anti-Human Prothrombin antibody for Enzyme Immunoassay

Recommended Prothrombin Antibody (supplied by: Log in to see )

Antigen
Coagulation Factor II (thrombin) (F2) Antibodies
  • PT
  • RPRGL2
  • THPH1
  • Cf-2
  • Cf2
  • FII
  • thrombin
  • zgc:66319
  • wu:fb57c10
  • wu:fd42e09
  • wu:fd59d01
  • prothrombin
  • F2
  • coagulation factor II, thrombin
  • coagulation factor II
  • coagulation factor II (thrombin)
  • F2
  • f2
Reactivity
Human
196
81
47
9
7
4
2
2
2
2
1
Host
Mouse
107
92
56
8
6
4
2
Clonality
Monoclonal
Conjugate
This Prothrombin antibody is un-conjugated
28
16
15
5
5
4
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
185
131
54
20
18
18
18
18
15
14
14
13
6
3
3
2
1
1
1
1
1
1
1
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Pubmed
3 references available
Catalog No. ABIN954323
$ 577.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN954322 EIA WB Biotin Mouse IgG1 Log in to see CaPro-20 3
1 ABIN264058 EIA WB Mouse IgG2a Log in to see BDI095 0
1 ABIN5568046 EIA RIA Biotin Rabbit AA 328-622 Log in to see Polyclonal 0
1 ABIN5568043 EIA RIA Rabbit AA 328-622 Log in to see Polyclonal 0
1 ABIN5586252 EIA Rabbit Log in to see Polyclonal 0
1 ABIN264120 EIA Mouse IgG1 Log in to see BDI905 0
1 ABIN613351 EIA RIA Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN613311 EIA RIA Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN613785 EIA RIA Rabbit IgG Log in to see Polyclonal 0
1 ABIN613825 EIA RIA Rabbit IgG Log in to see Polyclonal 0
1 ABIN613309 EIA RIA Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN613783 EIA RIA Rabbit IgG Log in to see Polyclonal 0

General

Antigen Coagulation Factor II (thrombin) (F2) Antibodies
Reactivity Human
(196), (81), (47), (9), (7), (4), (2), (2), (2), (2), (1)
Host Mouse
(107), (92), (56), (8), (6), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Prothrombin antibody is un-conjugated
(28), (16), (15), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(185), (131), (54), (20), (18), (18), (18), (18), (15), (14), (14), (13), (6), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Prothrombin Antibody

Target Details Prothrombin Application Details Handling References for anti-Prothrombin antibody (ABIN954323) Images
Specificity This antibody is specific for Human Prothrombin (CaPro-20).
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Protein G Chromatography of Ascitic fluid.
Immunogen Human Prothrombin (CaPro-20) purified from plasma.
Clone CaPro-20
Isotype IgG1
Plasmids, Primers & others

Target Details Prothrombin

Product Details anti-Prothrombin Antibody Application Details Handling References for anti-Prothrombin antibody (ABIN954323) Images back to top
Antigen
Alternative Name Prothrombin (F2) (F2 Antibody Abstract)
Background Prothrombin (Factor 2 in the coagulation cascade) is proteolytically cleaved at two sites by Factor Xa to form thrombin (Factor IIa) in an important step of the coagulation cascade which ultimately results in the stemming of blood loss. Thrombin being the single most important constituent of the coagulation cascade in terms of its feedback activation roles. Prothrombin also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in Prothrombin lead to various forms of thrombosis and dysprothrombinemia. Prothrombin is a 72 kDa blood clotting glycoprotein formed by and stored in the Liver. Prothrombin is present in the blood plasma and is converted to the key blood clotting agent thrombin, when blood vessels are damaged. Factor VII, another important haemostasis protein can be converted to VIIa by Factor IXa or Thrombin.Synonyms: Coagulation factor II
Gene ID 2147
NCBI Accession NP_000497
UniProt P00734
Pathways Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-Prothrombin Antibody Target Details Prothrombin Handling References for anti-Prothrombin antibody (ABIN954323) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Prothrombin Antibody Target Details Prothrombin Application Details References for anti-Prothrombin antibody (ABIN954323) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Prothrombin antibody (ABIN954323)

Product Details anti-Prothrombin Antibody Target Details Prothrombin Application Details Handling Images back to top
Background publications

Bharadwaj, Iino, Kontoyianni, Smith, Foster, Kisiel: "Factor VII central. A novel mutation in the catalytic domain that reduces tissue factor binding, impairs activation by factor Xa, and abolishes amidolytic and coagulant activity." in: The Journal of biological chemistry, Vol. 271, Issue 48, pp. 30685-91, 1997

Kazama, Pastuszyn, Wildgoose, Hamamoto, Kisiel: "Isolation and characterization of proteolytic fragments of human factor VIIa which inhibit the tissue factor-enhanced amidolytic activity of factor VIIa." in: The Journal of biological chemistry, Vol. 268, Issue 22, pp. 16231-40, 1993

Yamamoto, Nakagaki, Kisiel: "Tissue factor-dependent autoactivation of human blood coagulation factor VII." in: The Journal of biological chemistry, Vol. 267, Issue 27, pp. 19089-94, 1992