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|Antigen||Ubiquitin Interaction Motif Containing 1 (UIMC1) Antibodies|
|Epitope||Internal Region Alternatives|
ELISA, Immunohistochemistry (IHC)
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|Blocking Peptide||Blocking peptide for this product available: ABIN2816353|
|Predicted Reactivity||Human, Mouse, Rat, Dog, Pig|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Immunogen||Peptide with sequence C-TRRQKEAKTKSDSG, from the internal region of the protein sequence according to NP_057374.3.|
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|Alternative Name||UIMC1 (UIMC1 Antibody Abstract)|
|Background||UIMC1, ubiquitin interaction motif containing 1, RAP80, X2HRIP110, OTTHUMP00000161441, receptor associated protein 80, retinoid x receptor interacting protein|
|Pathways||DNA Damage Repair, Nuclear Hormone Receptor Binding, Positive Regulation of Response to DNA Damage Stimulus|
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DS WB Results: Preliminary experiments gave an approx 60 kDa band in Human Heart and Spleen lysates and in lysates of cell lines A431, HeLa and HepG2 after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.7 kDa according to NP_057374.3. The 60 kDa band was successfully blocked by incubation with the immunizing peptide.
DS IHC Results: Paraffin embedded Human Testis. Recommended concentration: 3.75 μg/mL.
Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:32000.
|Restrictions||For Research Use only|
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|Buffer||Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Minimize freezing and thawing.|
|Storage Comment||Aliquot and store at -20 °C.|