anti-Chicken PKC alpha antibody for Intracellular Staining

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • pkc-alpha
  • pkcalpha
  • im:7139045
  • si:dkey-8l19.1
  • LOC100219195
  • AAG6
  • PKC-alpha
  • PKCA
  • PRKACA
  • AI875142
  • Pkca
  • PKRCA
  • protein kinase C alpha
  • protein kinase C, alpha L homeolog
  • protein kinase C, alpha
  • PRKCA
  • prkca.L
  • prkca
  • Prkca
Epitope
AA 270-427
36
20
18
17
16
16
15
14
12
10
7
6
5
5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
306
219
175
31
25
25
21
21
20
5
3
3
2
1
1
Host
Mouse
275
43
2
Clonality
Monoclonal
Conjugate
This PKC alpha antibody is conjugated to Alexa Fluor 488
14
13
10
7
6
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Staining (ICS)
261
151
136
69
56
50
32
32
26
23
13
4
4
4
2
1
1
1
Options
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Pubmed
4 references available
Catalog No. ABIN1176927
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General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Epitope AA 270-427
(36), (20), (18), (17), (16), (16), (15), (14), (12), (10), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
(306), (219), (175), (31), (25), (25), (21), (21), (20), (5), (3), (3), (2), (1), (1)
Host Mouse
(275), (43), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PKC alpha antibody is conjugated to Alexa Fluor 488
(14), (13), (10), (7), (6), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Staining (ICS)
(261), (151), (136), (69), (56), (50), (32), (32), (26), (23), (13), (4), (4), (4), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling References for anti-PKC alpha antibody (ABIN1176927) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human PKCalpha aa. 270-427 Recombinant Protein
Clone 3-PKCalpha
Isotype IgG2b, kappa
Plasmids, Primers & others

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling References for anti-PKC alpha antibody (ABIN1176927) Images back to top
Antigen
Alternative Name PKCa (PKCa Antibody Abstract)
Background The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes, such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. Conventional PKC (cPKC) subfamily members (a, b, and gamma isoforms) consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the the C2-like domains of novel PKC (nPKC) subfamily members (delta, epsilon, eta, and theta isoforms) are Ca2+-independent. The atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) lack the C2 domain and are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC members. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. PKCalpha regulates a wide variety of functions such as cellular growth, apoptosis, cardiomyocyte function, and brain cognitive functions. The 3/PKCalpha monoclonal antibody recognizes PKCalpha, regardless of phosphorylation status, and has been reported to crossreact with PKCbeta.
Synonyms: Protein Kinase C alpha, PKC-alpha, PKC-A, PKCA, PRKCA
Pathways WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling References for anti-PKC alpha antibody (ABIN1176927) Images back to top
Application Notes Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details References for anti-PKC alpha antibody (ABIN1176927) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-PKC alpha antibody (ABIN1176927)

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling Images back to top
Product cited in:

Braz, Gregory, Pathak, Zhao, Sahin, Klevitsky, Kimball, Lorenz, Nairn, Liggett, Bodi, Wang, Schwartz, Lakatta, DePaoli-Roach, Robbins, Hewett, Bibb, Westfall, Kranias, Molkentin: "PKC-alpha regulates cardiac contractility and propensity toward heart failure." in: Nature medicine, Vol. 10, Issue 3, pp. 248-54, 2004

Parker, Murray-Rust: "PKC at a glance." in: Journal of cell science, Vol. 117, Issue Pt 2, pp. 131-2, 2003

Zhang, Bontrager, Hemler: "Transmembrane-4 superfamily proteins associate with activated protein kinase C (PKC) and link PKC to specific beta(1) integrins." in: The Journal of biological chemistry, Vol. 276, Issue 27, pp. 25005-13, 2001

Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." in: Nature, Vol. 334, Issue 6184, pp. 661-5, 1988