anti-Human Family with Sequence Similarity 175, Member A antibody for Flow Cytometry

Recommended Family with Sequence Similarity 175, Member A Antibody (supplied by: Log in to see )

Antigen
Family with Sequence Similarity 175, Member A (FAM175A) Antibodies
  • CCDC98
  • abra1
  • ccdc98
  • fam175a
  • ABRA1
  • 3830405G04Rik
  • 5630400M01Rik
  • AI506069
  • AL024423
  • AV118690
  • Ccdc98
  • RGD1305287
  • zgc:103522
  • abraxas 1, BRCA1 A complex subunit
  • Coiled-coil domain-containing protein 98
  • BRCA1 A complex subunit
  • family with sequence similarity 175 member A
  • abraxas 2a, BRISC complex subunit
  • abraxas 1, BRCA1 A complex subunit S homeolog
  • ABRAXAS1
  • abraxas1
  • Abraxas1
  • FAM175A
  • abraxas2a
  • abraxas1.S
Epitope
AA 1-313
12
6
5
3
3
2
Reactivity
Human
55
26
20
4
2
2
1
1
1
1
Host
Mouse
47
9
Clonality
Monoclonal
Conjugate
Un-conjugated
3
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
41
22
17
16
16
13
8
4
1
1
Options
Supplier
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Available images

Catalog No. ABIN1302443
$ 428.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN574440 FACS IHC IHC (p) IP WB Mouse IgG AA 1-313 Log in to see ABRA1-01 0
4 ABIN487439 FACS IHC (p) IP WB Mouse IgG AA 1-313, N-Term Log in to see ABRA1-01 0
4 ABIN1723577 FACS IHC (p) IP WB Mouse IgG AA 1-313 Log in to see ABRA1-01 0
1 ABIN2220783 FACS IP IHC WB Mouse IgG AA 1-313 Log in to see ABRA1-01 0
1 ABIN3036036 FACS IP WB Mouse IgG AA 1-313 Log in to see 0
1 ABIN5577791 FACS WB Mouse IgG Log in to see ABRA1-01 0
1 ABIN3199301 FACS IP WB Mouse IgG N-Term Log in to see ABRA1-01 0
-9.573446 ABIN4277468 FACS IHC (p) IP WB Mouse IgG N-Term Log in to see ABRA1-01 0

General

Antigen Family with Sequence Similarity 175, Member A (FAM175A) Antibodies
Epitope AA 1-313
(12), (6), (5), (3), (3), (2)
Reactivity Human
(55), (26), (20), (4), (2), (2), (1), (1), (1), (1)
Host Mouse
(47), (9)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(41), (22), (17), (16), (16), (13), (8), (4), (1), (1)
Pubmed 3 references available
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Product details

Target Details Application Details Handling References Images
Specificity The mouse monoclonal antibody ABRA1-01 recognizes N-terminal part of ABRA1 (Abraxas, CCDC98), an adaptor protein involved in DNA repair, which migrates as a 45 kDa band on PAAGE under reducing conditions.
Cross-Reactivity (Details) Human, Other not tested
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Immunogen Recombinant protein corresponding to amino acids 1-313 of ABRA1 with N-terminal His6 tag
Clone ABRA1-01
Isotype IgG

Target Details

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Antigen
Alternative Name ABRA1 (FAM175A Antibody Abstract)
Background ABRA1 (Abraxas), also known as CCDC98, is an adaptor protein that is essential for formation and function of BRCA1 A tumor suppressor complex. This complex plays critical roles in DNA repair, cell cycle checkpoint control, and maintenance of genomic stability. ABRA1 mediates interaction of ubiquitin-interacting motif-containing protein RAP80 and deubiquitination enzyme BRCC36 with BRCA1/BARD1. ABRA1 controls both DNA-damage-induced formation of BRCA1 foci and BRCA1-dependent G2/M checkpoint activation.
Pathways DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus

Application Details

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Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

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Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References

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Background publications

Wang, Elledge: "Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 52, pp. 20759-63, 2007

Liu, Wu, Yu: "CCDC98 targets BRCA1 to DNA damage sites." in: Nature structural & molecular biology, Vol. 14, Issue 8, pp. 716-20, 2007

Wang, Matsuoka, Ballif, Zhang, Smogorzewska, Gygi, Elledge: "Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1194-8, 2007

Images

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Supplier Images
Western Blotting (WB) image for anti-Family with Sequence Similarity 175, Member A (FAM175A) (AA 1-313) antibody (ABIN1302443) Detection of ABRA1-EGFP fusion protein in lysate of transfected HEK293 cells. Lane A:...