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anti-Human Villin 1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Villin 1 Antibody (supplied by: Log in to see )

Antigen

Villin 1 (VIL1) Antibodies
show synonyms for this antigen

D2S1471
VIL
Vil
vil1
MGC64339
VIL1
vil1l
zgc:55779
villin 1
villin 1 S homeolog
VIL1
Vil1
vil1.S
vil1

Epitope

AA 180-207, N-Term
Alternatives

Reactivity

Human, Mouse (Murine)
Alternatives

Host

Rabbit
Alternatives

Clonality

Application

Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Alternatives

Options

Supplier

Supplier Product No.

1 reference available

Available images

Western Blotting (WB) image for anti-Villin 1 (VIL1) (AA 180-207), (N-Term) antibody (ABIN390742)

Immunohistochemistry (IHC) image for anti-Villin 1 (VIL1) (AA 180-207), (N-Term) antibody (ABIN390742)

Flow Cytometry (FACS) image for anti-Villin 1 (VIL1) (AA 180-207), (N-Term) antibody (ABIN390742)

Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Villin 1 (VIL1) (AA 180-207), (N-Term) antibody (ABIN390742)

Catalog No. ABIN390742

$ 385.00
Plus shipping costs $45.00

Quantity

Price

80 μL

$147.40

400 μL

$385.00

Product detail page

Relevance Score	ABIN	Application	Conjugate	Host	Isotype	Epitope	Supplier	Clonality	References
16.614061	ABIN4365288	IHC IHC (p)		Rabbit	IgG		Log in to see	Polyclonal	1
16.614061	ABIN4365289	IHC IHC (p) WB		Rabbit	IgG		Log in to see	Polyclonal	1
14.533073	ABIN453459	EIA FACS IHC (p) WB		Rabbit		N-Term	Log in to see	Polyclonal	1
13	ABIN3042343	IHC (p) WB		Rabbit	IgG	AA 770-799, C-Term	Log in to see	Polyclonal	0
12.114061	ABIN4365299	CyTOF ELISA FACS ICC IF IHC IHC (p) WB		Mouse	IgG1		Log in to see	3E5G11	0
10	ABIN5590731	IF IHC (p) WB		Rabbit	IgG		Log in to see	Polyclonal	0
9.114061	ABIN442795	ICC IF IHC (p) IHC WB		Rabbit	IgG	N-Term	Log in to see	Polyclonal	0
7	ABIN5590732	IF IHC (p)		Rabbit	IgG		Log in to see	Polyclonal	0
6.114062	ABIN4251471	ELISA IHC IHC (p) WB		Mouse			Log in to see	5E3B2	0
4	ABIN400765	IHC (p)		Mouse	IgG1		Log in to see	3F392	0
4	ABIN5590729	IHC (p)		Mouse	IgG1		Log in to see	3F392	0
4	ABIN955515	IHC (p)		Mouse	IgG1		Log in to see	CWWB1	0
1	ABIN5954394	ELISA IHC (p) WB		Mouse	IgG2b		Log in to see	5E3B2	0
1	ABIN5954393	ELISA FACS ICC IHC (p) WB		Mouse	IgG1		Log in to see	3E5G11	0
-10.419012	ABIN4964644	FACS ICC IF IHC (p) WB		Mouse	IgG1 kappa		Log in to see	VIL1-1325	0
-10.419012	ABIN4964641	FACS ICC IF IHC (p) WB		Mouse	IgG1 kappa		Log in to see	VIL1-1314	0
-10.419012	ABIN4964648	CyTOF FACS ICC IF IHC (p) WB		Mouse	IgG1 kappa		Log in to see	VIL1-1325	0
-10.419012	ABIN4964666	CyTOF FACS ICC IF IHC (p) WB		Mouse	IgG1 kappa		Log in to see	VIL1-1314	0
-10.419012	ABIN5947920	FACS ICC IF IHC IHC (p)	Janelia Fluor® 549	Mouse	IgG1 kappa	AA 179-311	Log in to see	VIL1-1314	0
-10.419012	ABIN5947921	FACS ICC IF IHC IHC (p)	Janelia Fluor® 646	Mouse	IgG1 kappa	AA 179-311	Log in to see	VIL1-1314	0

More product categories related to Villin 1 Antibodies

Top referenced anti-Villin 1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Human Polyclonal VIL1 Primary Antibody for IHC, IHC (p) - ABIN4365288 (1 Publications): Kiflemariam, Andersson, Asplund, Pontén, Sjöblom: Scalable in situ hybridization on tissue arrays for validation of novel cancer and tissue-specific biomarkers. in PLoS ONE 2012 (PubMed)
Human Polyclonal VIL1 Primary Antibody for IHC, IHC (p) - ABIN4365289 (1 Publications): Andersson, Konrad, Ashok, Pontén, Hober, Asplund: Antibodies biotinylated using a synthetic Z-domain from protein A provide stringent in situ protein detection. in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2013 (PubMed)

Similar anti-Villin 1 Antibodies

Application / Reactivity	Human	Mouse (Murine)	Rat (Rattus)	Cow (Bovine)	Dog (Canine)	Guinea Pig	Horse (Equine)	Rabbit	Zebrafish (Danio rerio)
Immunofluorescence (IF)	40 antibody	1 antibody	1 antibody
Western Blotting (WB)	82 antibody	11 antibody	7 antibody	1 antibody	1 antibody	1 antibody	1 antibody	1 antibody	1 antibody
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))	49 antibody	4 antibody	2 antibody
Immunohistochemistry (IHC)	31 antibody	4 antibody	3 antibody
Flow Cytometry (FACS)	47 antibody	3 antibody
Immunocytochemistry (ICC)	38 antibody	1 antibody	1 antibody
Enzyme Immunoassay (EIA)	1 antibody	1 antibody
Immunoprecipitation (IP)	4 antibody
ELISA	25 antibody	3 antibody	3 antibody
Immunochromatography (IC)	1 antibody
Cytometry by Time of Flight (CyTOF)	3 antibody
Immunofluorescence (fixed cells) (IF/ICC)	3 antibody

General
Antigen	Villin 1 (VIL1) Antibodies
Epitope	AA 180-207, N-Term Alternatives
	(9), (6), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity	Human, Mouse (Murine) Alternatives
	(57), (9), (6), (1), (1), (1), (1), (1), (1)
Host	Rabbit Alternatives
	(33), (24)
Clonality (Clone)	Polyclonal ( )
Application	Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB) Alternatives (48), (23), (22), (9), (8), (5), (4), (4), (3), (1), (1)
	1 reference available
Supplier	Log in to see
Product details Target Details Application Details Handling References Images
Specificity	This Villin-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186~216 amino acids from the N-terminal region of human VILLIN1.
Predicted Reactivity	Cow (Bovine),Pig (Porcine)
Purification	This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen	This Villin-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-207 AA from the N-terminal region of human Villin-1.
Clone	RB17824
Isotype	Ig Fraction

Target Details Product details Application Details Handling References Images back to top
Antigen
Alternative Name	Villin-1 (VIL1 Antibody Abstract)
Background	Villin-1 is a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Synonyms: Villin-1, VIL, VIL1
Molecular Weight	92695 DA
Gene ID	7429
UniProt	P09327
Pathways	EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
Application Details Product details Target Details Handling References Images back to top
Application Notes	WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions	For Research Use only
Handling Product details Target Details Application Details References Images back to top
Format	Liquid
Buffer	PBS with 0.09 % (W/V) sodium azide
Preservative	Sodium azide
Precaution of Use	This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage	4 °C/-20 °C
Storage Comment	Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date	6 months
References Product details Target Details Application Details Handling Images back to top
Background publications	Yamamichi, Inada, Furukawa, Sakurai, Tando, Ishizaka, Haraguchi, Mizutani, Fujishiro, Shimomura, Oka, Ichinose, Tsutsumi, Omata, Iba: "Cdx2 and the Brm-type SWI/SNF complex cooperatively regulate villin expression in gastrointestinal cells." in: Experimental cell research, Vol. 315, Issue 10, pp. 1779-89, 2009
Images Product details Target Details Application Details Handling References back to top
Supplier Images	Western blot analysis of Villin-1 Antibody (N-term) (ABIN390742) in mouse kidney tiss... Formalin-fixed and paraffin-embedded human colon carcinoma reacted with Villin-1 Anti... Flow cytometric analysis of WiDr cells using Villin-1 Antibody (N-term)(bottom histog... Overlay histogram showing Hela cells stained with ABIN2841007(green line). The cells ... ABIN2841007 staining Villin-1 in human kidney tissue sections by Immunohistochemistry... All lanes : Anti-Villin-1 Antibody (N-term) at 1:1000 dilution Lane 1: Caco2 whole ce... All lanes : Anti-Villin-1 Antibody (N-term) at 1:2000 dilution Lane 1: HT-29 whole ce... All lanes : Anti-Villin-1 Antibody (N-term) at 1:8000 dilution Lane 1: HT-29 whole ce... All lanes : Anti-Villin-1 Antibody (N-term) at 1:2000 dilution Lane 1: HepG2 whole ce... Images for product: anti-Villin 1 (VIL1) (AA 180-207), (N-Term) antibody Western blot analysis of Villin-1 Antibody (N-term) (ABIN390742) in mouse kidney tissue lysates (35 µg/lane). VIL1 (arrow) was detected using the purified polyclonal antibody. Formalin-fixed and paraffin-embedded human colon carcinoma reacted with Villin-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Flow cytometric analysis of WiDr cells using Villin-1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Overlay histogram showing Hela cells stained with ABIN2841007(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (ABIN2841007, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Dilution: 1:25 ABIN2841007 staining Villin-1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Dilution: 1:25 All lanes : Anti-Villin-1 Antibody (N-term) at 1:1000 dilution Lane 1: Caco2 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Dilution: 1:1000 All lanes : Anti-Villin-1 Antibody (N-term) at 1:2000 dilution Lane 1: HT-29 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: COLO 205 whole cell lysate Lane 4: Mouse colon lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Dilution: 1:2000 All lanes : Anti-Villin-1 Antibody (N-term) at 1:8000 dilution Lane 1: HT-29 whole cell lysate Lane 2: SW480 whole cell lysate Lane 3: Mouse colon lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Dilution: 1:8000 All lanes : Anti-Villin-1 Antibody (N-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: Mouse kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Dilution: 1:2000