anti-Rabbit Polymerase (DNA Directed) iota antibody for Western Blotting

Recommended Polymerase (DNA Directed) iota Antibody (supplied by: Log in to see )

Antigen
Polymerase (DNA Directed) iota (POLI) Antibodies
  • poli
  • wu:fc27h11
  • zgc:110185
  • GB12093
  • NV16187
  • RAD30B
  • RAD3OB
  • Rad30b
  • DNA polymerase iota
  • polymerase (DNA directed) iota
  • polymerase (DNA directed), iota
  • POLI
  • poli
  • LOC551219
  • AFUA_4G11140
  • LOC5568276
  • Pol-iota
  • Poli
Epitope
N-Term
19
14
10
7
4
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Yeast (Saccharomyces cerevisiae), Zebrafish (Danio rerio)
99
28
25
1
1
1
1
1
1
1
1
Host
Rabbit
75
24
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
81
38
36
13
10
5
5
5
1
Options
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Available images

Catalog No. ABIN2784869
$ 289.00
Plus shipping costs $45.00

General

Antigen Polymerase (DNA Directed) iota (POLI) Antibodies
Epitope N-Term
(19), (14), (10), (7), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Yeast (Saccharomyces cerevisiae), Zebrafish (Danio rerio)
(99), (28), (25), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(75), (24)
Clonality
Polyclonal
Conjugate Un-conjugated
(4), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(81), (38), (36), (13), (10), (5), (5), (5), (1)
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Product details

Target Details Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN981952
Sequence PVVERLGFDE NFVDLTEMVE KRLQQLQSDE LSAVTVSGHV YNNQSINLLD
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 82%, Zebrafish: 86%
Characteristics This is a rabbit polyclonal antibody against POLI. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human POLI

Target Details

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Antigen
Alternative Name POLI (POLI Antibody Abstract)
Background POLI is an error-prone DNA polymerase specifically involved in DNA repair.POLI plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls.POLI favors Hoogsteen base-pairing in the active site.POLI inserts the correct base with high-fidelity opposite an adenosine template.POLI exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine.POLI may play a role in hypermutation of immunogobulin genes.POLI forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
Alias Symbols: RAD30B, RAD3OB
Protein Interaction Partner: TMEM239, TRIM39, ZBTB44, UBC, TRAF6, DAZAP2, RNF11, TRIM23, POLH, PCNA, APP, REV1, XRCC1, UBB, UBA52, CALCOCO2, DVL2,
Protein Size: 740
Molecular Weight 83 kDa
Gene ID 11201
NCBI Accession NM_007195, NP_009126
UniProt Q9UNA4
Pathways DNA Damage Repair

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 740 AA

Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

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Supplier Images
Western Blotting (WB) image for anti-Polymerase (DNA Directed) iota (POLI) (N-Term) antibody (ABIN2784869) WB Suggested Anti-POLI Antibody Titration: 0.2-1 ug/mlPositive Control: Human Lung
Western Blotting (WB) image for anti-Polymerase (DNA Directed) iota (POLI) (N-Term) antibody (ABIN2784869) WB Suggested Anti-POLI Antibody Titration: 0.2-1 µg/mL Positive Control: Human Lung