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|Antigen||Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT) Antibodies|
|Epitope||N-Term, AA 1-50 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This AGT antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
|6 references available|
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Product Details anti-AGT AntibodyTarget Details AGT Application Details Handling References for anti-AGT antibody (ABIN447446) Images
|Purification||Immunogen affinity purified|
|Immunogen||A synthetic peptide made to an N-terminal portion of the human Angiotensinogen protein (between residues 1-50) [UniProt P01019]|
Target Details AGTProduct Details anti-AGT Antibody Application Details Handling References for anti-AGT antibody (ABIN447446) Images back to top
|Alternative Name||Serpin A8/Angiotensinogen (AGT Antibody Abstract)|
|Background||Gene Symbol: AGT|
|Molecular Weight||Theoretical MW: 53 kDa|
|Pathways||JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Monocarboxylic Acid Catabolic Process, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Regulation of long-term Neuronal Synaptic Plasticity|
Application DetailsProduct Details anti-AGT Antibody Target Details AGT Handling References for anti-AGT antibody (ABIN447446) Images back to top
|Application Notes||Western Blot 1:1000, Immunohistochemistry 1:40-1:100, Immunocytochemistry/Immunofluorescence 1:40-1:100, Immunohistochemistry-Paraffin 1:50-1:100This Angiotensinogen antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot, a band is seen ~53 kDa representing Angiotensinogen. In ICC/IF, cytoplasmic staining was observed in HepG2 cells. In IHC-P, secreted and cytoplasmic staining was observed in mouse kidney tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Western Blot Protocol specific for Angiotensin antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.Immunohistochemistry-Paraffin Embedded Sections Protocol specific for Angiotensin antibody Immunohistochemistry-Paraffin Embedded Sections ProtocolAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
|Restrictions||For Research Use only|
HandlingProduct Details anti-AGT Antibody Target Details AGT Application Details References for anti-AGT antibody (ABIN447446) Images back to top
PBS and 30 % Glycerol
Buffer contains: 0.05 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
References for anti-AGT antibody (ABIN447446)Product Details anti-AGT Antibody Target Details AGT Application Details Handling Images back to top
|Product cited in:||
Wu, Ma, Zhang, Wen, Wang, Hu, Liu, Lu, Chen, Ruan, Liu: "Lipid disorder and intrahepatic renin-angiotensin system activation synergistically contribute to non-alcoholic fatty liver disease." in: Liver international : official journal of the International Association for the Study of the Liver, Vol. 36, Issue 10, pp. 1525-34, 2016 (Sample species: Human). Further details: Western Blotting
Kim, Kim, Kang, Kim, Ko, Liu, Rosenwaks, Ku: "Induction of multiple ovulation via modulation of angiotensin II receptors in in vitro ovarian follicle culture models." in: Journal of tissue engineering and regenerative medicine, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting
Ishigami, Kino, Chen, Minegishi, Araki, Umemura, Abe, Sasaki, Yamana, Umemura et al.: "Identification of bona fide alternative renin transcripts expressed along cortical tubules and potential roles in promoting insulin resistance in vivo without significant plasma renin activity ..." in: Hypertension (Dallas, Tex. : 1979), Vol. 64, Issue 1, pp. 125-33, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Ahnstedt, Cao, Krause, Warfvinge, Säveland, Nilsson, Edvinsson: "Male-female differences in upregulation of vasoconstrictor responses in human cerebral arteries." in: PLoS ONE, Vol. 8, Issue 4, pp. e62698, 2013 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry
Shababi, Habibi, Ma, Glascock, Sowers, Lorson: "Partial restoration of cardio-vascular defects in a rescued severe model of spinal muscular atrophy." in: Journal of molecular and cellular cardiology, Vol. 52, Issue 5, pp. 1074-82, 2012 (Sample species: Mouse (Murine)). Further details: Western Blotting
Dimitrijevic, Rissler, Luts, Edvinsson: "Reduced expression of angiotensin II and angiotensin receptor type 1 and type 2 in resistance arteries from nasal lesions in granulomatosis with polyangiitis (Wegener's granulomatosis)." in: Scandinavian journal of rheumatology, Vol. 40, Issue 6, pp. 448-52, 2011 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry
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