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|Antigen||Adenylate Cyclase 1 (Brain) (ADCY1) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This ADCY1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Product Details anti-ADCY1 AntibodyTarget Details ADCY1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of A Cyclase I protein. (region surrounding Asp252)|
Species reactivity (expected):Mouse.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
|Plasmids, Primers & others|
Target Details ADCY1Product Details anti-ADCY1 Antibody Application Details Handling Images back to top
|Alternative Name||Adenylate Cyclase Type 1 (ADCY1 Antibody Abstract)|
|Background||Adenylyl cyclases function to convert ATP to cyclic AMP in response to activation by a variety of hormones, neurotransmitters and other regulatory molecules. Cyclic AMP, in turn, activates several other target molecules to control a broad range of diverse phenomena such as metabolism, gene transcription and memory. Adenylyl cyclases respond to receptor-initiated signals, mediated by the Gs and Gi heterotrimeric G proteins. The binding of an agonist to a Gs-coupled receptor catalyzes the exchange of GDP (bound to Galpha s) for GTP, the dissociation of GTP-Galpha s from Gbeta-gamma and Galpha s)-mediated activation of adenylyl cyclase. Adenylyl cyclases type I (AC I) and III (AC III) have distinct staining within the cell nucleus of rat brain sensory neurons. AC I is expressed in myenteric ganglia as two bands of 160 kDa and 185 kDa by SDS-PAGE. Ca2+ stimulation of AC I and AC III is mediated by calmodulin. Protein associated with Myc (PAM) is a very potent inhibitor of AC I. A decrease in endogenous PAM levels in HeLa cells modulate both basal and agonist stimulated cAMP accumulation.Synonyms: ADCY1, ATP pyrophosphate-lyase 1, Adenylate cyclase type I, Adenylyl cyclase 1, Ca(2+)/calmodulin-activated adenylyl cyclase|
|Molecular Weight||approx. 130 kDa|
|Pathways||EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma|
Application DetailsProduct Details anti-ADCY1 Antibody Target Details ADCY1 Handling Images back to top
Western Blot: 1/500- 1/1000. Immuohistochemistry: 1/50- 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ADCY1 Antibody Target Details ADCY1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-ADCY1 Antibody Target Details ADCY1 Application Details Handling back to top