anti-Human Family with Sequence Similarity 175, Member A antibody for Immunoprecipitation

Recommended Family with Sequence Similarity 175, Member A Antibody

Antigen
Family with Sequence Similarity 175, Member A (FAM175A) Antibodies
  • CCDC98
  • abra1
  • ccdc98
  • fam175a
  • ABRA1
  • 3830405G04Rik
  • 5630400M01Rik
  • AI506069
  • AL024423
  • AV118690
  • Ccdc98
  • RGD1305287
  • zgc:103522
  • abraxas 1, BRCA1 A complex subunit
  • Coiled-coil domain-containing protein 98
  • BRCA1 A complex subunit
  • family with sequence similarity 175 member A
  • abraxas 2a, BRISC complex subunit
  • abraxas 1, BRCA1 A complex subunit S homeolog
  • ABRAXAS1
  • abraxas1
  • Abraxas1
  • FAM175A
  • abraxas2a
  • abraxas1.S
Epitope
AA 1-313
10
6
5
2
2
2
Reactivity
Human
57
25
20
4
2
2
1
1
1
1
Host
Mouse
50
8
Clonality
Monoclonal
Conjugate
Un-conjugated
4
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
37
19
15
14
13
12
7
3
1
Options

Available images

Catalog No. ABIN1302443
$ 428.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
7 ABIN574440 FACS IHC IHC (p) IP WB Mouse IgG AA 1-313 ABRA1-01 0
4 ABIN487439 FACS IHC (p) IP WB Mouse IgG AA 1-313, N-Term ABRA1-01 0
4 ABIN2601274 IHC IHC (p) IP WB Rabbit IgG AA 350-400 Polyclonal 0
4 ABIN1723577 FACS IHC (p) IP WB Mouse IgG AA 1-313 ABRA1-01 0
1 ABIN2746339 ELISA IP WB Rabbit IgG N-Term Polyclonal 0
1 ABIN2220783 FACS IP IHC WB Mouse IgG AA 1-313 ABRA1-01 0
1 ABIN1894726 IF IHC IHC (p) IP Rabbit IgG AA 400-409 Polyclonal 0
1 ABIN2749550 ELISA IP WB FITC Rabbit IgG C-Term Polyclonal 0
1 ABIN2749548 ELISA IP WB Rabbit IgG C-Term Polyclonal 0
1 ABIN3036036 FACS IP WB Mouse IgG AA 1-313 0
1 ABIN3199301 FACS IP WB Mouse IgG N-Term ABRA1-01 0
1 ABIN2962713 IP IHC (p) WB Rabbit IgG AA 350-400 Polyclonal 0
1 ABIN5558585 ELISA IP WB Biotin Rabbit Polyclonal 0
-7.1118393 ABIN439660 IHC (p) IP IHC Rabbit AA 400-409 Polyclonal 0
-10.111839 ABIN4277468 FACS IHC (p) IP WB Mouse IgG N-Term ABRA1-01 0
-10.111839 ABIN439659 IP WB Rabbit AA 350-400 Polyclonal 0

General

Antigen Family with Sequence Similarity 175, Member A (FAM175A) Antibodies
Epitope AA 1-313
(10), (6), (5), (2), (2), (2)
Reactivity Human
(57), (25), (20), (4), (2), (2), (1), (1), (1), (1)
Host Mouse
(50), (8)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(37), (19), (15), (14), (13), (12), (7), (3), (1)
Pubmed 3 references available
Supplier

Product details

Target Details Application Details Handling References Images
Specificity The mouse monoclonal antibody ABRA1-01 recognizes N-terminal part of ABRA1 (Abraxas, CCDC98), an adaptor protein involved in DNA repair, which migrates as a 45 kDa band on PAAGE under reducing conditions.
Cross-Reactivity (Details) Human, Other not tested
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Immunogen Recombinant protein corresponding to amino acids 1-313 of ABRA1 with N-terminal His6 tag
Clone ABRA1-01
Isotype IgG

Target Details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name ABRA1 (FAM175A Antibody Abstract)
Background ABRA1 (Abraxas), also known as CCDC98, is an adaptor protein that is essential for formation and function of BRCA1 A tumor suppressor complex. This complex plays critical roles in DNA repair, cell cycle checkpoint control, and maintenance of genomic stability. ABRA1 mediates interaction of ubiquitin-interacting motif-containing protein RAP80 and deubiquitination enzyme BRCC36 with BRCA1/BARD1. ABRA1 controls both DNA-damage-induced formation of BRCA1 foci and BRCA1-dependent G2/M checkpoint activation.
Pathways DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus

Application Details

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Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

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Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References

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Background publications

Wang, Elledge: "Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 52, pp. 20759-63, 2007

Liu, Wu, Yu: "CCDC98 targets BRCA1 to DNA damage sites." in: Nature structural & molecular biology, Vol. 14, Issue 8, pp. 716-20, 2007

Wang, Matsuoka, Ballif, Zhang, Smogorzewska, Gygi, Elledge: "Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1194-8, 2007

Images

Product details Target Details Application Details Handling References back to top
Supplier Images
Western Blotting (WB) image for anti-Family with Sequence Similarity 175, Member A (FAM175A) (AA 1-313) antibody (ABIN1302443) Detection of ABRA1-EGFP fusion protein in lysate of transfected HEK293 cells. Lane A:...