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|Antigen||Signal Transducing Adaptor Molecule (SH3 Domain and ITAM Motif) 1 (STAM) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Human, Rat (Rattus), Mouse (Murine) Alternatives|
|Conjugate||This STAM antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-STAM AntibodyTarget Details STAM Application Details Handling References for anti-STAM antibody (ABIN350877) Images
|Specificity||Specific for STAM1 & 2.|
|Immunogen||A synthetic peptide from the internal region of human STAM 1, 2 (STAM-1, STAM-2) conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many species including rat, mouse, zebra fish and xenopus.|
|Plasmids, Primers & others|
Target Details STAMProduct Details anti-STAM Antibody Application Details Handling References for anti-STAM antibody (ABIN350877) Images back to top
|Alternative Name||STAM (STAM Antibody Abstract)|
Function: Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and c-myc induction. May also play a role in endocytic trafficking as well as in T-cell development.
Subunit: Heterodimer. Composed of STAM1 and HGS/HRS. Also interacts directly with STAMBP/AMSH.
Tissue specificity: Ubiquitously expressed. STAM2:
Function: Involved in intracellular signal transduction mediated by cytokines and growth factors. Probably acts via its association with ubiquitinated cargo proteins on the early endosome participates in their sorting for trafficking to the lysosome. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and c-myc induction. May also play a in T-cell development.
Subunit: Heterodimer or heterotrimer. At least composed of STAM2 and HGS/Hrs. EPS15 can be part of the complex. Interacts directly with STAMBP/AMSH, JAK2 and JAK3. Also interacts with ubiquitinated proteins and the deubiquitinating enzyme USP8/UBPY. Interacts directly with CBX5 via the PxVxL motif. Interacts with VPS37C.
Tissue specificity: Ubiquitously expressed. Also known as: STAM1, STAM-1, STAM2, STAM-2, Signal transducing adapter molecule 1, Signal transducing adapter molecule 2, HBP, Hrs-binding protein.
|Pathways||EGFR Signaling Pathway, EGFR Downregulation|
Application DetailsProduct Details anti-STAM Antibody Target Details STAM Handling References for anti-STAM antibody (ABIN350877) Images back to top
A dilution of 1: 300 to 1: 2000 is recommended.
The optimal dilution should be determined by the end user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAM Antibody Target Details STAM Application Details References for anti-STAM antibody (ABIN350877) Images back to top
|Reconstitution||Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.|
|Handling Advice||Avoid freeze and thaw cycles.|
|Storage||4 °C/-20 °C|
|Storage Comment||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Expiry Date||12 months|
References for anti-STAM antibody (ABIN350877)Product Details anti-STAM Antibody Target Details STAM Application Details Handling Images back to top
Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006
Hillier, Graves, Fulton, Fulton, Pepin, Minx, Wagner-McPherson, Layman, Wylie, Sekhon, Becker, Fewell, Delehaunty, Miner, Nash, Kremitzki, Oddy, Du, Sun, Bradshaw-Cordum, Ali, Carter, Cordes, Harris et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4. ..." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005
Steen, Kuster, Fernandez, Pandey, Mann: "Tyrosine phosphorylation mapping of the epidermal growth factor receptor signaling pathway." in: The Journal of biological chemistry, Vol. 277, Issue 2, pp. 1031-9, 2002
Asao, Sasaki, Arita, Tanaka, Endo, Kasai, Takeshita, Endo, Fujita, Sugamura: "Hrs is associated with STAM, a signal-transducing adaptor molecule. Its suppressive effect on cytokine-induced cell growth." in: The Journal of biological chemistry, Vol. 272, Issue 52, pp. 32785-91, 1998
Takeshita, Arita, Asao, Tanaka, Higuchi, Kuroda, Kaneko, Munakata, Endo, Fujita, Sugamura: "Cloning of a novel signal-transducing adaptor molecule containing an SH3 domain and ITAM." in: Biochemical and biophysical research communications, Vol. 225, Issue 3, pp. 1035-9, 1996
ImagesProduct Details anti-STAM Antibody Target Details STAM Application Details Handling References for anti-STAM antibody (ABIN350877) back to top