anti-Mouse (Murine) Ubiquitin Interaction Motif Containing 1 antibody for Immunohistochemistry

Recommended Ubiquitin Interaction Motif Containing 1 Antibody (supplied by: Log in to see )

Antigen
Ubiquitin Interaction Motif Containing 1 (UIMC1) Antibodies
  • UIMC1
  • RAP80
  • X2HRIP110
  • 9430016E08Rik
  • D330018D10Rik
  • D630032M02Rik
  • RIP110
  • Rxrip110
  • RGD1307009
  • ubiquitin interaction motif containing 1
  • UIMC1
  • Uimc1
Epitope
N-Term
14
8
7
7
4
3
2
2
1
Reactivity
Mouse (Murine)
85
29
10
3
3
1
Host
Rabbit
65
17
3
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
1
1
1
Application
ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
65
44
30
22
20
6
5
4
3
3
1
1
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Supplier
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Available images

Catalog No. ABIN6657598
$ 394.90
Plus shipping costs $45.00 and $22.60 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10.008073 ABIN343912 ELISA IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
10.008073 ABIN343913 ELISA IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
10.008073 ABIN1030613 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 0
10.008073 ABIN1031531 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 0
4 ABIN1740580 IHC (p) IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2883052 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5698665 ELISA IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1887174 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN1888034 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN5879614 ELISA IF/ICC IHC WB Rabbit IgG Log in to see Polyclonal 0
-3.5727158 ABIN4349368 ELISA ICC IF IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal 0
-3.5727158 ABIN4349367 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0

General

Antigen Ubiquitin Interaction Motif Containing 1 (UIMC1) Antibodies
Epitope N-Term
(14), (8), (7), (7), (4), (3), (2), (2), (1)
Reactivity Mouse (Murine)
(85), (29), (10), (3), (3), (1)
Host Rabbit
(65), (17), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(2), (2), (2), (1), (1), (1)
Application ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
(65), (44), (30), (22), (20), (6), (5), (4), (3), (3), (1), (1)
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Product details

Target Details Application Details Handling Images
Cross-Reactivity Human, Mouse (Murine)
Cross-Reactivity (Details) Cross reactivity with RAP80 from other sources has not been determined.
Purification Anti-RAP80 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Immunogen

Immunogen: Anti-RAP80 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide from near the N-terminus of human RAP80.

Immunogen Type: Peptide

Isotype IgG

Target Details

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Antigen
Alternative Name RAP80 (UIMC1 Antibody Abstract)
Background

Synonyms: RAP80 Antibody, RAP80, X2HRIP110, RAP80, RXRIP110, BRCA1-A complex subunit RAP80, Receptor-associated protein 80

Background: RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.

Gene Name: UIMC1

Gene ID 51720
UniProt Q96RL1
Pathways DNA Damage Repair, Nuclear Hormone Receptor Binding, Positive Regulation of Response to DNA Damage Stimulus

Application Details

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Application Notes

Immunohistochemistry Dilution: 2.5 μg/mL

Application Note: Anti-RAP80 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 80 kDa in Western Blots of specific cell lysates and tissues.

ELISA Dilution: 1:10,000

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 20 μg/mL

Restrictions For Research Use only

Handling

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Format Liquid
Buffer

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Images

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Supplier Images
Immunofluorescence (IF) image for anti-Ubiquitin Interaction Motif Containing 1 (UIMC1) (N-Term) antibody (ABIN6657598) Immunofluorescence of RAP80 Antibody Immunofluorescence Microscopy of RAP80 antibody....
Western Blotting (WB) image for anti-Ubiquitin Interaction Motif Containing 1 (UIMC1) (N-Term) antibody (ABIN6657598) Western Blot of RAP80 Antibody Western Blot of RAP80 antibody. Lane A: 293 cell lysat...
Immunohistochemistry (IHC) image for anti-Ubiquitin Interaction Motif Containing 1 (UIMC1) (N-Term) antibody (ABIN6657598) Immunohistochemistry of RAP80 Antibody Immunohistochemistry of RAP80 antibody. Tissue...