anti-Cow (Bovine) LPAR3 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended LPAR3 Antibody

Antigen
Lysophosphatidic Acid Receptor 3 (LPAR3) Antibodies
  • EDG7
  • Edg-7
  • GPCR
  • HOFNH30
  • LP-A3
  • LPA3
  • RP4-678I3
  • Edg7
  • lpA3
  • lysophosphatidic acid receptor 3
  • LPAR3
  • LOC100038016
  • Lpar3
Epitope
Cytoplasmic Domain
15
9
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Bat, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Rat (Rattus)
94
49
40
2
1
1
1
1
1
1
1
Host
Rabbit
98
1
Clonality
Polyclonal
Conjugate
This LPAR3 antibody is un-conjugated
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
59
35
21
21
20
13
7
3
3
2
2
Options

Available images

Catalog No. ABIN1048536
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
7 ABIN1048537 IHC IHC (p) Rabbit Cytoplasmic Domain Polyclonal 0

General

Antigen Lysophosphatidic Acid Receptor 3 (LPAR3) Antibodies
Epitope Cytoplasmic Domain
(15), (9), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bat, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Rat (Rattus)
(94), (49), (40), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(98), (1)
Clonality
Polyclonal
Conjugate This LPAR3 antibody is un-conjugated
(2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(59), (35), (21), (21), (20), (13), (7), (3), (3), (2), (2)
Supplier

Product Details anti-LPAR3 Antibody

Target Details LPAR3 Application Details Handling Images
Brand IHC-plus™
Specificity Human EDG7. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Horse, Opossum, Platypus (100%) Panda (94%) Pig, Turkey, Chicken (88%) Xenopus (81%).
Purification Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human EDG7. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Horse, Opossum, Platypus (100%), Panda (94%), Pig, Turkey, Chicken (88%), Xenopus (81%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details LPAR3

Product Details anti-LPAR3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name LPAR3 / LPA3 / EDG7 (LPAR3 Antibody Abstract)
Background Name/Gene ID: LPAR3
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR

Synonyms: LPAR3, EDG7, Edg-7, LPA receptor 3, HOFNH30, LPA3, RP4-678I3, LP-A3, LPA receptor EDG7, LPA-3
Gene ID 23566
Pathways Regulation of Cell Size

Application Details

Product Details anti-LPAR3 Antibody Target Details LPAR3 Handling Images back to top
Application Notes Approved: IHC, IHC-P (10 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-LPAR3 Antibody Target Details LPAR3 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-LPAR3 Antibody Target Details LPAR3 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Lysophosphatidic Acid Receptor 3 (LPAR3) (Cytoplasmic Domain) antibody (ABIN1048536) Anti-EDG7 antibody ABIN1048536 IHC staining of human brain, amygdala. Immunohistoche...
Immunohistochemistry (IHC) image for anti-Lysophosphatidic Acid Receptor 3 (LPAR3) (Cytoplasmic Domain) antibody (ABIN1048536) Anti-LPAR3 / LPA3 / EDG7 antibody IHC of human Breast, Carcinoma. Immunohistochemistr...