anti-Human PTPRJ antibody for Flow Cytometry

Recommended PTPRJ Antibody (supplied by: Log in to see )

Antigen
Protein tyrosine Phosphatase, Receptor Type, J (PTPRJ) Antibodies
  • DEP-1
  • PTPRJ
  • CD148
  • DEP1
  • HPTPeta
  • R-PTP-ETA
  • SCC1
  • AI450271
  • BET
  • Byp
  • PTPbeta2
  • Ptpb2
  • RPTPJ
  • Scc-1
  • Scc1
  • protein tyrosine phosphatase, receptor type, J
  • protein tyrosine phosphatase, receptor type J
  • Ptprj
  • PTPRJ
  • EUBREC_2762
Epitope
AA 1-444
15
14
6
3
3
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human
127
24
19
1
1
Host
Mouse
67
65
2
Clonality
Monoclonal
Conjugate
This PTPRJ antibody is conjugated to APC
12
7
6
5
4
4
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
59
55
36
32
19
13
11
11
9
5
3
1
1
Options
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Pubmed
1 reference available

Available images

Flow Cytometry (FACS) image for anti-Protein tyrosine Phosphatase, Receptor Type, J (PTPRJ) (AA 1-444) antibody (APC) (ABIN2749107)
Catalog No. ABIN2749107
$ 497.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.292418 ABIN2749044 FACS Biotin Mouse IgG2b AA 1-444 Log in to see MEM-CD148-05 1
14.358341 ABIN4898524 FACS PE Mouse IgG1 Log in to see 143-41 2
14.358341 ABIN4898523 FACS Fluorescein Mouse IgG1 Log in to see 143-41 1
12.434076 ABIN1043031 FACS Mouse IgG2b AA 1-444 Log in to see MEM-CD148-05 1
12.434076 ABIN1111109 FACS PE Mouse IgG2b AA 1-444 Log in to see MEM-CD148-05 1
9.858341 ABIN2663041 BR FACS IP ELISA PE Mouse IgG1 kappa Log in to see A3 6
9.858341 ABIN2664244 CyTOF FACS Mouse IgG1 kappa Log in to see A3 6
8.5 ABIN4900751 CyTOF FACS IP WB Mouse IgG1 Log in to see 143-41 5
7 ABIN474737 FACS FITC Mouse IgG1, kappa Log in to see A3 0
1 ABIN1105887 FACS Mouse IgG2b AA 1-444, N-Term Log in to see MEM-CD148-05 1
1 ABIN1928113 FACS PE Mouse IgG2b AA 1-444 Log in to see MEM-CD148-05 0
1 ABIN1928114 FACS Mouse IgG2b AA 1-444 Log in to see MEM-CD148-05 0
1 ABIN4900510 FACS Mouse IgG2b AA 997-1337 Log in to see 261922 0
1 ABIN1449306 FACS PE Mouse IgG2b AA 1-444, N-Term Log in to see MEM-CD148-05 1
1 ABIN1818471 FACS PE Mouse IgG2a Log in to see 255 0
1 ABIN1818470 FACS FITC Mouse IgG2a Log in to see 255 0
1 ABIN2222940 FACS IP IHC WB Mouse IgG1 kappa Log in to see A3 0
1 ABIN1818469 FACS IP Mouse IgG2a Log in to see 255 0
1 ABIN3036710 FACS Mouse IgG2b AA 1-444 Log in to see 0
1 ABIN3199474 FACS Mouse IgG2b N-Term Log in to see MEM-CD148-05 0

General

Antigen Protein tyrosine Phosphatase, Receptor Type, J (PTPRJ) Antibodies
Epitope AA 1-444
(15), (14), (6), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(127), (24), (19), (1), (1)
Host Mouse
(67), (65), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PTPRJ antibody is conjugated to APC
(12), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(59), (55), (36), (32), (19), (13), (11), (11), (9), (5), (3), (1), (1)
Pubmed 1 reference available
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Product Details anti-PTPRJ Antibody

Target Details PTPRJ Application Details Handling References for anti-PTPRJ antibody (ABIN2749107) Images
Specificity The mouse monoclonal antibody MEM-CD148/05 recognizes CD148, a highly glycosylated up to 250 kDa receptor-like protein tyrosin phosphatase expressed mainly in lymphocytes, myeloid cells and epithelial cells.
Cross-Reactivity (Details) Human
Immunogen Human recombinant CD148 (amino acids 1-444)
Clone MEM-CD148-05
Isotype IgG2b
Plasmids, Primers & others

Target Details PTPRJ

Product Details anti-PTPRJ Antibody Application Details Handling References for anti-PTPRJ antibody (ABIN2749107) Images back to top
Antigen
Alternative Name CD148 (PTPRJ Antibody Abstract)
Background CD148 (also known as HPTP-eta or DEP-1) is a transmembrane protein tyrosin phosphatase containing eight fibronectin type III extracellular domains. This protein is known to inhibit transduction of mitogenic signals in non-hematopoietic cells (fibroblasts, epithelial cells), and signal transduction downstream of T cell receptor, however, it also augments immunoreceptor signaling in B cells and macrophages via dephosphorylating C-terminal tyrosine of Src-family tyrosine kinases. CD148 expression increases after in vitro activation of peripheral blood leucocytes. It can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation. In contrast, in mice the CD148 expression sharply drops through the double positive stage to the single positive thymocytes.
Pathways EGFR Signaling Pathway, Platelet-derived growth Factor Receptor Signaling

Application Details

Product Details anti-PTPRJ Antibody Target Details PTPRJ Handling References for anti-PTPRJ antibody (ABIN2749107) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Restrictions For Research Use only

Handling

Product Details anti-PTPRJ Antibody Target Details PTPRJ Application Details References for anti-PTPRJ antibody (ABIN2749107) Images back to top
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-PTPRJ antibody (ABIN2749107)

Product Details anti-PTPRJ Antibody Target Details PTPRJ Application Details Handling Images back to top
Background publications

Stepanek, Kalina, Draber, Skopcova, Svojgr, Angelisova, Horejsi, Weiss, Brdicka: "Regulation of Src family kinases involved in T cell receptor signaling by protein-tyrosine phosphatase CD148." in: The Journal of biological chemistry, Vol. 286, Issue 25, pp. 22101-12, 2011

Images

Product Details anti-PTPRJ Antibody Target Details PTPRJ Application Details Handling References for anti-PTPRJ antibody (ABIN2749107) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Protein tyrosine Phosphatase, Receptor Type, J (PTPRJ) (AA 1-444) antibody (APC) (ABIN2749107) Surface staining of human peripheral blood using anti-CD148 (MEM-CD148/05) APC.