anti-Mouse (Murine) Villin 1 antibody for Enzyme Immunoassay

Recommended Villin 1 Antibody (supplied by: Log in to see )

Antigen
Villin 1 (VIL1) Antibodies
  • D2S1471
  • VIL
  • Vil
  • vil1
  • MGC64339
  • VIL1
  • vil1l
  • zgc:55779
  • villin 1
  • villin 1 S homeolog
  • VIL1
  • Vil1
  • vil1.S
  • vil1
Epitope
N-Term
9
6
6
5
3
3
2
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
102
10
7
1
1
1
1
1
1
Host
Rabbit
67
35
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
80
48
46
40
38
31
25
4
3
3
1
Supplier
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Catalog No. ABIN453459
$ 390.50
Plus shipping costs $45.00

General

Antigen Villin 1 (VIL1) Antibodies
Epitope N-Term
(9), (6), (6), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(102), (10), (7), (1), (1), (1), (1), (1), (1)
Host Rabbit
(67), (35)
Clonality
Polyclonal
Conjugate Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(80), (48), (46), (40), (38), (31), (25), (4), (3), (3), (1)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Specificity This antibody recognizes Villin-1.
Cross-Reactivity (Details) Species reactivity (expected):Bovine, Pig.
Species reactivity (tested):Human, Mouse.
Purification Saturated Ammonium Sulfate (SAS) precipitation
Immunogen KLH conjugated synthetic peptide selected from the N-terminal region of human VILLIN1

Target Details

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Antigen
Alternative Name Villin-1 (VIL1 Antibody Abstract)
Background Villin-1 is a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments.Synonyms: VIL1
Gene ID 7429
NCBI Accession NP_009058
UniProt P09327
Pathways EGFR Signaling Pathway, Regulation of Actin Filament Polymerization

Application Details

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Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry on paraffin sections: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % (W/V) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References

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Background publications

Yamamichi, Inada, Furukawa, Sakurai, Tando, Ishizaka, Haraguchi, Mizutani, Fujishiro, Shimomura, Oka, Ichinose, Tsutsumi, Omata, Iba: "Cdx2 and the Brm-type SWI/SNF complex cooperatively regulate villin expression in gastrointestinal cells." in: Experimental cell research, Vol. 315, Issue 10, pp. 1779-89, 2009

Images

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Supplier Images
Flow Cytometry (FACS) image for anti-Villin 1 (VIL1) (N-Term) antibody (ABIN453459) Flow Cytometric analysis of WiDr cells using Villin-1 Antibody (N-term)  (bottom hist...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Villin 1 (VIL1) (N-Term) antibody (ABIN453459) Formalin-fixed and paraffin-embedded human colon carcinoma reacted with Villin-1 Anti...
Western Blotting (WB) image for anti-Villin 1 (VIL1) (N-Term) antibody (ABIN453459) Western blot analysis of Villin-1 Antibody (N-term) in mouse kidney tissue lysates (3...