anti-Dog (Canine) EDEM1 antibody for Western Blotting

Recommended EDEM1 Antibody (supplied by: Log in to see )

Antigen
ER Degradation Enhancer, Mannosidase alpha-Like 1 (EDEM1) Antibodies
  • zgc:55816
  • edem
  • EDEM1
  • EDEM
  • A130059K23Rik
  • mKIAA0212
  • RGD1563633
  • ER degradation enhancer, mannosidase alpha-like 1
  • ER degradation enhancing alpha-mannosidase like protein 1
  • ER degradation-enhancing alpha-mannosidase-like protein 1
  • ER degradation enhancing alpha-mannosidase like protein 1 L homeolog
  • edem1
  • EDEM1
  • LOC100637820
  • edem1.L
  • Edem1
Epitope
N-Term
4
3
2
2
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Human, Mouse (Murine), Rabbit, Rat (Rattus), Yeast (Saccharomyces cerevisiae)
18
11
6
3
3
3
3
Host
Rabbit
14
4
Clonality
Polyclonal
Application
Western Blotting (WB)
16
8
2
1
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Available images

Catalog No. ABIN2782826
$ 319.00
Plus shipping costs $45.00

General

Antigen ER Degradation Enhancer, Mannosidase alpha-Like 1 (EDEM1) Antibodies
Epitope N-Term
(4), (3), (2), (2), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Human, Mouse (Murine), Rabbit, Rat (Rattus), Yeast (Saccharomyces cerevisiae)
(18), (11), (6), (3), (3), (3), (3)
Host Rabbit
(14), (4)
Clonality
Polyclonal
Application Western Blotting (WB)
(16), (8), (2), (1)
Pubmed 1 reference available
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Product Details anti-EDEM1 Antibody

Target Details EDEM1 Application Details Handling References for anti-EDEM1 antibody (ABIN2782826) Images
Blocking Peptide Blocking peptide for this product available: ABIN976126
Sequence MAHAFPQDEL NPIHCRGRGP DRGDPSNLNI NDVLGNYSLT LVDALDTLAI
Predicted Reactivity Cow: 100%, Dog: 93%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 82%
Characteristics This is a rabbit polyclonal antibody against EDEM1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human EDEM1
Plasmids, Primers & others

Target Details EDEM1

Product Details anti-EDEM1 Antibody Application Details Handling References for anti-EDEM1 antibody (ABIN2782826) Images back to top
Antigen
Alternative Name EDEM1 (EDEM1 Antibody Abstract)
Background EDEM1 belongs to the glycosyl hydrolase 47 family. It extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-dependent manner.
Alias Symbols: EDEM, KIAA0212
Protein Interaction Partner: HLA-B, SQSTM1, UBC, MMS19, SSR1, ASGR1, DERL1, DNAJC10, DERL2, SEC61B, SEL1L, RHO, CANX, RCOM_2159910, ASGR2, ELAVL1, BZW1,
Protein Size: 657
Molecular Weight 74 kDa
Gene ID 9695
NCBI Accession NM_014674, NP_055489
UniProt Q92611
Pathways ER-Nucleus Signaling

Application Details

Product Details anti-EDEM1 Antibody Target Details EDEM1 Handling References for anti-EDEM1 antibody (ABIN2782826) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 657 AA

Restrictions For Research Use only

Handling

Product Details anti-EDEM1 Antibody Target Details EDEM1 Application Details References for anti-EDEM1 antibody (ABIN2782826) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EDEM1 antibody (ABIN2782826)

Product Details anti-EDEM1 Antibody Target Details EDEM1 Application Details Handling Images back to top
Background publications

Zuber, Cormier, Guhl, Santimaria, Hebert, Roth: "EDEM1 reveals a quality control vesicular transport pathway out of the endoplasmic reticulum not involving the COPII exit sites." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 11, pp. 4407-12, 2007

Images

Product Details anti-EDEM1 Antibody Target Details EDEM1 Application Details Handling References for anti-EDEM1 antibody (ABIN2782826) back to top
Supplier Images
Western Blotting (WB) image for anti-ER Degradation Enhancer, Mannosidase alpha-Like 1 (EDEM1) (N-Term) antibody (ABIN2782826) WB Suggested Anti-EDEM1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Pos...