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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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4 | ABIN1876682 | IHC WB | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
2.060627 | ABIN442795 | ICC IF IHC (p) IHC WB | Rabbit | IgG | N-Term | Log in to see | Polyclonal | 0 |
General |
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Antigen | Villin 1 (VIL1) Antibodies |
Reactivity | Human, Mouse (Murine), Rat (Rattus) Alternatives |
Host | Rabbit Alternatives |
Clonality | |
Conjugate | Un-conjugated Alternatives |
Application |
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Alternatives
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Supplier | Log in to see |
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Specificity | VIL1 Antibody detects endogenous levels of total VIL1 |
Cross-Reactivity | Human, Mouse (Murine), Rat (Rattus) |
Purification | The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific). |
Immunogen | A synthesized peptide derived from human VIL1 |
Isotype | IgG |
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Antigen | |
Alternative Name | VIL1 (VIL1 Antibody Abstract) |
Background |
Description: Epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA), binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination. Gene: VIL1 |
Molecular Weight | 93kDa |
Gene ID | 7429 |
UniProt | P09327 |
Pathways | EGFR Signaling Pathway, Regulation of Actin Filament Polymerization |
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Application Notes | WB 1:500-1:2000 IHC 1:50-1:200 |
Restrictions | For Research Use only |
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Format | Liquid |
Concentration | 1 mg/mL |
Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol. |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Storage | -20 °C |
Storage Comment | Store at -20 °C.Stable for 12 months from date of receipt |
Expiry Date | 12 months |
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