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|Antigen||General Transcription Factor IIH, Polypeptide 1, 62kDa (GTF2H1) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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|Specificity||This antibody detects endogenous levels of TFIIH p62 protein. (region surrounding Ala32)|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Mouse.|
|Purification||Affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % (by SDS-PAGE)|
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|Alternative Name||GTF2H1 / BTF2 (GTF2H1 Antibody Abstract)|
|Background||Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription actors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFII. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and MAT1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.Synonyms: BTF2-p62, Basic transcription factor 2 62 kDa subunit, General transcription factor IIH polypeptide 1, General transcription factor IIH subunit 1, TFIIH, TFIIH basal transcription factor complex p62 subunit|
|Molecular Weight||approx. 57 kDa|
|Pathways||DNA Damage Repair|
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Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.|
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