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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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21.292418 | ABIN2749044 | FACS | Biotin | Mouse | IgG2b | AA 1-444 | Log in to see | MEM-CD148-05 | 1 | |
14.358341 | ABIN4898524 | FACS | PE | Mouse | IgG1 | Log in to see | 143-41 | 2 | ||
14.358341 | ABIN4898523 | FACS | Fluorescein | Mouse | IgG1 | Log in to see | 143-41 | 1 | ||
12.434076 | ABIN1043031 | FACS | Mouse | IgG2b | AA 1-444 | Log in to see | MEM-CD148-05 | 1 | ||
12.434076 | ABIN1111109 | FACS | PE | Mouse | IgG2b | AA 1-444 | Log in to see | MEM-CD148-05 | 1 | |
9.858341 | ABIN2663041 | BR FACS IP ELISA | PE | Mouse | IgG1 kappa | Log in to see | A3 | 6 | ||
9.858341 | ABIN2664244 | CyTOF FACS | Mouse | IgG1 kappa | Log in to see | A3 | 6 | |||
8.5 | ABIN4900751 | CyTOF FACS IP WB | Mouse | IgG1 | Log in to see | 143-41 | 5 | |||
7 | ABIN474737 | FACS | FITC | Mouse | IgG1, kappa | Log in to see | A3 | 0 | ||
1 | ABIN1105887 | FACS | Mouse | IgG2b | AA 1-444, N-Term | Log in to see | MEM-CD148-05 | 1 | ||
1 | ABIN1928113 | FACS | PE | Mouse | IgG2b | AA 1-444 | Log in to see | MEM-CD148-05 | 0 | |
1 | ABIN1928114 | FACS | Mouse | IgG2b | AA 1-444 | Log in to see | MEM-CD148-05 | 0 | ||
1 | ABIN4900510 | FACS | Mouse | IgG2b | AA 997-1337 | Log in to see | 261922 | 0 | ||
1 | ABIN1449306 | FACS | PE | Mouse | IgG2b | AA 1-444, N-Term | Log in to see | MEM-CD148-05 | 1 | |
1 | ABIN1818471 | FACS | PE | Mouse | IgG2a | Log in to see | 255 | 0 | ||
1 | ABIN1818470 | FACS | FITC | Mouse | IgG2a | Log in to see | 255 | 0 | ||
1 | ABIN2222940 | FACS IP IHC WB | Mouse | IgG1 kappa | Log in to see | A3 | 0 | |||
1 | ABIN1818469 | FACS IP | Mouse | IgG2a | Log in to see | 255 | 0 | |||
1 | ABIN3036710 | FACS | Mouse | IgG2b | AA 1-444 | Log in to see | 0 | |||
1 | ABIN3199474 | FACS | Mouse | IgG2b | N-Term | Log in to see | MEM-CD148-05 | 0 |
General |
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Antigen | Protein tyrosine Phosphatase, Receptor Type, J (PTPRJ) Antibodies |
Epitope | AA 1-444 Alternatives |
Reactivity | Human Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This PTPRJ antibody is conjugated to APC Alternatives |
Application |
Flow Cytometry (FACS)
Alternatives
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1 reference available |
Supplier | Log in to see |
Product Details anti-PTPRJ AntibodyTarget Details PTPRJ Application Details Handling References for anti-PTPRJ antibody (ABIN2749107) Images |
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Specificity | The mouse monoclonal antibody MEM-CD148/05 recognizes CD148, a highly glycosylated up to 250 kDa receptor-like protein tyrosin phosphatase expressed mainly in lymphocytes, myeloid cells and epithelial cells. |
Cross-Reactivity (Details) | Human |
Immunogen | Human recombinant CD148 (amino acids 1-444) |
Clone | MEM-CD148-05 |
Isotype | IgG2b |
Plasmids, Primers & others | |
Target Details PTPRJProduct Details anti-PTPRJ Antibody Application Details Handling References for anti-PTPRJ antibody (ABIN2749107) Images back to top |
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Antigen | |
Alternative Name | CD148 (PTPRJ Antibody Abstract) |
Background | CD148 (also known as HPTP-eta or DEP-1) is a transmembrane protein tyrosin phosphatase containing eight fibronectin type III extracellular domains. This protein is known to inhibit transduction of mitogenic signals in non-hematopoietic cells (fibroblasts, epithelial cells), and signal transduction downstream of T cell receptor, however, it also augments immunoreceptor signaling in B cells and macrophages via dephosphorylating C-terminal tyrosine of Src-family tyrosine kinases. CD148 expression increases after in vitro activation of peripheral blood leucocytes. It can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation. In contrast, in mice the CD148 expression sharply drops through the double positive stage to the single positive thymocytes. |
Pathways | EGFR Signaling Pathway, Platelet-derived growth Factor Receptor Signaling |
Application DetailsProduct Details anti-PTPRJ Antibody Target Details PTPRJ Handling References for anti-PTPRJ antibody (ABIN2749107) Images back to top |
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Application Notes | The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests. |
Comment |
The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
Restrictions | For Research Use only |
HandlingProduct Details anti-PTPRJ Antibody Target Details PTPRJ Application Details References for anti-PTPRJ antibody (ABIN2749107) Images back to top |
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Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide. |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Storage | 4 °C |
Storage Comment | Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. |
References for anti-PTPRJ antibody (ABIN2749107)Product Details anti-PTPRJ Antibody Target Details PTPRJ Application Details Handling Images back to top |
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Background publications |
Stepanek, Kalina, Draber, Skopcova, Svojgr, Angelisova, Horejsi, Weiss, Brdicka: "Regulation of Src family kinases involved in T cell receptor signaling by protein-tyrosine phosphatase CD148." in: The Journal of biological chemistry, Vol. 286, Issue 25, pp. 22101-12, 2011 |
ImagesProduct Details anti-PTPRJ Antibody Target Details PTPRJ Application Details Handling References for anti-PTPRJ antibody (ABIN2749107) back to top |
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Supplier Images |