anti-Dog (Canine) Mesoderm Posterior 1 Homolog (Mouse) antibody for Western Blotting

Recommended Mesoderm Posterior 1 Homolog (Mouse) Antibody (supplied by: Log in to see )

Antigen
Mesoderm Posterior 1 Homolog (Mouse) (MESP1) Antibodies
  • bHLHc5
  • mesp
  • mesp1
  • mespa
  • CMESO-1
  • CMESO2
  • MESP1
  • mesoderm posterior 1
  • mesoderm posterior bHLH transcription factor 1
  • mesoderm posterior 1 homolog (mouse)
  • mesoderm posterior homolog B
  • mesoderm posterior homolog B L homeolog
  • Mesp1
  • MESP1
  • mesp1
  • mespb
  • mespb.L
Epitope
N-Term
18
12
10
8
7
7
6
2
2
2
1
1
1
1
Reactivity
Dog (Canine), Human
72
43
2
1
1
Host
Rabbit
74
17
Clonality
Polyclonal
Conjugate
Un-conjugated
5
5
5
5
5
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
87
46
21
18
4
4
3
2
2
1
Options
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Catalog No. ABIN2781073
$ 319.00
Plus shipping costs $45.00

General

Antigen Mesoderm Posterior 1 Homolog (Mouse) (MESP1) Antibodies
Epitope N-Term
(18), (12), (10), (8), (7), (7), (6), (2), (2), (2), (1), (1), (1), (1)
Reactivity Dog (Canine), Human
(72), (43), (2), (1), (1)
Host Rabbit
(74), (17)
Clonality
Polyclonal
Conjugate Un-conjugated
(5), (5), (5), (5), (5), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(87), (46), (21), (18), (4), (4), (3), (2), (2), (1)
Pubmed 5 references available
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Product details

Target Details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN979994
Sequence SLVSSPDSWG STPADSPVAS PARPGTLRDP RAPSVGRRGA RSSRLGSGQR
Predicted Reactivity Dog: 85%, Human: 100%
Characteristics This is a rabbit polyclonal antibody against MESP1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human MESP1

Target Details

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Antigen
Alternative Name MESP1 (MESP1 Antibody Abstract)
Background MESP1 is a transcription factor. MESP1 plays a role in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. MESP1 defines the rostrocaudal patterning of the somites by participating in distinct Notch pathways.
Alias Symbols: MGC10676, bHLHc5
Protein Size: 268
Molecular Weight 28 kDa
Gene ID 55897
NCBI Accession NM_018670, NP_061140
UniProt Q9BRJ9
Pathways Regulation of Muscle Cell Differentiation

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 268 AA

Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Product cited in:

Tan, Han, Gu, Chen, Wang, Ma, Wu, Feng, Zhang, Wang, Hu, Li, Hao, Zhou: "Generation of clinical-grade functional cardiomyocytes from human embryonic stem cells in chemically defined conditions." in: Journal of tissue engineering and regenerative medicine, 2016

Hu, Wang, Jiao, Liu, Zhao, Zhou, Zhang, Forde, Wang, Wang, Baylink, Zhang, Gao, Yang, Chen, Ma: "Patient-specific cardiovascular progenitor cells derived from integration-free induced pluripotent stem cells for vascular tissue regeneration." in: Biomaterials, Vol. 73, pp. 51-9, 2015

Cao, Liang, Huang, Wang, Chen, Chen, Yang: "Highly efficient induction and long-term maintenance of multipotent cardiovascular progenitors from human pluripotent stem cells under defined conditions." in: Cell research, Vol. 23, Issue 9, pp. 1119-32, 2013 (1:100).

Blin, Nury, Stefanovic, Neri, Guillevic, Brinon, Bellamy, Rücker-Martin, Barbry, Bel, Bruneval, Cowan, Pouly, Mitalipov, Gouadon, Binder, Hagège, Desnos, Renaud, Menasché, Pucéat: "A purified population of multipotent cardiovascular progenitors derived from primate pluripotent stem cells engrafts in postmyocardial infarcted nonhuman primates." in: The Journal of clinical investigation, Vol. 120, Issue 4, pp. 1125-39, 2010

Background publications

David, Brenner, Stieber, Schwarz, Brunner, Vollmer, Mentele, Müller-Höcker, Kitajima, Lickert, Rupp, Franz: "MesP1 drives vertebrate cardiovascular differentiation through Dkk-1-mediated blockade of Wnt-signalling." in: Nature cell biology, Vol. 10, Issue 3, pp. 338-45, 2008

Images

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Supplier Images
Western Blotting (WB) image for anti-Mesoderm Posterior 1 Homolog (Mouse) (MESP1) (N-Term) antibody (ABIN2781073) WB Suggested Anti-MESP1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Po...