anti-Rat (Rattus) Villin 1 antibody for Immunohistochemistry

Recommended Villin 1 Antibody (supplied by: Log in to see )

Antigen
Villin 1 (VIL1) Antibodies
  • D2S1471
  • VIL
  • Vil
  • vil1
  • MGC64339
  • VIL1
  • vil1l
  • zgc:55779
  • villin 1
  • villin 1 S homeolog
  • VIL1
  • Vil1
  • vil1.S
  • vil1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
57
9
5
1
1
1
1
1
1
Host
Rabbit
33
24
Clonality
Polyclonal
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
48
22
21
10
9
5
4
4
3
1
1
Options
Supplier
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Supplier Product No.
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Available images

Western Blotting (WB) image for anti-Villin 1 (VIL1) antibody (ABIN6265977)
Immunohistochemistry (IHC) image for anti-Villin 1 (VIL1) antibody (ABIN6265977)
Catalog No. ABIN6265977
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
4 ABIN1876682 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.8744183 ABIN442795 ICC IF IHC (p) IHC WB Rabbit IgG N-Term Log in to see Polyclonal 0

General

Antigen Villin 1 (VIL1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(57), (9), (5), (1), (1), (1), (1), (1), (1)
Host Rabbit
(33), (24)
Clonality
Polyclonal
Application ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(48), (22), (21), (10), (9), (5), (4), (4), (3), (1), (1)
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Product details

Target Details Application Details Handling Images
Specificity VIL1 Antibody detects endogenous levels of total VIL1
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthesized peptide derived from human VIL1
Isotype IgG

Target Details

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Antigen
Alternative Name VIL1 (VIL1 Antibody Abstract)
Background

Description: Epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA), binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.

Gene: VIL1
Molecular Weight 93kDa
Gene ID 7429
UniProt P09327
Pathways EGFR Signaling Pathway, Regulation of Actin Filament Polymerization

Application Details

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Application Notes WB 1:500-1:2000 IHC 1:50-1:200
Restrictions For Research Use only

Handling

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Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

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Supplier Images
Western Blotting (WB) image for anti-Villin 1 (VIL1) antibody (ABIN6265977) Western blot analysis of Mouse liver tissue lysates, using VIL1 Antibody. The lane on...
Immunohistochemistry (IHC) image for anti-Villin 1 (VIL1) antibody (ABIN6265977) ABIN6277617 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehy...