallikrein-like protein 4,KLK-L4,KLK13,KLKL4,

Full Gene Name: Kallikrein-13

Cellular Localisation: Secreted.
Gene ID 26085
UniProt Q9UKR3
Pathways Complement System

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Tissue Specificity: Expressed in prostate, breast, testis and salivary gland.

Plate Pre-coated
Protocol human KLK13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK13 has been precoated onto 96-well plates. Standards (NSO,G17-Q277) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK13 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human KLK13 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 1661, Standard deviation: 83.05, CV(%): 5.0
  • Sample 2: n=16, Mean(pg/ml): 3572, Standard deviation: 196.46, CV(%): 5.5
  • Sample 3: n=16, Mean(pg/ml): 6985, Standard deviation: 405.13, CV(%): 5.8,
  • Sample 1: n=24, Mean(pg/ml): 1369, Standard deviation: 71.18, CV(%): 5.2
  • Sample 2: n=24, Mean(pg/ml): 4425, Standard deviation: 269.92, CV(%): 6.1
  • Sample 3: n=24, Mean(pg/ml): 6594, Standard deviation: 422.01, CV(%): 6.4
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

Images

Product Details Kallikrein 13 ELISA Kit Target Details Application Details Handling back to top
Supplier Images
ELISA image for Kallikrein-Related Peptidase 13 (KLK13) ELISA Kit (ABIN2859316) Human KLK13 PicoKine ELISA Kit standard curve