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|Antigen||Ubiquitin Interaction Motif Containing 1 (UIMC1) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
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|Cross-Reactivity||Human, Mouse (Murine)|
|Cross-Reactivity (Details)||Cross reactivity with RAP80 from other sources has not been determined.|
|Purification||Anti-RAP80 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.|
Immunogen: Anti-RAP80 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide from near the N-terminus of human RAP80.
Immunogen Type: Peptide
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|Alternative Name||RAP80 (UIMC1 Antibody Abstract)|
Synonyms: RAP80 Antibody, RAP80, X2HRIP110, RAP80, RXRIP110, BRCA1-A complex subunit RAP80, Receptor-associated protein 80
Background: RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.
Gene Name: UIMC1
|Pathways||DNA Damage Repair, Nuclear Hormone Receptor Binding, Positive Regulation of Response to DNA Damage Stimulus|
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Immunohistochemistry Dilution: 2.5 μg/mL
Application Note: Anti-RAP80 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 80 kDa in Western Blots of specific cell lysates and tissues.
ELISA Dilution: 1:10,000
Western Blot Dilution: 1 μg/mL
IF Microscopy Dilution: 20 μg/mL
|Restrictions||For Research Use only|
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||RT,4 °C,-20 °C|
|Storage Comment||Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
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